Nucleic acid compartmentalization within the cell nucleus by in situ transferase-immunogold techniques.

Thiry, Marc

doi:10.1002/jemt.1070310103

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Nucleic acid compartmentalization within the cell nucleus by in situ transferase-immunogold techniques.

Thiry, Marc

1995 • In Microscopy Research and Technique, 31 (1), p. 4-21

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https://hdl.handle.net/2268/29312

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10.1002/jemt.1070310103

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7542939

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Keywords :

Cell Compartmentation; Cell Line; Cell Nucleus/chemistry/ultrastructure; DNA/analysis/ultrastructure; DNA Nucleotidylexotransferase/analysis/ultrastructure; Humans; Immunohistochemistry/methods; In Situ Hybridization; Microscopy, Immunoelectron/methods; RNA/analysis/ultrastructure; RNA Nucleotidyltransferases/analysis/ultrastructure; Tumor Cells, Cultured

Abstract :

[en] In the present review, we report on recent results obtained by in situ transferase-immunogold techniques as to the ultrastructural distribution of DNA and RNA within the cell nucleus. Special emphasis is placed on the various nucleolar components and the various enigmatic structures of the extranucleolar region: interchromatin granules, coiled bodies, and simple nuclear bodies. These data are discussed in the light of our current understanding of the functional organization of the cell nucleus.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Language :

English

Title :

Nucleic acid compartmentalization within the cell nucleus by in situ transferase-immunogold techniques.

Publication date :

1995

Journal title :

Microscopy Research and Technique

ISSN :

1059-910X

eISSN :

1097-0029

Publisher :

Wiley Liss, Inc., Hoboken, United States - New Jersey

Volume :

Issue :

Pages :

4-21

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Peer Reviewed verified by ORBi

