[en] Vertical farming is considered as a potential solution to increase yield while decreasing resource use and pesticide impacts compared to conventional agriculture. However, the profitability of cultivating ordinary leafy green crops with low market prices in vertical farming is debated. We studied the agronomic feasibility and viability of growing a medicinal plant—Euphorbia peplus—for its ingenol-mebutate content in a modified shipping container farm as an alternative crop cultivation system. The impacts of three hydroponic substrates, three light intensities, three plant localizations and two surface areas on E. peplus yield and cost were tested in several scenarios. The optimization of biomass yield and area surface decreased the cultivation cost, with fresh crop cost per kg ranging from €185 to €59. Three ingenol-mebutate extraction methods were tested. The best extraction yields and cheapest method can both be attributed to ethyl acetate at 120 °C, with a yield of 43.8 mg/kg at a cost of €38 per mg. Modeling of the profitability of a pharmaceutical gel based on ingenol-mebutate showed that economic feasibility was difficult to reach, but some factors could rapidly increase the profitability of this production.
Disciplines :
Agriculture & agronomy
Author, co-author :
Bafort, Françoise ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
Food and Agriculture Organization of the United Nations (FAO). Feeding the Cities of the Future. Available online: https://www.fao.org/news/story/en/item/446763/icode/(accessed on 31 January 2022).
Lotze-Campen, H.; Müller, C.; Bondeau, A.; Rost, S.; Popp, A.; Lucht, W. Global food demand, productivity growth, and the scarcity of land and water resources: A spatially explicit mathematical programming approach. Agric. Econ. 2008, 39, 325–338. [CrossRef]
Davis, K. The origin and growth of urbanization in the world. Am. J. Sociol. 1955, 60, 429–437. [CrossRef]
Agrilyst State of Indoor Farming. Available online: https://artemisag.com/wp-content/uploads/06/stateofindoorfarming-report-2017.pdf (accessed on 7 June 2021).
Kozai, T.; Niu, G. Chapter 1—Introduction. In Plant Factory, 2nd ed.; Kozai, T., Niu, G., Takagaki, M., Eds.; Academic Press: Cambridge, MA, USA, 2020; pp. 3–6. ISBN 978-0-12-816691-8.
Despommier, D. The rise of vertical farms. Sci. Am. 2009, 301, 80–87. [CrossRef] [PubMed]
Avgoustaki, D.D.; Xydis, G. How energy innovation in indoor vertical farming can improve food security, sustainability, and food safety? Adv. Food Secur. Sustain. 2020, 5, 1–51. [CrossRef]
Avgoustaki, D.D.; Xydis, G. Indoor vertical farming in the urban nexus context: Business growth and resource savings. Sustainability 2020, 12, 1965. [CrossRef]
Barbosa, G.L.; Gadelha, F.D.A.; Kublik, N.; Proctor, A.; Reichelm, L.; Weissinger, E.; Wohlleb, G.M.; Halden, R.U. Comparison of land, water, and energy requirements of lettuce grown using hydroponic vs. conventional agricultural methods. Int. J. Environ. Res. Public Health 2015, 12, 6879–6891. [CrossRef]
Banerjee, C.; Adenaeuer, L. Up, up and away! The economics of vertical farming. J. Agric. Stud. 2014, 2, 40–60. [CrossRef]
Benke, K.; Tomkins, B. Future food-production systems: Vertical farming and controlled-environment agriculture. Sustain. Sci. Pract. Policy 2017, 13, 13–26. [CrossRef]
Beacham, A.M.; Vickers, L.H.; Monaghan, J.M. Vertical farming: A summary of approaches to growing skywards. J. Hortic. Sci. Biotechnol. 2019, 94, 277–283. [CrossRef]
Coyle, B.D.; Ellison, B. Will consumers find vertically farmed produce “out of reach”? Choices 2017, 32, 1–8.
Cox, S.; Van Tassel, D. Vertical farming doesn’t stack up. Synth. Regen. 2010, 52.
