SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data. - 2021
SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data.
[en] We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the reference reagent and standard curve obtained by testing a dilution series reduced the inter-laboratory variability ca 10-fold. The harmonisation of neutralising antibody quantification is a vital step towards determining the protective and therapeutic levels of neutralising antibodies.
Disciplines :
Immunology & infectious disease
Author, co-author :
Nguyen, Dung; University of Oxford, Oxford, United Kingdom.
Simmonds, Peter; University of Oxford, Oxford, United Kingdom.
Steenhuis, Maurice; Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic
Wouters, Elise; Transfusion Research Centre, Belgian Red Cross-Flanders, Ghent, Belgium.
Desmecht, Daniel ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Garigliany, Mutien-Marie ; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire ; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie générale et autopsies
Barbezange, Cyril; National Influenza Centre, Sciensano, Brussels, Belgium.
Maes, Piet; KU Leuven, Rega Institute, Clinical and Epidemiological Virology, Leuven, Belgium.
Van Holm, Bram; KU Leuven, Rega Institute, Clinical and Epidemiological Virology, Leuven, Belgium.
Mendoza, Joaquín; Vircell SL, Granada, Spain.
Oyonarte, Salvador; Andalusian Network of Transfusion Medicine, Tissues and Cells, Sevilla, Spain.
Fomsgaard, Anders; Virus and Microbiological Special Diagnostics, Statens Serum Institute, Copenhagen,
Lassaunière, Ria; Virus and Microbiological Special Diagnostics, Statens Serum Institute, Copenhagen,
Zusinaite, Eva; Tartu University Institute of Technology, Tartu, Estonia.
Resman Rus, Katarina; Institute of Microbiology and Immunology, Ljubljana, Slovenia.
Avšič-Županc, Tatjana; Institute of Microbiology and Immunology, Ljubljana, Slovenia.
Reimerink, Johan Hj; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML,
Brouwer, Fiona; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML,
Hoogerwerf, Marieke; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML,
Reusken, Chantal Bem; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML,
Grodeland, Gunnveig; Dep. of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
Le Cam, Sophie; Etablissement Français du Sang, La Plaine Saint Denis, France.
Gallian, Pierre; Etablissement Français du Sang, La Plaine Saint Denis, France. ; Unité des Virus Émergents (Aix-Marseille University - IRD 190 - Inserm 1207 - IHU
Amroun, Abdennour; Unité des Virus Émergents (Aix-Marseille University - IRD 190 - Inserm 1207 - IHU
Brisbarre, Nadège; Unité des Virus Émergents (Aix-Marseille University - IRD 190 - Inserm 1207 - IHU ; Etablissement français du Sang Provence Alpes Côte d'Azur et Corse, Marseille,
Martinaud, Christophe; Centre de Transfusion Sanguine des Armées, Clamart, France.
Leparc Goffart, Isabelle; Unité de Virologie, Institut de Recherche Biomédicale des Armées, Marseille, France.
Schrezenmeier, Hubert; Department of Transfusion Medicine, Ulm University, Ulm, Germany. ; Institute for Clinical Transfusion Medicine and Immunogenetics, German Red Cross
Feys, Hendrik B; Transfusion Research Centre, Belgian Red Cross-Flanders, Ghent, Belgium.
van der Schoot, C Ellen; Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic
Harvala, Heli; Microbiology Services, NHS Blood and Transplant, Colindale, United Kingdom. ; University College of London, London, United Kingdom.
SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data.
Publication date :
July 2021
Journal title :
Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles
ISSN :
1025-496X
eISSN :
1560-7917
Publisher :
Centre Europeen pour la Surveillance Épidemiologique du SIDA, Fr
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