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since 25 November 2009

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Bibliography

Andrade L., Chan E.K., Raska I., Peebles C., Roos G., Tan E.M. (1991) Human autoantibody to a novel protein of the nuclear coiled body: Immunological characterization and cDNA cloning of p80‐coilin. J. Exp. Med. 173:1407-1419.
Andrade L., Tan E.M., Chan E.K.L. (1993) Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation. Proc. Natl. Acad. Sci. U.S.A. 90:1947-1951.
Bachellerie J.‐P., Nicoloso M., Zalta J.‐P. (1977) Nucleolar chromatin in chinese hamster ovary cells. Topographical distribution of ribosomal DNA sequences and isolation of ribosomal transcription complexes. Eur. J. Biochem. 79:23-32.
Baserga S., Yang X., Steitz J.A. (1991) An intact Box C sequence in the U3 SnRNA is requiED for binding of fibrillarin, the protein common to the major family of nucleolar SnRNPs. EMBO J. 10:2645-2651.
Bendayan M. (1981) Electron microscopical localization of nucleic acids by means of nuclease‐gold complexes. Histochem. J. 13:699-710.
Bendayan M. (1981) Ultrastructural localization of nucleic acids by the use of enzyme‐gold complexes. J. Histochem. Cytochem. 29:531-541.
Bendayan M. (1984) Enzyme‐gold electron microscopic cytochemistry: A new affinity approach for the ultrastructural localization of macromolecules. J. Electron Microsc. Tech. 1:349-372.
Bendayan M., Puvion E. (1983) Ultrastructural detection of RNA: Complementarity of high‐resolution autoradiography and of RNase‐gold method. J. Ultrastruct. Res. 83:274-283.
Bendayan M., Stephens H. (1984) Double labelling cytochemistry applying the protein A–gold technique. Immunolabelling of Electron Microscopy , J. Polak, I. Varndell, Elsevier, Amsterdam, New York; 143-154.
Bendayan M., Nanci A., Kan F. (1987) Effect of tissue processing on colloidal gold cytochemistry. J. Histochem. Cytochem. 35:983-996.
Bernhard W. (1969) A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res. 27:250-265.
Bollum F.J. (1963) Intermediate states in enzymatic DNA synthesis. Journal of Cellular and Comparative Physiology 62:61-71.
Bollum F.J. (1974) Terminal deoxynucleotidyl transferase. The Enzymes , P. D. Boyer, Academic Press, New York; 10:145-171.
Bouteille M., Laval M., Dupuy‐Coin A.M. (1974) Localization of nuclear functions as revealed by ultrastructural autoradiography and cytochemistry. The Cell Nucleus , H. Busch, Academic Press, New York; 1:5-64.
Bouteille M., Dupuy‐Coin A.M., Moyne G. (1975) Methods for localization of proteins and nucleoproteins in the cell nucleus by high resolution autoradiography and cytochemistry. Methods in Enzymology , J. G. Hardmann, B. W. O'Malley, Academic Press, New York; 40:3-41.
Bouteille M., Hernandez‐Verdun D., Dupuy‐Coin A.M., Bourgeois C.A. (1982) Nucleoli and nucleolar‐related structures in normal, infected and drug‐treated cells. The nucleolus , E. Jordan, C. Cullis, Cambridge University Press, London and New York; 15:179-211.
Brasch K., Ochs R. (1992) Nuclear bodies: A newly EDiscoveED organelle. Exp. Cell Res. 202:211-223.
Carmo‐Fonseca M., Pfiefer K., Schröder M.C., Fatima Vaz M., Fonseca J.E., Muller W.E.G., Bachmann M. (1989) Identification of La ribonucleoproteins as a component of interchromatin granules. Exp. Cell Res. 185:73-85.
Carmo‐Fonseca M., Pepperkok R., Carvalmo M.T., Lamond A.I. (1992) Transcription‐dependent colocalization of the U1, U2, U4/U6, and U5 SnRNPS in coiled bodies. J. Cell Biol. 117:1-14.
Carmo‐Fonseca M., Ferreira J., Lamond A.I. (1993) Assembly of SnRNP‐containing coiled bodies is regulated in interphase and mitosis—evidence that the coiled body is a kinetic nuclear structure. J. Cell Biol. 120:841-852.
Carter K., Bowman D., Carrington W., Fogarty K., McNeil J., Fay F., Lawrence J.B. (1993) A three‐dimensional view of precursor messenger RNA metabolism within the mammalian nucleus. Science 259:1330-1335.
