[en] Genetically engineered mouse models offer essential opportunities to investigate the mechanisms of initiation and progression in melanoma. Here, we report a new simplified histopathology classification of mouse melanocytic lesions in Tyr::NRASQ 61K derived models, using an interactive decision tree that produces homogeneous categories. Reproducibility for this classification system was evaluated on a panel of representative cases of murine melanocytic lesions by pathologists and basic scientists. Reproducibility, measured as inter-rater agreement between evaluators using a modified Fleiss’ kappa statistic, revealed a very good agreement between observers. Should this new simplified classification be adopted, it would create a robust system of communication between researchers in the field of mouse melanoma models.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sohier, Pierre; Institut Curie, INSERM U1021, Normal and Pathological Development of Melanocytes, PSL Research University, Orsay, France ; CNRS UMR3347, Univ Paris-Sud, Univ Paris-Saclay, Orsay, France ; Equipe Labellisée Ligue Contre le Cancer, Orsay, France
Legrand, Léa; INSERM U1053, Team 3 Oncogenesis of Cutaneous Lymphomas, Univ. Bordeaux, Bordeaux, France ; Pathology Department, CHU Bordeaux, Pessac, France
Aktary, Zackie; Institut Curie, INSERM U1021, Normal and Pathological Development of Melanocytes, PSL Research University, Orsay, France ; CNRS UMR3347, Univ Paris-Sud, Univ Paris-Saclay, Orsay, France ; Equipe Labellisée Ligue Contre le Cancer, Orsay, France
Grill, Christine ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale ; Institut Curie, INSERM U1021, Normal and Pathological Development of Melanocytes, PSL Research University, Orsay, France ; CNRS UMR3347, Univ Paris-Sud, Univ Paris-Saclay, Orsay, France ; Equipe Labellisée Ligue Contre le Cancer, Orsay, France
Delmas, Véronique; Institut Curie, INSERM U1021, Normal and Pathological Development of Melanocytes, PSL Research University, Orsay, France ; CNRS UMR3347, Univ Paris-Sud, Univ Paris-Saclay, Orsay, France ; Equipe Labellisée Ligue Contre le Cancer, Orsay, France
Bernex, Florence; IRCM, Inserm, ICM, Univ Montpellier, Montpellier, France
Reyes-Gomez, Edouard; INRA, UMR955 Génétique Fonctionnelle et Médicale, Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France ; UMR955 Génétique Fonctionnelle et Médicale, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France ; Unité d'Embryologie, d'Histologie et d'Anatomie Pathologique, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France
Larue, Lionel ; Institut Curie, INSERM U1021, Normal and Pathological Development of Melanocytes, PSL Research University, Orsay, France ; CNRS UMR3347, Univ Paris-Sud, Univ Paris-Saclay, Orsay, France ; Equipe Labellisée Ligue Contre le Cancer, Orsay, France
Vergier, Béatrice; INSERM U1053, Team 3 Oncogenesis of Cutaneous Lymphomas, Univ. Bordeaux, Bordeaux, France ; Pathology Department, CHU Bordeaux, Pessac, France
Language :
English
Title :
A histopathological classification system of Tyr::NRASQ61K murine melanocytic lesions: A reproducible simplified classification
INCa - Institut National du Cancer Ligue Nationale Contre le Cancer ITMO Cancer ANR - Agence Nationale de la Recherche
Funding text :
This work was supported by the Ligue Contre le Cancer—comité de l’Oise, PAIR melanoma, INCa, ITMO Cancer, and is under the program «Investissements d’Avenir» launched by the French Government and implemented by ANR Labex CelTisPhyBio (ANR-11-LBX-0038 and ANR-10-IDEX-0001-02 PSL). PS has a fellowship from INSERM, and ZA and CG from ARC. We are grateful to Maxime Battistella, Arnaud De LaThis work was supported by the Ligue Contre le Cancer?comit? de l'Oise, PAIR melanoma, INCa, ITMO Cancer, and is under the program ?Investissements d'Avenir? launched by the French Government and implemented by ANR Labex CelTisPhyBio (ANR-11-LBX-0038 and ANR-10-IDEX-0001-02 PSL). PS has a fellowship from INSERM, and?ZA and CG from ARC. We are grateful to Maxime Battistella, Arnaud De La Fourchardi?re, Giorgia Egidy, Laurent Le Cam, and Enrico Radaelli for helpful discussions.
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