Benis, K.; Ferrao, P. Commercial farming within the urban built environment—Taking stock of an evolving field in northern countries. Glob. Food Secur. 2018, 17, 30–37. [CrossRef]
Graamans, L.; Baeza, E.; van den Dobbelsteen, A.; Tsafaras, I.; Stanghellini, C. Plant factories versus greenhouses: Comparison of resource use efficiency. Agric. Syst. 2018, 160, 31–43. [CrossRef]
Sparks, R.E.; Stwalley, R.M., III. Design and testing of a modified hydroponic shipping container system for urban food production. Int. J. Appl. Agric. Sci. 2018, 4, 93–102.
Debusschere, T.; Boekhout, R. When Will Vertical Farming Become Profitable? Available online: https://www.verticalfarmdaily. com/article/9321424/when-will-vertical-farming-become-profitable/(accessed on 6 August 2021).
VerticalFarmDaily Not Possible to Market Our Vertically Grown Vegetables in a Financially Attractive Way. Available online: https://www.verticalfarmdaily.com/article/9349889/not-possible-to-market-our-vertically-grown-vegetables-in-a-financially-attractive-way/(accessed on 31 August 2021).
Eaves, J.; Eaves, S. Comparing the profitability of a greenhouse to a vertical farm in Quebec. Can. J. Agric. Econ. Can.. 2018, 66, 43–54. [CrossRef]
Li, L.; Li, X.; Chong, C.; Wang, C.-H.; Wang, X. A decision support framework for the design and operation of sustainable urban farming systems. J. Clean. Prod. 2020, 268, 121928. [CrossRef]
Howitz, K.T.; Sinclair, D.A. Xenohormesis: Sensing the chemical cues of other species. Cell 2008, 133, 387–391. [CrossRef] [PubMed]
Newman, D.J.; Cragg, G.M. Natural products as sources of new drugs over the last 25 years. J. Nat. Prod. 2007, 70, 461–477. [CrossRef] [PubMed]
World Health Organization. Traditional medicine: Report by the secretariat. In Proceedings of the 56th World Health Assembly, Geneva, Switzerland, 19–28 May 2003.
Ernst, M.; Grace, O.M.; Saslis-Lagoudakis, H.; Nilsson, N.; Simonsen, H.T.; Rønsted, N. Global medicinal uses of Euphorbia L. (Euphorbiaceae). J. Ethnopharmacol. 2015, 176, 90–101. [CrossRef] [PubMed]
Frezza, C.; Venditti, A.; Sciubba, F.; Tomai, P.; Antonetti, M.; Franceschin, M.; Di Cocco, M.E.; Gentili, A.; Delfini, M.; Serafini, M.; et al. Phytochemical profile of Euphorbia peplus L. collected in Central Italy and NMR semi-quantitative analysis of the diterpenoid fraction. J. Pharm. Biomed. Anal. 2018, 160, 152–159. [CrossRef] [PubMed]
Batanouny, K.H.; Stichler, W.; Ziegler, H. Photosynthetic pathways and ecological distribution of Euphorbia species in Egypt. Oecologia 1991, 87, 565–569. [CrossRef] [PubMed]
Berman, B. New developments in the treatment of actinic keratosis: Focus on ingenol mebutate gel. Clin. Cosmet. Investig. Dermatol. 2012, 5, 111–122. [CrossRef] [PubMed]
Verma, N.; Shukla, S. Impact of various factors responsible for fluctuation in plant secondary metabolites. J. Appl. Res. Med. Aromat. Plants 2015, 2, 105–113. [CrossRef]
Akula, R.; Ravishankar, G.A. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal. Behav. 2011, 6, 1720–1731. [CrossRef] [PubMed]
Dou, H.; Niu, G. Chapter 9—Plant responses to light. In Plant Factory, 2nd ed.; Kozai, T., Niu, G., Takagaki, M., Eds.; Academic Press: Cambridge, MA, USA, 2020; pp. 153–166. ISBN 978-0-12-816691-8.