Carter K.C., Taneja K.L., Lawrence J.B. (1991) Discrete nuclear domains of poly (A)RNA and their relationship to the functional organization of the nucleus. J. Cell Biol. 115:1191-1202.
Chang L.M.S., Bollum F.J. (1986) Molecular biology of terminal transferase. Crit. Rev. Biochem. 21:27-52.
Cheniclet C., Bendayan M. (1990) Comparative pyrimidine‐ and purine‐specific RNase‐gold labeling on pancreatic acinar cells and isolated hepatocytes. J. Histochem. Cytochem. 38:551-562.
Clevenger C., Epstein A. (1984) Identification of a nuclear protein component of interchromatin granules using a monoclonal antibody and immunogold electron microscopy. Exp. Cell Res. 151:194-207.
Cogliati R., Gautier A. (1973) Mise en évidence de l'ADN et des polysaccharides à l'aide d'un nouveau réactif de “type Schiff.” C. R. Acad. Sci. III 276:3041-3044.
Deng G.R., Wu R. (1983) Terminal transferase: Use in the tailing of DNA and for in vitro mutagenesis. Methods Enzymol. 100:96-116.
Derenzini M., Pession‐Brizzi A., Betts‐Eusebi C., Novello F. (1981) Relationship between the fine structural organization of chromatin and nucleic acid synthesis in regenerating rat hepatocytes. J. Ultrastruct. Res. 75:229-242.
Derenzini M., Hernandez‐Verdun D., Pession A., Novello F. (1983) Structural organization of chromatin in nucleolar organizer regions of nucleoli with a nucleolonema‐like and compact ribonucleoprotein distribution. J. Ultrastruct. Res. 84:161-172.
Derenzini M., Viron A., Puvion E. (1984) Structural organization of rat hepatocyte chromatin as visualized in thin frozen sections selectively stained for DNA. Eur. J. Cell Biol. 33:148-156.
Derenzini M., Thiry M., Goessens G. (1990) Ultrastructural cytocheistry of the mammalian cell nucleolus. J. Histochem. Cyctochem. 38:1237-1256.
Derenzini M., Farabegoli F., Trere D. (1993) Localization of DNA in the fibrillar components of the nucleolus—a cytochemical and morphometric study. J. Histochem. Cytochem. , 829, 336; 41.
Dundr M., Raska I. (1993) Non‐isotopic ultrastructural mapping of transcription sites within the nucleolus. Exp. Cell Res. 208:275-281.
Dupuy‐Coin A.‐M., Kalifat S., Bouteille M. (1972) Nuclear bodies as proteinaceous structures containing ribonucleoproteins. J. Ultrastruct. Res. 38:174-187.
Fakan S. (1976) High‐resolution autoradiography as a tool for the localization of nucleic acid synthesis and distribution in the mammalian cell nucleolus. J. Microsc. 106:159-171.
Fakan S. (1978) High resolution autoradiography studies on chromatin functions. The Cell Nucleus , H. Busch, Academic Press, New York; 5:3-53.
Fakan S. (1986) Structural support for RNA synthesis in the cell nucleus. Methods Achiev. Exp. Pathol. 12:105-140.
Fakan S., Bernhard W. (1971) Localization of rapidly and slowly labelled nuclear RNA as visualized by high resolution autoradiography. Exp. Cell Res. 67:129-141.
Fakan S., Bernhard W. (1973) Nuclear labelling after prolonged 3H‐urdine incorporation as visualized by high resolution autoradiography. Experimental Cell Research 79:431-444.
Fakan S., Hancock R. (1974) Localization of newly‐synthesized DNA in a mammalian cell as visualized by high resolution autoradiography. Exp. Cell Res. 83:95-102.
Fakan S., Modak S. (1973) Localization of DNA in ultrathin tissue sections incubated with terminal deoxynucleotidyl transferase, as visualized by electronmicroscope autoradiography. Exp. Cell Res. 77:95-104.
Fakan S., Nobis P. (1978) Ultrastructural localization of transcription sites and of RNA distribution during the cell cycle of synchronized CHO cells. Exp. Cell Res. 113:327-337.
Fakan S., Puvion E. (1980) The ultrastructural visualization of nucleolar and extranucleolar RNA synthesis and distribution. Int. Rev. Cytol. 65:255-299.
Fakan S., Puvion E., Spohr G. (1976) Localization and characterization of newly synthesized nuclear RNA in isolated rat hepatocytes. Exp. Cell Res. 99:155-164.
Fakan S., Leser G., Martin T. (1984) Ultrastructural distribution of nuclear ribonucleoproteins as visualized by immunocytochemistry on thin sections. J. Cell Biol. 98:358-363.
