[en] Traditional Malagasy farmers have developed a range of biological methods to restrict plant diseases without reliance on external or synthetic inputs. Five common Malagasy traditional practices demonstrated to be efficient against potato crop bacterial disease in experimental fields have been investigated for their antibacterial (i.e. bacteriostatic and bactericidal effects) and anti-virulence (i.e. antiquorum sensing and anti-biofilm) activities against two phytopathogens, Pseudomonas aeruginosa and Ralstonia solanacearum. Results show that polar (methanolic) extracts of recipes exert anti-virulence activities rather than bacteriostatic and/or bactericidal activities. Indeed, three recipes (R5, R7 and R9) reduce the expression of QS-dependent virulence factors whereas only recipe (R5) exhibit antibiofilm activities without affecting bacterial growth. R4 and R6 were not active, suggesting other bacterial targets and/or other bioactivity properties. Innovative approaches, inspired from ancestral practices, should be considered in the struggle against infectious diseases to limit the overuse of antibiotics for controlling infectious plant diseases and to reduce the overspread of multidrug resistant bacteria.
Disciplines :
Biotechnology
Author, co-author :
Rasamiravaka, Tsiry; Laboratory of Biotechnology and Microbiology, Department of Applied and Fundamental Biochemistry, Faculty of Science, University of Antananarivo (UA), Antananarivo, Madagascar
Rajaonarivelo Philémon Jacob; Laboratory of Biotechnology and Microbiology, Department of Applied and Fundamental Biochemistry, Faculty of Science, University of Antananarivo (UA), Antananarivo, Madagascar ; Laboratoire de Microbiologie, Département de Biodiversité, Institut Malgache de Recherches Appliquées (IMRA), Avarabohitra, Antananarivo, Madagascar
Rabemanantsoa, Christian; Laboratoire de Microbiologie, Département de Biodiversité, Institut Malgache de Recherches Appliquées (IMRA), Avarabohitra, Antananarivo, Madagascar
Andrianarisoa, Blandinef; Laboratory of Biotechnology and Microbiology, Department of Applied and Fundamental Biochemistry, Faculty of Science, University of Antananarivo (UA), Antananarivo, Madagascar
Duez, Pierre; Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), Mons, Belgium
El Jaziri, Mondher; Laboratory of Plant Biotechnology (LBV), Free University of Brussels (ULB), Gosselies, Belgium
Language :
English
Title :
Malagasy traditional treatments of infectious plant diseases exert anti-virulence activities against pseudomonas aeruginosa and Ralstonia solanacearum
Publication date :
February 2018
Journal title :
Journal of Microbiology, Biotechnology and Food Sciences
Acknowledgments: This research was supported by the project PIC-Madagascar 2009 of the ARES-CCD (Académie de Recherche et d’Enseignement Supérieur-Commission Coopération au Développement, Belgium) through the postdoctoral fellowship “ELAN 2015-2016” and by David and Alice Van Buuren foundation, Université Libre de Bruxelles. We would like to thank Professor Junichi Kato, Hiroshima University (Department of Molecular Biotechnology), Japan, for kindly providing plasmids pβ01, pβ02; Professor Helmut Görisch, Technische Universität Berlin (Fachgebiet Technische Biochemie, Institut für Biotechnologie), Germany, for providing plasmid pTB4124; Mrs L. M. Razanakoto and Prof. H. Jijakli (Gembloux Agro-Bio Tech, University of Liège, Belgium) for providing the R. solanacearum strain.
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