Dou, H.; Niu, G.; Gu, M.; Masabni, J. Morphological and physiological responses in basil and brassica species to different proportions of red, blue, and green wavelengths in indoor vertical farming. J. Am. Soc. Hortic. Sci. 2020, 145, 267–278. [CrossRef]
Raviv, M.; Wallach, R.; Silber, A.; Bar-Tal, A. Substrates and their analysis. In Hydroponic Production of Vegetables and Ornamentals; Sawas, D., Passam, H., Eds.; Embrio Publications: Athens, Greece, 2002; pp. 25–105.
Deepagoda, T.C.; Lopez, J.C.C.; Møldrup, P.; De Jonge, L.W.; Tuller, M. Integral parameters for characterizing water, energy, and aeration properties of soilless plant growth media. J. Hydrol. 2013, 502, 120–127. [CrossRef]
Sonneveld, C.; Voogt, W. Plant nutrition in future greenhouse production. In Plant Nutrition of Greenhouse Crops; Springer: Dordrecht, The Netherlands, 2009; pp. 393–403. ISBN 978-90-481-2532-6.
Othman, Y.; Bataineh, K.; Al-Ajlouni, M.; Alsmairat, N.; Ayad, J.; Shiyab, S.; Al-Qarallah, B.; St Hilaire, R. Soilless culture: Management of growing substrate, water, nutrient, salinity, microorganism and product quality. Fresenius Environ. Bull. 2019, 28, 3249–3260.
Alsmairat, N.G.; Al-Ajlouni, M.G.; Ayad, J.Y.; Othman, Y.A.; Hilaire, R.S. Composition of soilless substrates affect the physiology and fruit quality of two strawberry (Fragaria × ananassa Duch.) cultivars. J. Plant Nutr. 2018, 41, 2356–2364. [CrossRef]
Al-Ajmi, A.; Al-Karaki, G.; Othman, Y. Effect of different substrates on fruit yield and quality of cherry tomato grown in a closed soilless system. In Proceedings of the Acta Horticulturae; International Society for Horticultural Science (ISHS): Leuven, Belgium, 2009; pp. 491–494.
Maloupa, E.; Gerasopoulos, D. Quality production of four cut gerberas in a hydroponic system of four substrates. In Proceedings of the Acta Horticulturae; International Society for Horticultural Science (ISHS): Leuven, Belgium, 1999; pp. 433–438.
Béres, T.; Dragull, K.; Pospíšil, J.; Tarkowská, D.; Dančák, M.; Bíba, O.; Tarkowski, P.; Doležal, K.; Strnad, M. Quantitative analysis of ingenol in Euphorbia species via validated isotope dilution ultra-high performance liquid chromatography tandem mass spectrometry. Phytochem. Anal. 2018, 29, 23–29. [CrossRef] [PubMed]
Tobiszewski, M.; Namieśnik, J.; Pena-Pereira, F. Environmental risk-based ranking of solvents using the combination of a multimedia model and multi-criteria decision analysis. Green Chem. 2017, 19, 1034–1042. [CrossRef]
Nothias-Scaglia, L.-F.; Dumontet, V.; Neyts, J.; Roussi, F.; Costa, J.; Leyssen, P.; Litaudon, M.; Paolini, J. LC-MS2-Based dereplication of Euphorbia extracts with anti-Chikungunya virus activity. Fitoterapia 2015, 105, 202–209. [CrossRef] [PubMed]
Cheng, J.; Han, W.; Wang, Z.; Shao, Y.; Wang, Y.; Zhang, Y.; Li, Z.; Xu, X.; Zhang, Y. Hepatocellular carcinoma growth is inhibited by Euphorbia helioscopia L. extract in nude mice xenografts. BioMed Res. Int. 2015, 2015, 601015. [CrossRef] [PubMed]
Araújo, J.M.; Sandi, D. Extraction of coffee diterpenes and coffee oil using supercritical carbon dioxide. Food Chem. 2007, 101, 1087–1094. [CrossRef]
Bar-Tal, A.; Saha, U.K.; Raviv, M.; Tuller, M. Chapter 7—Inorganic and synthetic organic components of soilless culture and potting mixtures. In Soilless Culture, 2nd ed.; Raviv, M., Lieth, J.H., Bar-Tal, A., Eds.; Elsevier: Boston, MA, USA, 2019; pp. 259–301. ISBN 978-0-444-63696-6.