Fischer D., Weisenberger D., Scheer U. (1991) Assigning functions to nucleolar structures. Chromosoma 101:133-140.
Franke W., Scheer U. (1974) Pathways of nucleocytoplasmic translocation of ribonucleoproteins. Symp. Soc. Exp. Biol. 28:249-282.
Frenster J. (1974) Ultrastructure and function of heterochromatin and euchromatin. The Cell Nucleus , H. Busch, Academic Press, New York; 1:565-580.
Fu X.‐D., Maniatis T. (1990) Factor requiED for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343:437-441.
Goessens G. (1984) Nucleolar structure. Int. Rev. Cytol. 87:107-158.
Granboulan N., Bernhard W. (1961) Cytochimie ultrastructurale. Exploration des structures nucléaires par digestion enzymatique. C.R. Soc. Seances Biol. Fil. 155:1767-1779.
Granboulan N., Granboulan P. (1965) Cytochimie ultrastructurale du nucléole. II. Etude des sites de synthèse du RNA dans le nucléole et le noyau. Exp. Cell Res. 38:604-619.
Hadjiolov A. (1985) The nucleolus and ribosome biogenesis. Cell Biology Monographs , Springer‐Verlag, Wien, New York; 12:1-263.
Hancock R., Boulikas T. (1982) Functional organization in the nucleus. Int. Rev. Cytol. 79:165-214.
Hardin J., Spicer S., Greene W.B. (1969) The paranucleolar structure, accessory body of Cajal, sex chromatin, and related structures in nuclei of rat trigeminal neurons: A cytochemical and ultranstructural study. Anat. Rec. 164:403-432.
Hernandez‐Verdun D., Derenzini M., Bouteille M. (1982) The morphological relationship in electron microscopy between NOR‐silver proteins and intra‐nucleolar chromatin. Chromosoma 85:461-473.
Horisberger M. (1979) Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy. Biol. Cell 36:253-258.
Huang S., Spector D.L. (1992) U1 and U2 SnRNAs are present in nuclear speckles. Proc. Natl. Acad. Sci. U.S.A. 89:305-308.
Hügle B., Scheer U., Franke W. (1985) Ribocharin: A nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit. Cell 41:615-627.
Hutchison N., Weintraub H. (1935) Localization of DNase I‐sensitive sequences to secific regions of interphase nuclei. Cell 43:471-482.
Jacob J., Gillies K., Macleod D., Jones K. (1974) Molecular hybridization of mouse satellite DNA‐complementary RNA in ultrathin sections prepaED for electron microscopy. J. Cell Sci. 14:253-261.
Jansen R., Hurt E., Kern H., Lehtonen H., Carmo‐Fonseca M., Lapeyere B., Tollevey D. (1991) Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast. J. Cell Biol. 113:715-729.
Jimenez‐Garcia G., Seguravaldez M., Ochs R., Echeverria O., Vasquez‐Nin G., Busch H. (1993) Electron microscopic localization of ribosomal DNA in rat liver nucleoli by non‐isotopic in situ hybridization. Exp. Cell Res. 207:220-225.
Jones K. (1970) The chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature 225:912-915.
Jordan G. (1991) Interpreting nucleolus structure: Where are the transcribing genes?. J. Cell Sci. 98:437-442.
Kass S., Tyc K., Steitz J., Sollner‐Webb B. (1990) The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing. Cell 60:897-908.
Kierszenbaum A.L. (1974) RNA synthetic activities of Sertoli cells in the mouse testis. Biol. Reprod. 11:365-376.
Kierszenbaum A.L., Tres L.L. (1974) Nucleolar and perichromosomal RNA synthesis during meiotic prophase in the mouse. J. Cell Biol. 60:39-53.
Lafarga M., Hervas J., Santa‐Cruz M., Villegas J., Crespo D. (1983) The “accessory body” of Cajal in the neuronal nucleus. A light and electron microscopic approach. Anat. Embryol. 166:19-30.
Lafarga M., Andres M., Berciano M., Maquiera E. (1991) Organization of nucleoli and nuclear bodies in osmotically stimulated supraoptic neurons of the rat. J. Comp. Neurol. 308:329-339.
Lafontaine J., Lord A. (1973) An ultrastructural and radioautographic investigation of nucleolonemal component of plant interphase nucleoli. J. Cell Sci. 12:369-383.
Lamond A., Carmo‐Fonseca M. (1993) The coiled body. Trends Cell Biol. 3:198-204.
