Capillary electrophoresis (CE); Magnetic siRNA nanovectors (MSN); Purification; Small interfering RNA; Superparamagnetic iron oxide nanoparticles (SPION)
Abstract :
[en] Gene therapy and particularly small interfering RNA (siRNA) is a promising therapeutic method for treatment of various human diseases, especially cancer. However the lack of an ideal delivery system limits its clinical applications. Effective anticancer drug development represents the key for translation of research advances into medicines. Previously we reported, the optimization of magnetic siRNA nanovectors (MSN) formulation based on superparamagnetic iron oxide nanoparticles (SPION) and chitosan for systemic administration. This work aimed at using rational design to further optimize and develop MSN. Therefore, formulated MSN were first purified, then their physical and chemical properties were studied mainly through capillary electrophoresis. 95% of siRNA was found enclosed within the purified MSN (pMSN). pMSN showed colloidal stability at pH 7.4, effective protection of siRNA against ribonuclease degradation up to 24 hours and few siRNA release (less than 10%) at pH 7.4. These findings push toward further evaluation studies in vitro and/or in vivo, indicating the appropriateness of pMSN for cancer theranostics.
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
Abdelrahman, Mohammed; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France, Department of Pharmaceutics, Faculty of Pharmacy, University of Gezira, P.O. Box 20, Wad Medani, Sudan
Douziech Eyrolles, Laurence; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France. Electronic address: douziech.eyrolles@univ-tours.fr
Alkarib, Suad Y; Department of Pharmaceutics, College of Pharmacy, Karary University, Khartoum, Sudan
Hervé-Aubert, Katel; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France
Ben Djemaa, Sanaa ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques ; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France
Marchais, Hervé; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France
Chourpa, Igor; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France
David, Stephanie; Université François-Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, 37200 Tours, France
Language :
English
Title :
siRNA delivery system based on magnetic nanovectors: Characterization and stability evaluation.
INCa - Institut National du Cancer Fondation ARC pour la Recherche sur le Cancer LNCC - Ligue Nationale Contre le Cancer
Funding text :
The authors would like to thank the ?Institut National du Cancer (INCa)?, the ?Fondation ARC? and the ?Ligue nationale contre le cancer (LNCC)? (ARC_INCa_LNCC_7636) for the financial support and Didier Bedin (EA6295, ?Nanom?dicaments et Nanosondes?, University Fran?ois Rabelais of Tours) for technical support.
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