[en] Thermosensation is crucial for the survival of any organism. In animals, changes in brain temperature are detected via sensory neurons, their cell bodies are located in the trigeminal ganglia. Transient receptor potential (TRP) ion channels are the largest temperature sensing family. In mammals, 11 thermoTRPs are known, as in poultry, there are only three. This research further elucidates TRP mRNA expression in the brain of broiler embryo's. Three incubation treatments were conducted on 400 eggs each: the control (C) at 37.6 °C; T1 deviating from C by providing a + 1 °C heat stimuli during embryonic day (ED) 15-20 for 8 h a day; and T2, imposing a + 2 °C heat stimuli. After each heat stimuli, 12 eggs per treatment were taken for blood sampling from the chorioallantoic membrane and brain harvesting. Incubation parameters such has residual yolk (free embryonic) weight, chick quality and hatch percentage were collected. After primer optimization, 22 target genes (13 TRPs and 9 non-TRPs) were measured on mRNA of the brain using a nanofluidic biochip (Fluidigm Corporation). Four target genes (ANO2, TRPV1, SCN5A, TRAAK) have a significant treatment effect - independent of ED. Another four (TRPM8, TRPA1, TRPM2, TRPC3) have a significant treatment effect visible on one or more ED. Heat sensitive channels were increased in T2 and to a lesser degree in T1, which could be part of an acclimatisation process resulting in later life heat tolerance by increased heat sensitivity. T2, however, resulted in a lower hatch weight, quality and hatchability. No hormonal differences were detected.
Disciplines :
Agriculture & agronomy
Author, co-author :
Verlinden, Sara ; Université de Liège - ULiège > TERRA Research Centre ; Department of Biosystems, Divison Animal and human health engineering, M3-BIORES, KU Leuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Norton, Tomas; Department of Biosystems, Divison Animal and human health engineering, M3-BIORES, KU Leuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Larsen, Mona Lilian Vestbjerg; Department of Biosystems, Divison Animal and human health engineering, M3-BIORES, KU Leuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium, Department of Animal Science, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark
Youssef, Ali; Department of Biosystems, Divison Animal and human health engineering, M3-BIORES, KU Leuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium, Adaptation Physiology Group, Wageningen University & Research, P.O. Box 338, 6700, AH, Wageningen
Everaert, Nadia ; Université de Liège - ULiège > Département GxABT ; Department of Biosystems, Divison Animal and Human Health engineering, NAMES, KU Leuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium. Electronic address: nadia.everaert@kuleuven.be
Language :
English
Title :
Influence of temperature during incubation on the mRNA levels of temperature sensitive ion channels in the brain of broiler chicken embryos.
Publication date :
2022
Journal title :
Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology
This work was supported VLAIO (Vlaams agentschap innoveren en ondernemen, number HBC.2017.1001 ). This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
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