Lawrence J.B., Singer R. (1986) Intracellular localization of messenger RNAs for cytoskeletal proteins. Cell 45:407-415.
Lawrence J.B., Singer R., Marselle L. (1989) Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization. Cell 57:493-502.
Lehner C., Eppenberger H., Fakan S., Nigg E. (1986) Nuclear substructure antigens monoclonal antibodies against components of nuclear matrix preparations. Exp. Cell Res. 162:205-219.
Leser G., Fakan S., Martin T. (1989) Ultrastructural distribution of ribonucleoprotein complexes during mitosis. SnRNP antigens are contained in mitotic granule clusters. Eur. J. Cell Biol. 50:376-389.
Littau V.C., Allfrey V.G., Frenster J.H., Mirsky A.E. (1964) Active and inactive regions of nuclear chromatin as revealed by electron microscope autoradiography. Proc. Natl. Acad. Sci. U.S.A. 52:93-100.
Manuelidis L. (1990) A view of interphase chromosomes. Science 250:1533-1540.
Martin M., Moreno Diaz de la Espina S., Medina F.J. (1989) Immunolocalization of DNA at nucleolar structural components in onion cells. Chromosoma 98:368-377.
Michelson A., Orkin S. (1982) Characterization of the homopolymer tailing reaction catalysed by terminal deoxynucleotidyl transferase. Implications for the cloning of cDNA. J. Biol. Chem. 257:14773-14782.
Mirre C., Stahl A. (1978) Peripheral RNA synthesis of fibrillar centres in nucleoli of Japanese quail oocytes and somatic cells. J. Ultrastruct. Res. 64:377-387.
Modak S.P., Bollum F.J. (1970) Terminal lens cell differentiation. III. Initiator activity of DNA during nuclear degeneration. Exp. Cell Res. 62:421-432.
Modak S.P., Donnelly G.M., Karasaki S., Hardin C.V., Eddan J., Unakar N. (1973) An enzymatic assay for DNA in epoxy‐tissue sections by light microscope‐autoradiography. Exp. Cell Res. 76:218-222.
Monneron A., Bernhard W. (1969) Fine structural organization of the interphase nucleus in some mammalian cells. J. Ultrastruct. Res. 27:266-288.
Moreno‐Diaz de la Espina S., Medina F., Risueno M. (1980) Correlation of nucleolar activity and nucleolar vacuolation in plant cells. Eur. J. Cell Biol. 22:724-729.
Moreno‐Diaz de la Espina S., Medina F., Risueno M. (1980) Correlation of nucleolar activity and nucleolar vacuolation in plant cells. Eur. J. Cell Biol. 22:724-729.
Moreno‐Diaz de la Espina S., Risueno M., Medina F. (1982) Ultrastructural, cytochemical and autoradiographic characterization of coiled bodies in the plant cell nucleus. Biol. Cell 44:229-238.
Moreno‐Diaz de la Espina S., Sanchez‐Pina M., Risueno M.‐C. (1982) Localization of acid phosphatasic activity, phosphate ions and inorganic cations in plant nuclear coiled bodies. Cell Biol. Int. Rep. 6:266-288.
Moyne G. (1973) Feulgen‐derived techniques for electron microscopical cytochemistry of DNA. J. Ultrastruct. Res. 45:102-123.
Moyne G. (1980) Methods in ultrastructural cytochemistry of the cell nucleus. Prog. Histochem. Cytochem. 13:1-70.
Nash R.E., Puvion E., Bernhard W. (1975) Perichromatin fibrils as components of rapidly labeled extranucleolar RNA. J. Ultrastruct. Res. 53:395-405.
Ochs R., Lischwe M., Spohn W., Busch H. (1985) Fibrillarin: A new protein of the nucleolus identified by autoimmune sera. Biol. Cell 54:123-134.
Padykula H., Clark J. (1981) Nuclear bodies as functional indicators in the target cells of sex steroid hormones. The Cell Nucleus , H. Busch, Academic Press, New York; 9:309-339.
Puvion E., Bernhard W. (1975) Ribonucleoprotein components in liver cell nuclei as visualized by cryoultramicrotomy. J. Cell Biol. 67:200-214.
Puvion E., Moyne G. (1978) Intranuclear migration of newly synthesized extranucleolar ribonucleoproteins. Exp. Cell Res. 115:79-88.
Puvion E., Moyne G. (1981) In situ localization of RNA structures. The Cell Nucleus , H. Busch, Academic Press, New York; 8:59-115.
Puvion E., Viron A., Assens C., Leduc E., Jeanteur P. (1984) Immunocytochemical identification of nuclear structures containing SnRNPs in isolated rat liver cells. J. Ultrastruct. Res. 87:180-189.
