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AgNOR proteins from morphologically intact isolated nucleoli.
Vandelaer, M.; Thiry, Marc; Goessens, Guy
1999In Life Sciences, 64 (22), p. 2039-47
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Keywords :
Animals; Blotting, Western; Carcinoma, Ehrlich Tumor/metabolism/ultrastructure; Cell Nucleolus/metabolism/ultrastructure; Mice; Microscopy, Immunoelectron; Molecular Weight; Neoplasm Proteins/chemistry/isolation & purification/metabolism; Nuclear Proteins/chemistry/isolation & purification/metabolism; Silver Staining; Tumor Cells, Cultured
Abstract :
[en] AgNOR staining has been proposed as a useful tool for the diagnosis and prognosis of cancer. The AgNOR proteins, however, have not yet been clearly identified and characterized, possibly due to the partial character of the results obtained when studying the proteins extracted from altered nucleoli isolated by "standard" methods. In the present study, we analysed, on western blots, the AgNOR staining profiles obtained with protein extracts from Ehrlich tumor cell nucleoli isolated by a recent procedure that preserves the nucleolar ultrastructure. In addition to the well-known C23 and B23 protein bands, we readily detected an extra band at approximately 125 Kda. By immunoblotting, we showed that this polypeptide may be related to the nucleolar phosphoprotein pp135 evidenced in rat-cell nucleoli. By immunoelectron microscopy, we detected this protein in the dense fibrillar component and fibrillar center of the nucleoli as well as the coiled bodies. The distribution coincides with the cytochemical AgNOR staining pattern obtained at the ultrastructural level.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Vandelaer, M.
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Goessens, Guy ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
AgNOR proteins from morphologically intact isolated nucleoli.
Publication date :
1999
Journal title :
Life Sciences
ISSN :
0024-3205
Publisher :
Pergamon Press, Oxford, United Kingdom
Volume :
64
Issue :
22
Pages :
2039-47
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

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