[en] Lipopeptides produced by Bacillus subtilis display many activities (surfactant, antimicrobial, and antitumoral), which make them interesting compounds with a wide range of applications. During the past years, several processes have been developed to enable their production and purification with suitable yield and purity. The already implemented processes mainly end with a critical drying step, which is currently achieved by freeze-drying. In this study, the possibility to replace this freeze-drying step with a spray-drying one, more suited to industrial applications, was analyzed. After evaluating their thermal resistance, we have developed a spray-drying methodology applicable for the three lipopeptides families produced by B. subtilis, i.e., surfactin, mycosubtilin (iturin family), and plipastatin (fengycin family). For each lipopeptide, the spray-drying procedure was applied at three steps of the purification process by ultrafiltration (supernatant, diafiltered solution, and pre-purified fraction). The analysis of the activities of each spray-dried lipopeptide showed that this drying method is not decreasing its antimicrobial and biosurfactant properties. The methodology developed in this study enabled for the first time the spray-drying of surfactin, without adjuvants' addition and regardless of the purification step considered. In the case of fengycin and mycosubtilin, only diafiltered solution and purified fraction could be successfully spray-dried without the addition of adjuvant. Maltodextrin addition was also investigated as the solution for the direct drying of supernatant. As expected, the performances of the spray-drying step and the purity of the powder obtained are highly related to the purification step at which the product was dried. Interestingly, the impact of mycosubtilin concentration on spray-drying yield was also evidenced.
Disciplines :
Biotechnology
Author, co-author :
Vassaux, Antoine ; Université de Liège - ULiège ; Université de Lille, UMRt BioEcoAgro 1158-INRAE, Équipe Métabolites Secondaires d'Origine Microbienne, Institut Charles Viollette, Lille, France
Rannou, Marie; Université de Lille, UMRt BioEcoAgro 1158-INRAE, Équipe Métabolites Secondaires d'Origine Microbienne, Institut Charles Viollette, Lille, France
Peers, Soline; LIPOFABRIK, Villeneuve d'Ascq, France
Daboudet, Théo; Université de Lille, UMRt BioEcoAgro 1158-INRAE, Équipe Métabolites Secondaires d'Origine Microbienne, Institut Charles Viollette, Lille, France
Jacques, Philippe ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies ; LIPOFABRIK, Villeneuve d'Ascq, France
Coutte, François; Université de Lille, UMRt BioEcoAgro 1158-INRAE, Équipe Métabolites Secondaires d'Origine Microbienne, Institut Charles Viollette, Lille, France ; LIPOFABRIK, Villeneuve d'Ascq, France
Language :
English
Title :
Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis.
Interreg Bio-Based Industries Joint Undertaking FP7 Research for the Benefit of SMEs
Funding text :
This work was supported by the project ?BioSMART?Bio-based smart packaging for enhanced preservation of food quality??Grant agreement No. 745762, funded by the Bio-based Industries Joint Undertaking (BBI-JU) under the European Union?s Horizon 2020 Research and Innovation Programme. This work was also supported by the Universities of Lille and Li?ge from the European INTERREG V SmartBioControl/BioProd project and the Charles Viollette Institute by the ALIBIOTECH program funding administered by the Hauts-de-France Region and for Lipofabrik by the SME Instrument Grant agreement No. 849713 under the European Union?s Horizon 2020 Research and Innovation Programme. Met steun van het europees fonds voor regionale ontwikkeling. Avec le soutien du fonds europ?en de d?veloppement r?gional.This work was supported by the project “BioSMART—Bio-based smart packaging for enhanced preservation of food quality”—Grant agreement No. 745762, funded by the Bio-based Industries Joint Undertaking (BBI-JU) under the European Union’s Horizon 2020 Research and Innovation Programme. This work was also supported by the Universities of Lille and Liège from the European INTERREG V SmartBioControl/BioProd project and the Charles Viollette Institute by the ALIBIOTECH program funding administered by the Hauts-de-France Region and for Lipofabrik by the SME Instrument Grant agreement No. 849713 under the European Union’s Horizon 2020 Research and Innovation Programme. Met steun van het europees fonds voor regionale ontwikkeling. Avec le soutien du fonds européen de développement régional.
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