Puvion E., Viron A., Xu F. (1984) High resolution autoradiographical detection of RNA in the interchromatin granules of DRB‐treated cells. Exp. Cell Res. 152:357-367.
Puvion‐Dutilleul F., Pichard E., Laithier M., Leduc E. (1987) Effect of dehydrating agents on DNA organization in Herpes viruses. J. Histochem. Cytochem. 35:635-645.
Puvion‐Dutilleul F., Bachellerie J.‐P., Puvion E. (1991) Nucleolar organization of HeLa cells as studied by in situ hybridization. Chromosoma 100:395-409.
Puvion‐Dutilleul F., Mazan S., Nicoloso M., Pichard E., Bachellerie J.‐P., Puvion E. (1992) Alterations of nucleolar ultrastructure and ribosome biogenesis by actinomycin D. Implications for U3 SnRNP function. Eur. J. Cell Biol. 58:149-162.
Rae P., Franke W. (1972) The interphase distribution of satellite DNA containing heterochromatin in mouse nuclei. Chromosoma 39:443-456.
Ramon Y., Cajal S.R. (1903) Um sencillo metodo de coloracion seletiva del reticulo protoplasmico y sus efectos en los diversos organos nerviosos de vertebrados y invertebrados. Trab. Lab. Invest. Biol., (Madrid) 2:129-221.
Raska I., Dundr M. (1993) Compartmentalization of the cell nucleus: Case of the nucleolus. Chromosomes Today , A. T. Sumner, A. C. Chandley, Chapman and Hall, London; 11:101-119.
Raska I., Ochs R., Andrade L., Chan E.K., Burlingame R., Peebles C., Gruol D., Tan E.M. (1990) Association between the nucleolus and the coiled body. J. Struct. Biol. 104:120-127.
Raska I., Andrade L., Ochs R., Chan E.K., Chang C.‐M., Roos G., Tan E.M. (1991) Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies. Exp. Cell Res. 195:27-37.
Recher L., Sykes J., Chan H. (1976) Further studies on the mammalian cell nucleolus. J. Ultrastruct. Res. 56:152-163.
Reddy R., Busch H. (1981) U snRNA's of nuclear snRNP's. The Cell Nucleus , H. Busch, Academic Press, New York; 8:261-306.
Reeder R.H. (1990) rRNA synthesis in the nucleolus. Trends Genet. 6:390-395.
Reimer G., Raska I., Tan E., Scheer U. (1987) Human autoantibodies: Probes for nucleolus structure and function. Virchows Archiv B Cell Pathology Including Molecular Pathology 54:131-143.
Roth J. (1982) The preparation of protein A‐gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra‐thin sections. Histochem. J. 14:791-801.
Roychoudhry R., Jay E., Wu R. (1976) Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 3:863-876.
Savino R., Gerbi S.A. (1990) In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing. EMBO J. 9:2299-2308.
Scheer U., Benavente R. (1990) Functional and dynamic aspects of the mammalian nucleolus. Bioessays 12:14-21.
Scheer U., Raska I. (1987) Immunocytochemical localization of RNA polymerase I in the fibrillar centers of nucleoli. Chromosomes Today , A. Stahl, J. Luciani, A. Wagner‐Capodano, Allen and Unwin, London; 9:284-294.
Scheer U., Rose K. (1984) Localization of RNA polymerase I in interphase cells and mitotic chromosomes by light and electron microscopic immunocytochemistry. Proc. Natl. Acad. Sci. U.S.A. 81:1431-1435.
Scheer U., Thiry M., Goessens G. (1993) Structure, function and assembly of the nucleolus. Trends Cell Biol. 3:236-241.
Schultz M.C. (1990) Three structures associated with the nucleolus in male rat germinal cells: Round body, coiled body and “nubecula” and general presence of round body at male meiosis. Am. J. Anat. 189:11-23.
Seite R., Pebusque M.‐J., Vio‐Cigna M. (1982) Argyrophilic proteins on coiled bodies in sympathetic neurons identified by Ag‐NOR procedure. Biol. Cell 46:97-100.
Simar L.J. (1969) Ultrastructure et constitution des corps nucléaires dans les plasmocytes. Zeitschrift für Zellforschung und Mikroskopische Anatomie 99:235-251.
Singer I.I. (1975) Ultrastructural studies of H‐1 parnovirus replication. II. Induced changes in the deoxyribonucleoprotein and ribonucleoprotein components of human NB cell nuclei. Exp. Cell Res. 95:205-217.
