[en] Changes in extracellular osmolarity lead to alteration in cellular volume. In the study, we examined the effects of hyperosmolarity on short-circuit currents (Isc) in the rat ileum using the Ussing chamber technique. Mucosal exposure to 20 mM glucose evoked a decrease of ISC in the rat ileum, which was antagonized by the stretch-activated channel blocker GdCl3, TTX and atropine, respectively. In contrast, it was not blocked by phlorizin, a Na+-glucose cotransporter SGLT1 inhibitor. Furthermore, the unabsorbed substances, such as sucrose, lactulose or urea, also induced a decrease of ISC in rat ileum. ELISA results revealed that 20 mM glucose stimulated the release of histamine from rat ileum mucosa, which was attenuated by TTX. In addition, the glucose-induced ISC was depressed by pyrilamine, a histamine H1 receptor blocker (H1 antagonist) whereas it was not affected by ranitidine (H2 antagonist), clobenpropit (H3 antagonists) or JNJ7777120 (H4 antagonist), respectively. The ion substitution experiments suggest that the changes of Na+ and HCO3- ion flux underlie the glucose-induced ISC. In conclusion, osmotic stimulus decreased the basal ISC of rat ileum by evoking histamine release from ileum mucosa. The changes of Na+ and HCO3- ion transport are involved in the glucose-evoked decrease of basal ISC.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Wang, Banqin ✱; Department of Physiology, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, Jinan, China
An, Ning ✱; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Cancer Signaling ; Department of Physiology, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, Jinan, China, Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Sciences, Jinan, China
Shaikh, Abdul Sami; Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan, China
Wang, Haoyi; Department of Physiology, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, Jinan, China
Xiao, Ling; Department of Physiology, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, Jinan, China
Liu, Hongwei; Department of Physiology, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, Jinan, China
Li, Jingxin; Department of Physiology, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, Jinan, China
Zhao, Dongbo; Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Sciences, Jinan, China. Electronic address: 1172296684@qq.com
✱ These authors have contributed equally to this work.
Language :
English
Title :
Hyperosmolarity evokes histamine release from ileum mucosa by stimulating a cholinergic pathway.
Publication date :
2017
Journal title :
Biochemical and Biophysical Research Communications
NSCF - National Natural Science Foundation of China
Funding text :
This work was supported by the National Key Research and Development Program of China ( 2016YFC1302203 ) and the National Natural Science Foundation of China (NSFC 31571183 ).
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