[en] Folates are essential micronutrients for human health. To determine the total folate content, the extraction and quantification of seven monoglutamate folate derivatives in cereals (maize, rice, and wheat) were optimised and validated in this study. Di-enzyme treatment with α-amylase and rat conjugase was proved ideal for folate extraction from the cereal grains. The quantification method by liquid chromatography-tandem mass spectrometry was validated based on its matrix effect, linearity, sensitivity, recovery, inter-day and intra-day precision. The limits of detection and quantification of folate derivatives ranged from 0.03–0.88 and 0.1–1.0 μg/100 g among the three cereal samples. The absolute recoveries of most folate derivatives were 72–96 % for these cereal samples, with the exception of dihydrofolate, tetrahydrofolate, and folic acid (44–65 %). The inter-day and intra-day precisions were < 12 % for the three cereals. Analysis of folate content and composition in several cereal grains showed that the total folate levels were approximately 26−37 μg/100 g, with 5-methyl-tetrahydrofolate and 5-methyl-tetrahydrofolate as the dominant. MeFox, an oxidation product of 5-methyltetrahydrofolate, was detected at concentrations 20–39-fold higher than those of total folates in rice and wheat grains. This validated method is an efficient approach for folate determination in cereal crops.
Disciplines :
Chemistry
Author, co-author :
Shahid, Mehmood; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Lian, Tong ; Université de Liège - ULiège > TERRA Research Centre ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Wan, Xing; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Jiang, Ling; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Han, Lida; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Zhang, Chunyi; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Liang, Qiuju; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Folate monoglutamate in cereal grains: Evaluation of extraction techniques and determination by LC-MS/MS
National Special Program for Transgenic Research Technology Innovation Program of the Chinese Academy of Agricultural Sciences Shanghai Agriculture Applied Technology Development Program Collaborative Innovation Action, Agricultural Science
Funders :
NSCF - National Natural Science Foundation of China [CN] Ministry of Science and Technology of the People's Republic of China [CN]
Funding text :
This work was supported by the National Natural Science Foundation of China ( 31970326 ), National Special Program for Transgenic Research ( 2016ZX08003-002 ), Ministry of Science and Technology of China ( 2016YFD0100503 ), National Natural Science Foundation of China ( 31870283 ), Shanghai Agriculture Applied Technology Development Program ( Z20180103 ), and Collaborative Innovation Action, Agricultural Science , and Technology Innovation Program of the Chinese Academy of Agricultural Sciences ( CAAS-XTCX2016009 ).This work was supported by the National Natural Science Foundation of China (31970326), National Special Program for Transgenic Research (2016ZX08003-002), Ministry of Science and Technology of China (2016YFD0100503), National Natural Science Foundation of China (31870283), Shanghai Agriculture Applied Technology Development Program (Z20180103), and Collaborative Innovation Action, Agricultural Science, and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-XTCX2016009).
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