Comparative Transcriptome Analysis Reveals Mechanisms of Folate Accumulation in Maize Grains.

WGCNA; folate accumulation; maize kernel; transcriptome; Folic Acid; Edible Grain/metabolism; Folic Acid/metabolism; Gene Expression Profiling; Zea mays/genetics; Zea mays/metabolism; Transcriptome; Zea mays; Molecular Biology; Spectroscopy; Computer Science Applications

Abstract :

[en] Previously, the complexity of folate accumulation in the early stages of maize kernel development has been reported, but the mechanisms of folate accumulation are unclear. Two maize inbred lines, DAN3130 and JI63, with different patterns of folate accumulation and different total folate contents in mature kernels were used to investigate the transcriptional regulation of folate metabolism during late stages of kernel formation by comparative transcriptome analysis. The folate accumulation during DAP 24 to mature kernels could be controlled by circumjacent pathways of folate biosynthesis, such as pyruvate metabolism, glutamate metabolism, and serine/glycine metabolism. In addition, the folate variation between these two inbred lines was related to those genes among folate metabolism, such as genes in the pteridine branch, para-aminobenzoate branch, serine/tetrahydrofolate (THF)/5-methyltetrahydrofolate cycle, and the conversion of THF monoglutamate to THF polyglutamate. The findings provided insight into folate accumulation mechanisms during maize kernel formation to promote folate biofortification.

Disciplines :

Genetics & genetic processes
Biochemistry, biophysics & molecular biology

Author, co-author :

Lian, Tong ; Université de Liège - ULiège > TERRA Research Centre ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China

Wang, Xuxia; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China

Li, Sha ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Jiang, Haiyang; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China

Zhang, Chunyi; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China

Wang, Huan ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China ; National Agricultural Science and Technology Center, Chengdu 610213, China

Jiang, Ling ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Language :

English

Title :

Comparative Transcriptome Analysis Reveals Mechanisms of Folate Accumulation in Maize Grains.

Publication date :

01 February 2022

Journal title :

International Journal of Molecular Sciences

ISSN :

1661-6596

eISSN :

1422-0067

Publisher :

MDPI, Switzerland

Volume :

Issue :

Pages :

1708

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.mdpi.com/1422-0067/23/3/1708/pdf

Funding text :

This research was funded by the National Key Research and Development Program of China (2021YFF1000300 to C.Z.), The National Key Research and Development Program of China (2018YFD1000702/2018YFD1000700 to H.W.), The National Natural Science Foundation of China (31870283 to L.J.), and The Agricultural Science and Technology Innovation Program (ASTIP; CAAS-ZDRW202004 to C.Z. and H.W.). We thank the public laboratory of the Biotechnology Research Institute, Chinese Academy of Agricultural Sciences for providing us with access to the HPLC and triple-quad-rupole MS/MS instruments and for providing technical assistance. We would like to express our gratitude to Herv? Vanderchuren of the University of Li?ge for his thoughtful suggestions and com-ments on this study.

Available on ORBi :

since 25 May 2022

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