Keywords :
Cell Biology; Microbiology; Neuroscience; Animals; Cell Movement; Cerebral Cortex; Interneurons; Mice; Microdissection; Tissue Culture Techniques; Biochemistry, Genetics and Molecular Biology (all); Neuroscience (all); Immunology and Microbiology (all); Medicine (all); General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Neuroscience
Abstract :
[en] Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).
Silva, Carla G. ✱; GIGA-Stem Cells and GIGA-Neurosciences, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, CHU Sart-Tilman, Liège, Belgium
✱ These authors have contributed equally to this work.
Funding text :
We thank Alexandre Hego and Sandra Ormenese (GIGA Cell Imaging Platform) for their advices. The graphical abstract was done with BioRender software. The work in the Nguyen Laboratory is supported by the F.R.S.-F.N.R.S. (Synet; EOS 0019118F-RG36 ), the Fonds Léon Fredericq , the Fondation Médicale Reine Elisabeth , the Fondation Simone et Pierre Clerdent , the Belgian Science Policy (IAP-VII network P7/20), and the ERANET Neuron STEM-MCD and NeuroTalk .We thank Alexandre Hego and Sandra Ormenese (GIGA Cell Imaging Platform) for their advices. The graphical abstract was done with BioRender software. The work in the Nguyen Laboratory is supported by the F.R.S.-F.N.R.S. (Synet; EOS 0019118F-RG36), the Fonds L?on Fredericq, the Fondation M?dicale Reine Elisabeth, the Fondation Simone et Pierre Clerdent, the Belgian Science Policy (IAP-VII network P7/20), and the ERANET Neuron STEM-MCD and NeuroTalk. This protocol relies on experiments done by F.L. and C.G.S. and has been written by F.L. C.G.S. and L.N. The authors declare no competing interests.
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