Sippel A.E. (1973) Purification and characterization of adenosine triphosphate: Ribonucleic acid adenyltransferase from Escherichia coli. European Journal of Biochemistry 37:31-40.
Slot J., Genuze H. (1981) Sizing of protein A–colloidal gold probes for immunoelectron microscopy. J. Cell Biol. 90:533-536.
Slot J., Geuze H. (1985) A new method of preparing gold probes for multiple‐labeling cytochemistry. Eur. J. Cell Biol. 38:87-93.
Smetana K., Steele W., Busch H. (1963) A nuclear ribonucleoprotein network. Exp. Cell Res. 31:198-201.
Smith H., Spector D., Woodcock C., Ochs R., Bhorjee J. (1985) Alterations in chromatin conformation are accompanied by reorganization of nonchromatin domains that contain U‐snRNP protein p28 and nuclear protein p107. J. Cell Biol. 101:560-567.
Sollner‐Webb B., Mougey E.B. (1991) News from the nucleolus: rRNA gene expression. Trends Biochem. Sci. 16:58-62.
Sommerville J. (1986) Nucleolar structure and ribosome biogenesis. Trends Biochem. Sci. 11:438-442.
Spector D. (1990) Higher order nuclear organization: Three‐dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. U.S.A. 87:147-151.
Spector D., Schrier W., Busch H. (1983) Immunoelectron microscopic localization of SnRNPs. Biol. Cell 49:1-10.
Spector D., Fu X.‐D., Maniatis T. (1991) Associations between distinct pre‐mRNA splicing components and the cell nucleus. EMBO J. 10:3467-3481.
Stierhof Y.‐D., Schwarz H. (1988) Immunoelectronmicroscopy on cryosections section permeability to specific antibodies, protein A‐gold complexes and ferritin conjugated IgGs. Inst. Phys. Conf. Ser. number 93 3:519-520.
Stierhof Y.‐D., Schwarz H., Frank H. (1986) Transverse sectioning of plastic‐embedded immunolabeled cryosections: Morphology and permeability to protein A–colloidal gold complexes. J. Ultra struct. Res. 97:187-196.
Swift H. (1962) Nucleoprotein localization in electron micrograghs: Metal binding and radioautography. Symp. Int. Soc. Cell Biol. 2:21.
Swift H. (1963) Cytochemical studies on nuclear fine structure. Exp. Cell Res. 9:54-67.
Thiry M. (1988) Immunoelectron microscope localization of bromodeoxyuridine incorporated into DNA of Ehrlich tumor cell nucleoli. Exp. Cell Res. 179:204-213.
Thiry M. (1991) DNase I–sensitive sites within the nuclear architecture visualized by immunoelectron microscopy. DNA Cell Biol. 10:169-180.
Thiry M. (1991) In situ nick‐translation at the electron microscope level: A tool for studying the location of Dnase I–sensitive regions within the cell. J. Cytochem. Histochem. 39:871-874.
Thiry M. (1992) Ultrastructural detection of DNA within the nucleolus by sensitive molecular immunocytochemistry. Exp. Cell Res. 200:135-144.
Thiry M. (1992) Highly sensitive immunodetection of DNA on sections with exogeneous terminal deoxunucleotidyl transferase and non‐isotopic nucleotide analogues. J. Histochem. Cytochem. 40:411-419.
Thiry M. (1992) New data concerning the functional organization of the mammalian cell nucleolus: Detection of RNA and rRNA by in situ molecular immunocytochemistry. Nucleic Acids Res. 20:6195-6200.
Thiry M. (1993) Ultrastructural distribution of DNA and RNA within the nucleolus of human Sertoli cells as seen by molecular immunocytochemistry. J. Cell Sci. 105:33-39.
Thiry M. (1993) Immunodetection of RNA on ultrathin sections incubated with polyadenylate nucleotidyl transferase. J. Histochem. Cytochem. 41:657-665.
Thiry M. (1993) Diffential location of nucleic acids within interchromatin granule clusters. Eur. J. Cell Biol. 62:259-269.
Thiry M., Goessens G. (1986) Ultrastructural study of the relationships between the various nucleolar components in Ehrlich tumour and HEp‐2 cell nucleoli after acetylation. Exp. Cell Res. 164:232-242.
Thiry M., Goessens G. (1991) Distinguishing the sites of pre‐rRNA synthesis and accumulation in Ehrlich tumor cell nucleoli. J. Cell Sci. 99:759-767.
Thiry M., Goessens G. (1992) Location of DNA within the nucleolus of rat oocytes during the early stages of follicular growth. Int. J. Dev. Biol. 36:139-142.
