The influence of thyroid state on hypothalamic AMP-activated protein kinase pathways in broilers.

AMPK; Broiler Chicks; Feed Intake; Hypothalamus; Thyroid Hormones; AMP-Activated Protein Kinases; Animals; Eating; Energy Metabolism; Homeostasis; Hypothalamus/enzymology; Thyroid Gland/metabolism; Thyroid Gland/physiopathology; AMP-Activated Protein Kinases/metabolism; Chickens/metabolism; Chickens; Thyroid Gland; Animal Science and Zoology; Endocrinology

Abstract :

[en] To investigate whether there are important interactions in play in broilers between thyroid hormones and the central regulation of energy homeostasis through AMP-activated protein kinase (AMPK), we induced a functional hyperthyroid and hypothyroid state in broiler chicks, and quantified systemic and hypothalamic AMPK related gene expression and related protein. Thyroid state was manipulated through dietary supplementation of triiodothyronine (T3) or methimazole (MMI) for 7 days. A hypothalamic AMPK suppressor, 0.1% α-lipoic acid (α-LA) was used to assess the effects of the T3 and MMI feed formulations on the AMPK pathways. Feed intake and body weight were reduced in both hypothyroid and hyperthyroid conditions. In hyperthyroid conditions (T3 supplementation) expression of the AMPKα1 subunit increased, while in hypothyroid conditions (MMI supplementation) active phosphorylated AMPK levels in the hypothalamus dropped, but gene expression of the AMPKα1 and α2 subunit increased. For FAS and ACC (involved in fatty acid metabolism), and CRH, TRH and CNR1 (anorexigenic neuropeptides stimulating energy expenditure) there were indications that their regulation in response to thyroid state might be modulated through AMPK pathways. Our results indicate that the expression of hypothalamic AMPK as well as that of several other genes from AMPK pathways are involved in thyroid-hormone-induced changes in appetite, albeit differently according to thyroid state.

Disciplines :

Agriculture & agronomy

Author, co-author :

Lamberigts, C; Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium

Wang, Y; Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium

Dierckx, T; Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, Herestraat 49 box 1030, 3000 Leuven, Belgium

Buys, N; Laboratory of Livestock Genetics, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium

Everaert, Nadia ; Université de Liège - ULiège > Département GxABT

Buyse, J; Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium. Electronic address: johan.buyse@kuleuven.be

Language :

English

Title :

The influence of thyroid state on hypothalamic AMP-activated protein kinase pathways in broilers.

Publication date :

2021

Journal title :

General and Comparative Endocrinology

ISSN :

0016-6480

eISSN :

1095-6840

Publisher :

Academic Press Inc., United States

Volume :

311

Pages :

113838

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE]

Funding text :

This work was supported by the ‘ Fonds Wetenschappelijk Onderzoek–Vlaanderen ’ (FWO, project number GOA7415N ). The authors are very grateful to Daniel Vermeulen, André Respen and Dries Robijns for their technical assistance.

Available on ORBi :

since 20 June 2022

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