Article (Scientific journals)
Tetrahydrofolate Modulates Floral Transition through Epigenetic Silencing.
Wang, Lei; Kong, Dongdong; Lv, Qiang et al.
2017In Plant Physiology, 174 (2), p. 1274-1284
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Keywords :
Tetrahydrofolates; Polyglutamic Acid; Arabidopsis/genetics; Arabidopsis/physiology; DNA Methylation/genetics; Flowers/genetics; Gene Expression Profiling; Genome, Plant; Arabidopsis; DNA Methylation; Epigenesis, Genetic; Flowers; Gene Expression Regulation, Plant; Gene Silencing; Physiology; Genetics; Plant Science
Abstract :
[en] Folates, termed from tetrahydrofolate (THF) and its derivatives, function as coenzymes in one-carbon transfer reactions and play a central role in synthesis of nucleotides and amino acids. Dysfunction of cellular folate metabolism leads to serious defects in plant development; however, the molecular mechanisms of folate-mediated cellular modifications and physiological responses in plants are still largely unclear. Here, we reported that THF controls flowering time by adjusting DNA methylation-regulated gene expression in Arabidopsis (Arabidopsis thaliana). Wild-type seedlings supplied with THF as well as the high endogenous THF content mutant dihydrofolate synthetase folypoly-Glu synthetase homolog B exhibited significant up-regulation of the flowering repressor of Flowering Wageningen and thereby delaying floral transition in a dose-dependent manner. Genome-wide transcripts and DNA methylation profiling revealed that THF reduces DNA methylation so as to manipulate gene expression activity. Moreover, in accompaniment with elevated cellular ratios between monoglutamylated and polyglutamylated folates under increased THF levels, the content of S-adenosylhomo-Cys, a competitive inhibitor of methyltransferases, was obviously higher, indicating that enhanced THF accumulation may disturb cellular homeostasis of the concerted reactions between folate polyglutamylation and folate-dependent DNA methylation. In addition, we found that the loss-of-function mutant of CG DNA methyltransferase MET1 displayed much less responsiveness to THF-associated flowering time alteration. Taken together, our studies revealed a novel regulatory role of THF on epigenetic silencing, which will shed lights on the understanding of interrelations in folate homeostasis, epigenetic variation, and flowering control in plants.
Disciplines :
Genetics & genetic processes
Biochemistry, biophysics & molecular biology
Author, co-author :
Wang, Lei ;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Kong, Dongdong ;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China, ddkong@cnu.edu.cn ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W.), ddkong@cnu.edu.cn ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ddkong@cnu.edu.cn ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H.) ddkong@cnu.edu.cn
Lv, Qiang;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Niu, Guoqi;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Han, Tingting;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Zhao, Xuanchao;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Meng, Shulin;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Cheng, Qian;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Guo, Shouchun;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Du, Jing;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Wu, Zili;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Wang, Jinzheng;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Bao, Fang;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Hu, Yong;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Pan, Xiaojun;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Xia, Jinchan;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Yuan, Dong;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Han, Lida;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Lian, Tong  ;  Université de Liège - ULiège > TERRA Research Centre ; College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Zhang, Chunyi;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
Wang, Haiyang ;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
He, Xin-Jian ;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
He, Yi-Kun ;  College of Life Sciences (L.W., D.K., Q.L., G.N., T.H., X.Z., S.M., Q.C., S.G., J.D., J.W., F.B., Y.H., X.P., J.X., Y.-k.H.) and Department of Chemistry (D.K.), Capital Normal University, Beijing 100048, China ; College of Life Science and Food Engineering, Yibin University, Yibin 644000, China (Z.W ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China (D.Y., L.H., T.L., C.Z., H.W.), and ; National Institute of Biological Sciences, Beijing 102206, China (X.-J.H
More authors (13 more) Less
Language :
English
Title :
Tetrahydrofolate Modulates Floral Transition through Epigenetic Silencing.
Publication date :
June 2017
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
American Society of Plant Biologists, United States
Volume :
174
Issue :
2
Pages :
1274-1284
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Ministry of Science and Technology of China
National Natural Science Foundation of China (NSFC)
Funding text :
This work was supported by grants from the Ministry of Science and Technology of China (2013CB967300) and National Natural Science Foundation of China (31530006) to Y.-k.H. (Capital Normal University, China).
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since 26 May 2022

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