GM1 ganglioside; MD simulations; Model membrane; Neutron reflectometry; SAXS; Glycolipids; Glycosphingolipids; Lipid Bilayers; Sugars; rhamnolipid hemolysin, Pseudomonas aeruginosa; Cell Membrane/chemistry; Lipid Bilayers/chemistry; Glycosphingolipids/analysis; Molecular Dynamics Simulation; Carbohydrate-carbohydrate interactions; Dirhamnolipid; MD simulation; Membrane surface; Model membranes; Rhamnolipids; Cell Membrane; Electronic, Optical and Magnetic Materials; Biomaterials; Surfaces, Coatings and Films; Colloid and Surface Chemistry
Abstract :
[en] Rhamnolipids (RLs) are among the most important biosurfactants produced by microorganisms, and have been widely investigated because of their multiple biological activities. Their action appears to depend on their structural interference with lipid membranes, therefore several studies have been performed to investigate this aspect. We studied by X-ray scattering, neutron reflectometry and molecular dynamic simulations the insertion of dirhamnolipid (diRL), the most abundant RL, in model cellular membranes made of phospholipids and glycosphingolipids. In our model systems the affinity of diRL to the membrane is highly promoted by the presence of the glycosphingolipids and molecular dynamics simulations unveil that this evidence is related to sugar-sugar attractive interactions at the membrane surface. Our results improve the understanding of the plethora of activities associated with RLs, also opening new perspectives in their selective use for pharmaceutical and cosmetics formulations. Additionally, they shed light on the still debated role of carbohydrate-carbohydrate interactions as driving force for molecular contacts at membrane surface.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rondelli, Valeria; Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Italy. Electronic address: valeria.rondelli@unimi.it
Mollica, Luca ; Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Italy
Koutsioubas, Alexandros ; Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, Forschungszentrum Jülich GmbH, Garching, Germany
Nasir, Nail; Laboratoire de Biophysique Moléculaire aux Interfaces, Structure Fédérative de Recherche Condorcet, TERRA Research Center, Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgium, Astel Medica SA., Tinlot, Belgium
Trapp, Marcus; Gesellschaft für Anlagen- und Reaktorsicherheit (GRS) gGmbH, Schwertnergasse 1, 50667 Köln, Germany, Helmholtz-Zentrum Berlin für Materialien und Energie, Institute for Soft Matter and Functional Materials, Hahn-Meitner-Platz 1, 14109 Berlin, Germany
Deboever, Estelle ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Brocca, Paola; Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Italy
Deleu, Magali ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Language :
English
Title :
Carbohydrate-carbohydrate interaction drives the preferential insertion of dirhamnolipid into glycosphingolipid enriched membranes.
The authors thank the Belgian Fund for Scientific Research (F.R.S.-FNRS) for its financial support via the CDR Project J.0086.18 and the University of Liège for its support via the ARC-FIELD project 13/17-10.This project has received funding from the European Union's Horizon. 2020 research and innovation programme under grant agreement No 731019. (EUSMI). This research was partly funded by the ?Medical Biotechnology and Translational Medicine Department? of the ?Universit? degli Studi di Milano?, grant number ?PSR2018? and 'PSR2020' to V.R. The authors thank the Belgian Fund for Scientific Research (F.R.S.-FNRS) for its financial support via the CDR Project J.0086.18 and the University of Li?ge for its support via the ARC-FIELD project 13/17-10. The authors thank the instrument teams of MARIA, J?lich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ, Garching, Germany) of V18 reflectometer at HZB (Berlin, Germany) and of ID02 beamline at the European Synchrotron Radiation Facility (ESRF, Grenoble, France) for support and the facilities for allocation of beamtime. M.D. thanks the F.R.S.-FNRS for her position as Senior Research Associate.(EUSMI). This research was partly funded by the ‘Medical Biotechnology and Translational Medicine Department’ of the ‘Università degli Studi di Milano’, grant number ‘PSR2018’ and 'PSR2020' to V.R.2020 research and innovation programme under grant agreement No 731019.This project has received funding from the European Union’s Horizon.
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