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Localization of DNA within Ehrlich tumour cell nucleoli by immunoelectron microscopy.
Thiry, Marc; Scheer, U.; Goessens, Guy
1988In Biology of the Cell, 63 (1), p. 27-34
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Keywords :
Animals; Carcinoma, Ehrlich Tumor/ultrastructure; Cell Nucleolus/ultrastructure; DNA/ultrastructure; Immunohistochemistry; Mice; Microscopy, Electron/methods; Tumor Cells, Cultured
Abstract :
[en] The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach involving a monoclonal antibody directed against double- and single-stranded DNA. Immunolabelling was performed either before or after the embedding process. The postembedding labelling method allows better ultrastructural preservation than the preembedding labelling method. In particular, the various nucleolar components are well preserved and identifiable. In the nucleolus, labelling is particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations, which penetrate the nucleolar body and often terminate at the fibrillar centres. In addition, aggregates of gold particles are found in the fibrillar centres, preferentially towards the peripheral regions. By contrast, the dense fibrillar component is completely devoid of labelling. The results seem to indicate that DNA containing the rDNA genes is located in the fibrillar centres, with a preference for the peripheral regions. This finding suggests that transcription of the rDNA genes should occur within the confines of the fibrillar centre, probably close to the boundary region of the surrounding dense fibrillar component. The results are discussed in the light of present knowledge of the functional organization of the nucleolus.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Scheer, U.
Goessens, Guy ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Localization of DNA within Ehrlich tumour cell nucleoli by immunoelectron microscopy.
Publication date :
1988
Journal title :
Biology of the Cell
ISSN :
0248-4900
eISSN :
1768-322X
Publisher :
Portland Press, Colchester, United Kingdom
Volume :
63
Issue :
1
Pages :
27-34
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

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