Article (Scientific journals)
Optimisation of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode
Hamilton, Christopher; Hiligsmann, Serge; Beckers, Laurent et al.
2010In International Journal of Hydrogen Energy, 35, p. 1089-1098
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Keywords :
Biohydrogen; Citrobacter freundii; pH; ammonia; iron; dilution
Abstract :
[en] Investigations were carried out to determine the effect of the pH, the nitrogen source, iron and the dilution rate (h 1) on fermentative hydrogen production from glucose by the newly isolated strain Citrobacter freundii CWBI952. The hydrogen production rate (HPR), hydrogen yield, biomass and soluble metabolites were monitored at 30 C in 100 mL serum bottles and in a 2.3 L bioreactor operated in batch, sequenced-batch and semicontinuous mode. The results indicate that hydrogen production activity, formate biosynthesis and glucose intake rates are very sensitive to the culture pH, and that additional formate bioconversion and production of hydrogen with lower biomass yields can be obtained at pH 5.9. In a further series of cultures casein peptone was replaced by (NH4)2SO4, a low cost alternative nitrogen source. The ammonia-based substitute was found to be suitable for H2 production when a concentration of 0.045 g/L FeSO4 was provided. Optimal overall performances (ca. an HPR of 33.2 mL H2/L h and a yield of 0:83 molH2 =molglucose) were obtained in the semicontinuous culture applying the previously optimized parameters for pH, nitrogen, and iron with a dilution rate of 0.012 h 1 and degassing of biogas by N2 at a 28 mL/min flow rate.
Research center :
Centre Wallon de Biologie Industrielle
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Biotechnology
Author, co-author :
Hamilton, Christopher  ;  Université de Liège - ULiège > Centre Wallon de biologie industrielle
Hiligsmann, Serge  ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Beckers, Laurent ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Masset, Julien ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Wilmotte, Annick  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
 These authors have contributed equally to this work.
Language :
English
Title :
Optimisation of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode
Publication date :
2010
Journal title :
International Journal of Hydrogen Energy
ISSN :
0360-3199
eISSN :
1879-3487
Publisher :
Pergamon Press - An Imprint of Elsevier Science
Volume :
35
Pages :
1089-1098
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Micro-H2 : Production microbiologique d’hydrogène : Etude des processus microalgal et bacterien
Funders :
Action de Recherches concertées ARC 07/12 04- ULg- Communauté française
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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