[en] BACKGROUND: Propofol is a widely used intravenous general anesthetic. Acetylcholine (ACh) is critical in controlling epithelial ion transport. This study was to investigate the effects of propofol on ACh-evoked secretion in rat ileum epithelium.
METHODS: The Ussing chamber technique was used to investigate the effects of propofol on carbachol (CCh)-evoked short-circuit currents (Isc).
KEY RESULTS: Propofol (10-2 -10-6 mol/L) attenuated CCh-evoked Isc of rat ileum mucosa in a dose-dependent manner. The inhibitory effect of propofol was only evident after application to the serosal side. Pretreatment with tetrodotoxin (TTX, 0.3 μmol/L, n=5) had no effect on propofol-induced inhibitory effect, whereas serosal application of K+ channel inhibitor, glibenclamide, but not, an ATP-sensitive K+ channel inhibitor, largely reduced the inhibitory effect of propofol. In addition, pretreatment with either hexamethonium bromide (HB, nicotinic nACh receptor antagonist) or Cl- channel blockers niflumic acid and cystic fibrosis transmembrane conductance regulator (inh)-172 did not produce any effect on the propofol-induced inhibitory effect.
CONCLUSIONS & INFERENCES: Propofol inhibits CCh-induced intestinal secretion by directly targeting basolateral K+ channels.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Tang, S-H; Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, China
Wang, H-Y; Department of Physiology, School of Medicine, Shandong University, Jinan, China
Sun, H; Department of Thoracic Surgery, Shandong Tumor Hospital, Shandong University, Jinan, China
An, Ning ; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Cancer Signaling ; Department of Physiology, School of Medicine, Shandong University, Jinan, China
Xiao, L; Department of Physiology, School of Medicine, Shandong University, Jinan, China
Sun, Q; Department of Physiology, School of Medicine, Shandong University, Jinan, China
Zhao, D-B; Department of Thoracic Surgery, Shandong Tumor Hospital, Shandong University, Jinan, China
Language :
English
Title :
Propofol inhibits carbachol-induced chloride secretion by directly targeting the basolateral K+ channel in rat ileum epithelium.
This study was granted by the Fundamental Research Funds of Shandong University (2014QLKY08). This work was supported by the Fundamental Research Funds of Shandong University (2014QLKY08).
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