Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Cell Biology; Microbiology; Neuroscience; Animals; Cell Movement; Cerebral Cortex; Interneurons; Mice; Microdissection; Tissue Culture Techniques; Biochemistry, Genetics and Molecular Biology (all); Neuroscience (all); Immunology and Microbiology (all); Medicine (all); General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Neuroscience

Abstract :

[en] Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Lepiemme, Fanny ^✱; Université de Liège - ULiège > GIGA

Silva, Carla G. ^✱; GIGA-Stem Cells and GIGA-Neurosciences, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, CHU Sart-Tilman, Liège, Belgium

Nguyen, Laurent ^✱; Université de Liège - ULiège > GIGA > GIGA Stem Cells

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Publication date :

18 June 2021

Journal title :

STAR Protocols

eISSN :

2666-1667

Publisher :

Cell Press

Volume :

Issue :

Pages :

100467

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S266616672100174X?httpAccept=text/xml

Name of the research project :

ERANET Neuron STEM-MCD and NeuroTalk

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Fonds Léon Fredericq [BE]
FMRE - Fondation Médicale Reine Elisabeth [BE]
Fondation Pierre et Simone Clerdent [BE]
BELSPO - Belgian Science Policy Office [BE]

Funding text :

We thank Alexandre Hego and Sandra Ormenese (GIGA Cell Imaging Platform) for their advices. The graphical abstract was done with BioRender software. The work in the Nguyen Laboratory is supported by the F.R.S.-F.N.R.S. (Synet; EOS 0019118F-RG36 ), the Fonds Léon Fredericq , the Fondation Médicale Reine Elisabeth , the Fondation Simone et Pierre Clerdent , the Belgian Science Policy (IAP-VII network P7/20), and the ERANET Neuron STEM-MCD and NeuroTalk .We thank Alexandre Hego and Sandra Ormenese (GIGA Cell Imaging Platform) for their advices. The graphical abstract was done with BioRender software. The work in the Nguyen Laboratory is supported by the F.R.S.-F.N.R.S. (Synet; EOS 0019118F-RG36), the Fonds L?on Fredericq, the Fondation M?dicale Reine Elisabeth, the Fondation Simone et Pierre Clerdent, the Belgian Science Policy (IAP-VII network P7/20), and the ERANET Neuron STEM-MCD and NeuroTalk. This protocol relies on experiments done by F.L. and C.G.S. and has been written by F.L. C.G.S. and L.N. The authors declare no competing interests.

Available on ORBi :

since 06 June 2022

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Bibliography

Bellion, A., Baudoin, J.P., Alvarez, C., Bornens, M., Métin, C., Nucleokinesis in tangentially migrating neurons comprises two alternating phases: Forward migration of the Golgi/centrosome associated with centrosome splitting and myosin contraction at the rear. J. Neurosci. 25 (2005), 5691–5699.
Godin, J.D., Thomas, N., Laguesse, S., Malinouskaya, L., Close, P., Malaise, O., Purnelle, A., Raineteau, O., Campbell, K., Fero, M., et al. p27kip1 is a microtubule-associated protein that promotes microtubule polymerization during neuron migration. Dev. Cell 23 (2012), 729–744.
Meijering, E., Dzyubachyk, O., Smal, I., Methods for cell and particle tracking. Methods Enzymol. 504 (2012), 183–200.
Nadarajah, B., Brunstrom, J.E., Grutzendler, J., Wong, R.O.L., Pearlman, A.L., Two modes of radial migration in early development of the cerebral cortex. Nat. Neurosci. 4 (2001), 143–150.
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. Fiji: An open-source platform for biological-image analysis. Nat. Methods 9 (2012), 676–682.
Silva, C.G., Peyre, E., Adhikari, M.H., Tielens, S., Tanco, S., Van Damme, P., Magno, L., Krusy, N., Agirman, G., Magiera, M.M., et al. Cell-intrinsic control of interneuron migration drives cortical morphogenesis. Cell 172 (2018), 1063–1078.
Stenman, J., Toresson, H., Campbell, K., Identification of two distinct progenitor populations in the lateral ganglionic eminence: Implications for striatal and olfactory bulb neurogenesis. J. Neurosci. 23 (2003), 167–174.