Reference : Location of rRNA gene transcription sites within Ehrlich tumor cell nucleoli.
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Location of rRNA gene transcription sites within Ehrlich tumor cell nucleoli.
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Bulletin de l'Association des Anatomistes
Association des Anatomistes
Yes (verified by ORBi)
[en] Animals ; Carcinoma, Ehrlich Tumor/genetics/ultrastructure ; Cell Nucleolus/ultrastructure ; DNA, Ribosomal/analysis/genetics/ultrastructure ; RNA, Neoplasm/genetics/ultrastructure ; RNA, Ribosomal/genetics/ultrastructure ; Transcription, Genetic/genetics
[en] The nucleolus is the morphological visualization of ribosome biogenesis. The spatial distribution of nucleolar DNA has been investigated in Ehrlich tumor cells by various immunogold labelling techniques. The DNase 1-sensitive regions within the nucleolus have been identified by in situ nick-translation at the ultrastructural level. The precise nucleolar location of rDNA and rRNA has been further specified by in situ hybridization and electron microscopy. Our results indicate that the fibrillar centers are the sole nucleolar structures where DNase 1-hypersensitive sequences, rDNA and rRNA are located together. These findings strongly suggest that rRNA gene transcription takes place within the confines of the fibrillar center, probably close to the boundary regions to the surrounding dense fibrillar component.

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