Escherichia coli; Staphylococcus aureus; Streptococcus uberis; coagulase-negative staphylococci; dairy cows; growth inhibition; mastitis; Immunology and Allergy; Molecular Biology; Immunology and Microbiology (all); Microbiology (medical); Infectious Diseases; General Immunology and Microbiology
Abstract :
[en] In this paper, we describe a new quantitative method to evaluate and quantify in vitro growth inhibition of mastitis-related bacteria. Colony-forming units of Staphylococcus (S.) aureus (n = 10), Escherichia (E.) coli (n = 10), and Streptococcus (S.) uberis (n = 10) were quantified after their growth on top of layers of trypticase soy agar (TSA) containing six different concentrations (varying from 102 to 107 CFU/mL) of bovine non-aureus staphylococci (NAS), i.e., S. chromogenes (n = 3) and S. simulans (n = 3) isolates. Growth inhibition of the mastitis-related major bacterial pathogens, including E. coli, was confirmed by all NAS, an effect that varied highly among NAS isolates and was not evident from the semiquantitative method with which the new method was compared. By subsequent application of the new method on a larger set of 14 bovine NAS isolates, we observed that S. simulans and NAS originating from teat apices (especially S. epidermidis) required lower concentrations to inhibit both methicillin-sensitive (MSSA) (n = 5) and methicillin-resistant S. aureus (MRSA) isolates (n = 5) originating from milk. Therefore, the new assay is a promising tool to precisely quantify the intra- and inter-species differences in growth inhibition between NAS.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Toledo-Silva, Bruno ; M-Team and Mastitis and Milk Quality Research Unit, Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Beuckelaere, Lisa ; M-Team and Mastitis and Milk Quality Research Unit, Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
De Visscher, Anneleen; Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science, Agricultural Engineering, 9820 Merelbeke, Belgium
Geeroms, Chloë; M-Team and Mastitis and Milk Quality Research Unit, Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium ; Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Meyer, Evelyne; Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Piepers, Sofie; M-Team and Mastitis and Milk Quality Research Unit, Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Thiry, Damien ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Haesebrouck, Freddy ; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
De Vliegher, Sarne ; M-Team and Mastitis and Milk Quality Research Unit, Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Language :
English
Title :
Novel Quantitative Assay to Describe In Vitro Bovine Mastitis Bacterial Pathogen Inhibition by Non-aureus Staphylococci.
FWO - Flemish Research Foundation [BE] CAPES - Coordenação de Aperfeicoamento de Pessoal de Nível Superior [BR] VLAIO - Flanders Innovation and Entrepreneurship [BE] Elanco [US-IN] [US-IN]
Funding text :
Funding: This research was funded by Flanders Innovation & Entrepreneurship (VLAIO), Elanco Animal Health (Greenfield, IN, USA), and the Brazilian research funding agency CAPES. The MALDI-TOF mass spectrometer was funded by the Research Foundation Flanders (FWO-Flanders) as Hercules project G0H2516N (AUGE/15/05).
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