Reference : Lysozyme gene activity in chicken macrophages is controlled by positive and negative ...
Scientific journals : Article
Life sciences : Genetics & genetic processes
Lysozyme gene activity in chicken macrophages is controlled by positive and negative regulatory elements.
Steiner, Christoph [> > > >]
Muller, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Baniahmad, Aria [> > > >]
Renkawitz, Rainer [> > > >]
Nucleic Acids Research
Oxford University Press
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Base Sequence ; Cell Line ; Chickens/metabolism ; Enhancer Elements, Genetic ; Fibroblasts/metabolism ; Gene Expression Regulation ; Genes ; Genetic Vectors ; Hematopoietic Stem Cells/metabolism ; Macrophages/metabolism ; Muramidase/biosynthesis/genetics ; Organ Specificity ; Recombinant Fusion Proteins/biosynthesis/genetics
[en] The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct. To investigate which DNA elements are involved in the control of its expression in macrophages we performed transient DNA transfer experiments with two different types of plasmids: 5'-deletion mutants of the upstream region of the chicken lysozyme gene and different fragments from this area in front of the thymidine kinase promoter (herpes simplex virus), each placed in front of the CAT (chloramphenicol acetyl transferase) coding sequence. Two enhancers (E-2.7 kb and E-0.2 kb) were characterized. They are active in macrophages, but not in chicken fibroblasts. Furthermore a negative element (N-2.4 kb) was identified, which is active in fibroblasts and promyelocytes, but not in mature macrophages. The combined action of all three elements contributes to the observed lysozyme gene activities: no activity in fibroblasts, moderate activity in promyelocytes and high activity in mature macrophages.

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