[en] Glandular trichomes of tobacco (Nicotiana tabacum) produce blends of acylsucroses contributing to the defence against pathogens and herbivorous insects. However, the mechanism of acylsugar assembly remains to be elucidated. We report on the isolation and characterization of two trichome-specific and endoplasmic-reticulum localized acylsugar acyltransferases, NtASAT1 and NtASAT2. They sequentially catalyse two additive steps of acyl donors to sucrose producing bi-acylsucrose. Knocking-out of NtASAT1 or NtASAT2 results in deficiency of acylsucrose, however, there was no effect on acylsugar accumulation in NtASAT1 or NtASAT2 overexpressing plants. Genomic and acylsugar profile analysis revealed that NtASATs are originated from the T subgenome, which is derived from diploid ancestor N. tomentosiformis producing acylsugars. These results revealed that NtASAT1 and NtASAT2 are enzymes involved in the acylsugar assembly in tobacco, providing a new approach and target genes for improving crop resistance against pathogens and insects.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Chang, Aixia ; Université de Liège - ULiège > TERRA Research Centre ; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao
Hu, Zhongyi ; Jiangxi Food Inspection and Testing Research Institute, Nanchang, China.
Chen, Biao; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Vanderschuren, Hervé ; Université de Liège - ULiège > Département GxABT > Plant Sciences ; Tropical Crop Improvement Lab, Department of Biosystems, KU Leuven, Heverlee
Chen, Ming; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Qu, Yafang; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Yu, Weisong; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Li, Yangyang ; Hunan Tobacco Research Institute, Changsha, China.
Sun, Huiqing; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Cao, Jianmin; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Vasudevan, Kumar ; Université de Liège - ULiège > Département GxABT > Plant Sciences
Li, Chenying; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Cao, Yanan; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
Zhang, Jianye; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou,
Shen, Yeming ; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou
Yang, Aiguo ; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao
Wang, Yuanying; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao,
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