Bacillus subtilis; Biocontrol; Lipopeptides; Mycosubtilin; Wheat; Zymoseptoria tritici; Biological Control Agents; Peptides, Cyclic; Ascomycota/drug effects; Ascomycota/growth & development; Bacillus subtilis/metabolism; Biological Control Agents/pharmacology; Lipopeptides/pharmacology; Peptides, Cyclic/pharmacology; Plant Diseases/prevention & control; Triticum/microbiology; Ascomycota; Plant Diseases; Triticum; Environmental Chemistry; Pollution; Health, Toxicology and Mutagenesis; General Medicine
Abstract :
[en] Innovation toward ecofriendly plant protection products compatible with sustainable agriculture and healthy food is today strongly encouraged. Here, we assessed the biocontrol activity of three cyclic lipopeptides from Bacillus subtilis (mycosubtilin, M; surfactin, S; fengycin, F) and two mixtures (M + S and M + S + F) on wheat against Zymoseptoria tritici, the main pathogen on this crop. Foliar application of these biomolecules at a 100-mg L-1 concentration on the wheat cultivars Dinosor and Alixan, 2 days before fungal inoculation, provided significant reductions of disease severity. The best protection levels were recorded with the M-containing formulations (up to 82% disease reduction with M + S on Dinosor), while S and F treatments resulted in lower but significant disease reductions. In vitro and in planta investigations revealed that M-based formulations inhibit fungal growth, with half-maximal inhibitory concentrations of 1.4 mg L-1 for both M and M + S and 4.5 mg L-1 for M + S + F, thus revealing that the observed efficacy of these products may rely mainly on antifungal property. By contrast, S and F had no direct activity on the pathogen, hence suggesting that these lipopeptides act on wheat against Z. tritici as resistance inducers rather than as biofungicides. This study highlighted the efficacy of several lipopeptides from B. subtilis to biocontrol Z. tritici through likely distinct and biomolecule-dependent modes of action.
Disciplines :
Biotechnology
Author, co-author :
Mejri, Samara; Institut Charles Viollette (EA 7394), ISA, SFR Condorcet FR CNRS 3417, 48 bd Vauban, BP 41290, 59014, Lille, France
Siah, Ali; Institut Charles Viollette (EA 7394), ISA, SFR Condorcet FR CNRS 3417, 48 bd Vauban, BP 41290, 59014, Lille, France
Coutte, François; Institut Charles Viollette (EA 7394), Université de Lille, SFR Condorcet FR CNRS 3417, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France
Magnin-Robert, Maryline; UCEIV-EA 4492, Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, CS 80699, 62228, Calais Cedex, France
Randoux, Béatrice; UCEIV-EA 4492, Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, CS 80699, 62228, Calais Cedex, France
Tisserant, Benoit; UCEIV-EA 4492, Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, CS 80699, 62228, Calais Cedex, France
Krier, François; Institut Charles Viollette (EA 7394), Université de Lille, SFR Condorcet FR CNRS 3417, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France
Jacques, Philippe ; Université de Liège - ULiège > TERRA Research Centre > Microbial, food and biobased technologies ; Institut Charles Viollette (EA 7394), Université de Lille, SFR Condorcet FR CNRS 3417, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France
Reignault, Philippe; UCEIV-EA 4492, Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, CS 80699, 62228, Calais Cedex, France
Halama, Patrice; Institut Charles Viollette (EA 7394), ISA, SFR Condorcet FR CNRS 3417, 48 bd Vauban, BP 41290, 59014, Lille, France. patrice.halama@yncrea.fr
Language :
English
Title :
Biocontrol of the wheat pathogen Zymoseptoria tritici using cyclic lipopeptides from Bacillus subtilis.
INTERREG V SMARTBIOCONTROL NEWBIOPEST Hauts-de-France
Funding text :
Acknowledgements We thank Corentin Duthoo for his technical help during this study and Dr. Gabrielle Chataigné for the HPLC-MS analysis. This research was conducted in the framework of the projects NewBioPest supported by the Hauts-de-France council (France) and both BioProtect and BioScreen supported by INTERREG V SMARTBIOCONTROL (European Union).
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