[en] Naranjilla (Solanum quitoense) is a perennial shrub plant mainly cultivated in Ecuador, Colombia, and Central America where it represents an important cash crop. Current cultivation practices not only cause deforestation and large-scale soil degradation but also make plants highly susceptible to pests and diseases. The use of arbuscular mycorrhizal fungi (AMF) can offer a possibility to overcome these problems. AMF can act beneficially in various ways, for example by improving plant nutrition and growth, water relations, soil structure and stability and protection against biotic and abiotic stresses. In this study, the impact of AMF inoculation on growth and nutrition parameters of naranjilla has been assessed. For inoculation three European reference AMF strains (Rhizoglomus irregulare, Claroideoglomus claroideum, and Cetraspora helvetica) and soils originating from three differently managed naranjilla plantations in Ecuador (conventional, organic, and permaculture) have been used. This allowed for a comparison of the performance of exotic AMF strains (reference strains) versus native consortia contained in the three soils used as inocula. To study fungal communities present in the three soils, trap cultures have been established using naranjilla as host plant. The community structures of AMF and other fungi inhabiting the roots of trap cultured naranjilla were assessed using next generation sequencing (NGS) methods. The growth response experiment has shown that two of the three reference AMF strains, a mixture of the three and soil from a permaculture site led to significantly better acquisition of phosphorus (up to 104%) compared to uninoculated controls. These results suggest that the use of AMF strains and local soils as inoculants represent a valid approach to improve nutrient uptake efficiency of naranjilla and consequently to reduce inputs of mineral fertilizers in the cultivation process. Improved phosphorus acquisition after inoculation with permaculture soil might have been caused by a higher abundance of AMF and the presence of Piriformospora indica as revealed by NGS. A higher frequency of AMF and enhanced root colonization rates in the trap cultures supplemented with permaculture soil highlight the importance of diverse agricultural systems for soil quality and crop production.
Symanczik, Sarah; Department of Soil Sciences, Research Institute of Organic AgricultureFrick, Switzerland
Gisler, Michelle; Department of Soil Sciences, Research Institute of Organic AgricultureFrick, Switzerland ; Department of Environmental Sciences, University of BaselBasel, Switzerland
Thonar, Cécile ; Université de Liège - ULiège > Département GxABT > Plant Sciences ; Department of Soil Sciences, Research Institute of Organic AgricultureFrick, Switzerland
Schlaeppi, Klaus; Department of Agroecology and Environment, AgroscopeZürich, Switzerland
Van der Heijden, Marcel; Department of Agroecology and Environment, AgroscopeZürich, Switzerland
Kahmen, Ansgar; Department of Environmental Sciences, University of BaselBasel, Switzerland
Boller, Thomas; Department of Environmental Sciences, University of BaselBasel, Switzerland
Mäder, Paul; Department of Soil Sciences, Research Institute of Organic AgricultureFrick, Switzerland
Language :
English
Title :
Application of Mycorrhiza and Soil from a Permaculture System Improved Phosphorus Acquisition in Naranjilla.
FP7 - 312117 - BIOFECTOR - Resource Preservation by Application of BIOefFECTORs in European Crop Production
Funders :
SNF - Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung SBFI - Staatssekretariat für Bildung, Forschung und Innovation EU - European Union
Funding text :
We thank Hannes Gamper from ETH Z?rich for providing the two inocula of Ce. helvetica and Cl. claroideum for our experiments. We acknowledge Alain Held (Agroscope) and Giancarlo Russo and Andrea Patrignani (Functional Genomic Centre Zurich) for excellent support in library preparation and sequencing. We also thank Pepe Proa?io and David Sanchez (Ministerio de Agricultura Ganader?a Acuacultura y Pesca) for providing us with naranjilla seeds and soil samples, respectively, as well as Jesus und Victor Tepud from La Esperanza and naranjilla farmers from Carchi for sharing seeds, soils samples and valuable information.Projects of KS are supported by the Swiss State Secretariat for Education, Research and Innovation (Projects C14.0132, C15.0111), and the Swiss National Science Foundation (Nr. 31003A_165891). SS partially received funding from the European Community?s Seventh Framework Program 662 (FP7/2007?2013) under Grant Agreement No. 312117 (BIOFECTOR), the European Union?s Horizon 2020 research and innovation programme under grant agreement No. 635750 and from the Swiss State Secretary for Education, Research and Innovation (SERI) under contact number 635750.
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