De Swaef, T.; Verbist, K.; Cornelis, W.; Steppe, K. Tomato sap flow, stem and fruit growth in relation to water availability in rockwool growing medium. Plant Soil 2012, 350, 237–252. [CrossRef]
Deepagoda, T.C.; Moldrup, P.; Tuller, M.; Pedersen, M.; Lopez, J.C.C.; de Jonge, L.W.; Kawamoto, K.; Komatsu, T. Gas diffusivity-based design and characterization of greenhouse growth substrates. Vadose Zone J. 2013, 12, 1–13. [CrossRef]
Raatz, L.; Bacchi, N.; Pirhofer Walzl, K.; Glemnitz, M.; Müller, M.E.H.; Joshi, J.; Scherber, C. How much do we really lose?—Yield losses in the proximity of natural landscape elements in agricultural landscapes. Ecol. Evol. 2019, 9, 7838–7848. [CrossRef] [PubMed]
Schmidt, M.; Nendel, C.; Funk, R.; Mitchell, M.G.E.; Lischeid, G. Modeling yields response to shading in the field-to-forest transition zones in heterogeneous landscapes. Agriculture 2019, 9, 6. [CrossRef]
Board, J. Light interception efficiency and light quality affect yield compensation of soybean at low plant populations. Crop Sci. 2000, 40, 1285–1294. [CrossRef]
Kasperbauer, M.J. Far-red light reflection from green leaves and effects on phytochrome-mediated assimilate partitioning under field conditions. Plant Physiol. 1987, 85, 350–354. [CrossRef] [PubMed]
Dou, H.; Niu, G.; Gu, M.; Masabni, J.G. Responses of sweet basil to different daily light integrals in photosynthesis, morphology, yield, and nutritional quality. HortScience horts 2018, 53, 496–503. [CrossRef]
Wang, Y.; Folta, K.M. Contributions of green light to plant growth and development. Am. J. Bot. 2013, 100, 70–78. [CrossRef] [PubMed]
Terashima, I.; Fujita, T.; Inoue, T.; Chow, W.S.; Oguchi, R. Green light drives leaf photosynthesis more efficiently than red light in strong white light: Revisiting the enigmatic question of why leaves are green. Plant Cell Physiol. 2009, 50, 684–697. [CrossRef] [PubMed]
Dou, H.; Niu, G.; Gu, M. Photosynthesis, morphology, yield, and phytochemical accumulation in basil plants influenced by substituting green light for partial red and/or blue light. HortScience 2019, 54, 1769–1776. [CrossRef]
Hanson, D.T.; Sharkey, T.D. Effect of growth conditions on isoprene emission and other thermotolerance-enhancing compounds. Plant Cell Environ. 2001, 24, 929–936. [CrossRef]
Rosenfeld, H.J.; Aaby, K.; Lea, P. Influence of temperature and plant density on sensory quality and volatile terpenoids of carrot (Daucus carota L.) root. J. Sci. Food Agric. 2002, 82, 1384–1390. [CrossRef]
Helmig, D.; Ortega, J.; Duhl, T.; Tanner, D.; Guenther, A.; Harley, P.; Wiedinmyer, C.; Milford, J.; Sakulyanontvittaya, T. Sesquiterpene emissions from pine trees—Identifications, emission rates and flux estimates for the contiguous United States. Environ. Sci. Technol. 2007, 41, 1545–1553. [CrossRef] [PubMed]
Ibrahim, M.A.; Mäenpää, M.; Hassinen, V.; Kontunen-Soppela, S.; Malec, L.; Rousi, M.; Pietikäinen, L.; Tervahauta, A.; Kärenlampi, S.; Holopainen, J.; et al. Elevation of night-time temperature increases terpenoid emissions from Betula pendula and Populus tremula. J. Exp. Bot. 2010, 61, 1583–1595. [CrossRef] [PubMed]
