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Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.
Thiry, Marc; Scheer, U.; Goessens, Guy
1991In Electron Microscopy Reviews, 4 (1), p. 85-110
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Keywords :
Animals; Cell Nucleolus/metabolism/ultrastructure; Chromatin/metabolism/ultrastructure; DNA/metabolism; Histones/metabolism; Humans; Immunohistochemistry; Microscopy, Electron; Nucleic Acid Hybridization; RNA, Ribosomal/genetics/metabolism
Abstract :
[en] Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure. Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA topoisomerase I are located together. Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Scheer, U.
Goessens, Guy ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.
Publication date :
1991
Journal title :
Electron Microscopy Reviews
ISSN :
0892-0354
Publisher :
Pergamon Press, Elmsford, United States - New York
Volume :
4
Issue :
1
Pages :
85-110
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

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