Thiry M., Goessens G. (1992) Where, within the nucleolus, are the rRNA genes located?. Exp. Cell Res. 200:1-4.
Thiry M., Thiry‐Blaise L. (1989) In situ hybridization at the electron microscope level: An improved method for precise localization of ribosomal DNA and RNA. Eur. J. Cell Biol. 50:235-243.
Thiry M., Thiry‐Blaise L. (1991) Locating transcribed and non‐transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy. Nucleic Acids Res. 19:11-15.
Thiry M., Scheer U., Goessens G. (1988) Localization of DNA within Ehrlich tumour cell nucleoli by immunoelectron microscopy. Biol. Cell 63:27-34.
Thiry M., Scheer U., Goessens G. (1991) Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level. Electron Microsc. Rev. 4:85-110.
Thiry M., Schoonbroodt S., Goessens G. (1991) Cytochemical distinction of various nucleolar components in insect cells. Biol. Cell 72:133-140.
Thiry M., Ploton D., Ménager M., Goessens G. (1993) Ultrastructural distribution of DNA within the nucleolus of various animal cell lines or tissues revealed by terminal deoxynucleotidyl transferase. Cell Tissue Res. 271:33-45.
Tokuyasu K., Madden S., Zeldis L. (1968) Fine structural alterations of interphase nuclei of lymphocytes stimulated to growth activity in vitro. J. Cell Biol. 39:630-660.
Turner B.M., Franchi L. (1987) Identification of proteins antigens associated with the nuclear matrix and with clusters of interchromatin granules in both interphase and mitotic cells. J. Cell Sci. 87:269-282.
Tyc K., Steitz J.A. (1989) U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus. EMBO J. 8:3113-3119.
Unuma T., Arendell J., Busch H. (1968) High resolution autoradiographic studies of the uptake of 3H‐5‐Urdine into condensed and dispersed chromatin of nuclei and granular and fibrillar components of nucleoli of Novikoff hepatoma ascites cells. Exp. Cell Res. 52:429-438.
Vagner‐Capodano A.M., Stahl A. (1980) Relationship of chromatin to nucleolar fibrillar centre revealed by action of actinomycin D. Biol. Cell 37:293-296.
Vandelaer M., Thiry M., Goessens G. (1993) Ultrastructural distribution of DNA within the ring‐shaped nucleolus of human resting T lymphocytes. Exp. Cell Res. 205:430-432.
Vazquez Nin G., Flores E., Echeverria O., Merkert H., Wettstein R., Benavente R. (1993) Immunocytochemical localization of DNA in synaptonemal complexes of rat and mouse spermatocytes, and of chick oocytes. Chromosoma 102:457-463.
Visa N., Puvion‐Dutilleul F., Bachellerie J.‐P., Puvion E. (1993) Intranuclear distribution of U1 and U2 SnRNAs visualized by high resolution in situ hybridization: Revelation of a novel compartment containing U1 but not U2 SnRNA in HeLa cells. Eur. J. Cell Biol. 60:308-321.
Wachtler F., Mosgöller W., Schwarzacher H. (1990) Electron microscopic in situ hybridization and autoradiography: Localization and transcription of rDNA in human lymphocyte nucleoli. Exp. Cell Res. 187:346-348.
Wachtler F., Schofer C., Mosgoller W., Weipoltshammer K., Schwarzacher H.G., Guichaoua M., Hartung M., Stahl A., Bergé‐Lefranc J.L., Gonzalez I., Sylvester J. (1992) Human ribosomal RNA gene repeats are localized in the dense fibrillar component of nucleoli: Light and electron microscopic in situ hybridization in human Sertoli cells. Exp. Cell Res. 198:135-143.
Warner J.R. (1990) The nucleolus and ribosome formation. Curr. Opin. Cell Biol. 2:521-527.
Watson M.L. (1962) Quantitative methods in biology. Practical Methods in Electron Microscopy , A. Glauert, American Elsevier, North‐Holland; 6:1-216.
Xing Y., Johnson C., Dobner P., Lawrence J.B. (1993) Higher level organization of individual gene transcription and RNA splicing. Science 259:1326-1330.
Zamore P., Green M.R. (1991) Biochemical characterization of U2SnRNP auxiliary factor: An essential pre‐mRNA splicing factor with a novel intranuclear distribution. EMBO J. 10:207-214.
Zareba‐Kowalska A. (1989) Cytochemical observations of the coiled bodies in neurons of rat sympathetic ganglia. Histochemistry 91:251-256.