Evaluation of an alternative heterotopic transplantation model for ovarian tissue to test pharmaceuticals improvements for fertility restoration.

Terren, Carmen; Bindels, Jules; NISOLLE, Michelle; Noël, Agnès; Munaut, Carine

doi:10.1186/s12958-022-00910-9

Article (Scientific journals)

Evaluation of an alternative heterotopic transplantation model for ovarian tissue to test pharmaceuticals improvements for fertility restoration.

Terren, Carmen; Bindels, Jules; NISOLLE, Michelle et al.

2022 • In Reproductive Biology and Endocrinology, 20 (1), p. 35

Peer reviewed

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https://hdl.handle.net/2268/288702

DOI
10.1186/s12958-022-00910-9

PubMed
35183206

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Keywords :

Animal model; Fertility restoration; Heterotopic transplantation; Ovarian tissue transplantation; Developmental Biology; Endocrinology; Reproductive Medicine; Obstetrics and Gynecology

Abstract :

[en] BACKGROUND: Ovarian tissue cryopreservation and transplantation (OTCTP) is currently the main option available to preserve fertility in prepubertal patients undergoing aggressive cancer therapy treatments. However, a major limitation of OTCTP is follicle loss after transplantation. The mouse is a model of choice for studying ovarian function and follicle development after ovarian tissue grafting in vivo. In these mouse models, ovarian tissue or ovaries can be transplanted to different sites. Our aim was to evaluate a new alternative to heterotopic transplantation models that could be useful to test pharmaceutical improvement for ovarian grafts after OTCTP. METHODS: Slow frozen murine whole ovaries were transplanted into the mouse ears (between the external ear skin layer and the cartilage). Ovarian transplants were recovered after 3, 14 or 21 days. Grafts were analyzed by immunohistochemistry and follicle density analyses were performed. RESULTS: An increase of ovarian vascularization (CD31 and Dextran-FITC positive staining), as well as cellular proliferation (Ki67 staining) were observed 3 weeks after transplantation in comparison to 3 days. Fibrosis density, evaluated after Van Gieson staining, decreased 3 weeks after transplantation. Furthermore, transplantation of cryopreserved ovaries into ovariectomized mice favored follicle activation compared to transplantation into non-ovariectomized mice. CONCLUSION: The present study indicates that surgical tissue insertion in the highly vascularized murine ear is an effective model for ovarian grafting. This model could be helpful in research to test pharmaceutical strategies to improve the function and survival of cryopreserved and transplanted ovarian tissue.

Disciplines :

Reproductive medicine (gynecology, andrology, obstetrics)

Author, co-author :

Terren, Carmen ; Université de Liège - ULiège > GIGA > GIGA Cancer - Tumours and development biology

Bindels, Jules ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

NISOLLE, Michelle ; Université de Liège - ULiège > GIGA > GIGA Cancer

Noël, Agnès ; Université de Liège - ULiège > GIGA > GIGA Cancer

Munaut, Carine ; Université de Liège - ULiège > GIGA > GIGA Cancer - Tumours and development biology

Language :

English

Title :

Evaluation of an alternative heterotopic transplantation model for ovarian tissue to test pharmaceuticals improvements for fertility restoration.

Publication date :

19 February 2022

Journal title :

Reproductive Biology and Endocrinology

eISSN :

1477-7827

Publisher :

Springer Science and Business Media LLC, England

Volume :

Issue :

Pages :

Peer reviewed :

Peer reviewed

Additional URL :

https://link.springer.com/content/pdf/10.1186/s12958-022-00910-9.pdf

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
ULiège FSR - Université de Liège. Fonds spéciaux pour la recherche
Fondation Léon Fredericq
Belgian Foundation Against Cancer

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