Filella, I.; Wilkinson, M.J.; Llusià, J.; Hewitt, C.N.; Peñuelas, J. Volatile organic compounds emissions in Norway spruce (Picea abies) in response to temperature changes. Physiol. Plant. 2007, 130, 58–66. [CrossRef]
Kivimäenpää, M.; Riikonen, J.; Ahonen, V.; Tervahauta, A.; Holopainen, T. Sensitivity of Norway spruce physiology and terpenoid emission dynamics to elevated ozone and elevated temperature under open-field exposure. Environ. Exp. Bot. 2013, 90, 32–42. [CrossRef]
Petridis, A.; Therios, I.; Samouris, G.; Tananaki, C. Salinity-induced changes in phenolic compounds in leaves and roots of four olive cultivars (Olea europaea L.) and their relationship to antioxidant activity. Environ. Exp. Bot. 2012, 79, 37–43. [CrossRef]
Tounekti, T.; Vadel, A.M.; Ennajeh, M.; Khemira, H.; Munné-Bosch, S. Ionic interactions and salinity affect monoterpene and phenolic diterpene composition in rosemary (Rosmarinus officinalis). J. Plant Nutr. Soil Sci. 2011, 174, 504–514. [CrossRef]
Niessen, W.; Chanteux, A. Les Tableaux de Bord et Business Plan; Editions des Chambres de Commerce et d’Industrie de Wallonie, Ed.; Edipro: Seraing, Belgium, 2005.
Hohmann, J.; Evanics, F.; Berta, L.; Bartók, T. Diterpenoids from Euphorbia peplus. Planta Med. 2000, 66, 291–294. [CrossRef] [PubMed]
Liang, X.; Grue-Sørensen, G.; Petersen, A.K.; Högberg, T. Semisynthesis of ingenol 3-angelate (PEP005): Efficient stereoconserva-tive angeloylation of alcohols. Synlett 2012, 23, 2647–2652. [CrossRef]
Appendino, G.; Tron, G.C.; Cravotto, G.; Palmisano, G.; Annunziata, R.; Baj, G.; Surico, N. Synthesis of modified ingenol esters. Eur. J. Org. Chem. 1999, 1999, 3413–3420. [CrossRef]
Winkler, J.D.; Rouse, M.B.; Greaney, M.F.; Harrison, S.J.; Jeon, Y.T. The first total synthesis of (±)-ingenol. J. Am. Chem. Soc. 2002, 124, 9726–9728. [CrossRef] [PubMed]
Tanino, K.; Onuki, K.; Asano, K.; Miyashita, M.; Nakamura, T.; Takahashi, A.Y.; Kuwajima, I. Total synthesis of ingenol. J. Am. Chem. Soc. 2003, 125, 1498–1500. [CrossRef] [PubMed]
Nickel, A.; Maruyama, T.; Tang, H.; Murphy, P.D.; Greene, B.; Yusuff, A.N.; Wood, J.L. Total synthesis of ingenol. J. Am. Chem. Soc. 2004, 126, 16300–16301. [CrossRef] [PubMed]
McKerrall, S.J.; Jørgensen, L.; Kuttruff, C.A.; Ungeheuer, F.; Baran, P.S. Development of a concise synthesis of (+)-ingenol. J. Am. Chem. Soc. 2014, 136, 5799–5810. [CrossRef] [PubMed]
Morgan, S.; Grootendorst, P.; Lexchin, J.; Cunningham, C.; Greyson, D. The cost of drug development: A systematic review. Health Policy 2011, 100, 4–17. [CrossRef] [PubMed]
DiMasi, J.A.; Grabowski, H.G.; Hansen, R.W. Innovation in the pharmaceutical industry: New estimates of R&D costs. J. Health Econ. 2016, 47, 20–33. [CrossRef] [PubMed]
Le Galès, C. Pourquoi les nouveaux médicaments sont-ils si chers? Med. Sci. 2018, 34, 354–361. [CrossRef] [PubMed]
