[en] As essential B vitamin for humans, folates accumulation in edible parts of crops, such as maize kernels, is of great importance for human health. But its breeding is always limited by the prohibitive cost of folate profiling. The molecular breeding is a more executable and efficient way for folate fortification, but is limited by the molecular knowledge of folate regulation. Here we report the genetic mapping of folate quantitative trait loci (QTLs) using a segregated population crossed by two maize lines, one high in folate (GEMS31) and the other low in folate (DAN3130). Two folate QTLs on chromosome 5 were obtained by the combination of F2 whole-exome sequencing and F3 kernel-folate profiling. These two QTLs had been confirmed by bulk segregant analysis using F6 pooled DNA and F7 kernel-folate profiling, and were overlapped with QTLs identified by another segregated population. These two QTLs contributed 41.6% of phenotypic variation of 5-formyltetrahydrofolate, the most abundant storage form among folate derivatives in dry maize grains, in the GEMS31×DAN3130 population. Their fine mapping and functional analysis will reveal details of folate metabolism, and provide a basis for marker-assisted breeding aimed at the enrichment of folates in maize kernels.
Disciplines :
Genetics & genetic processes
Author, co-author :
Guo, Wenzhu ✱; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Lian, Tong ✱; Université de Liège - ULiège > TERRA Research Centre ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Wang, Baobao ✱; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Guan, Jiantao ✱; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Yuan, Dong; Department of Chemistry and Chemical Engineering, Qilu Normal University, Jinan, 250200, China
Wang, Huan; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Safiul Azam, Fardous Mohammad; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Wan, Xing; School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China
Wang, Weixuan; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Liang, Qiuju; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Wang, Haiyang; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Tu, Jinxing; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Zhang, Chunyi; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Jiang, Ling; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
NSCF - National Natural Science Foundation of China
Funding text :
This work was financially supported by the Ministry of Science and Technology of China (2016YFD0100503 to L.J.), the National Natural Science Foundation of China (31870283 to L.J.), Shanghai Agriculture Applied Technology Development Program (Z20180103 to L.J.), Beijing Natural Science Foundation (6172032 to B.W.).
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