Glycerol Monolaurate Ameliorated Intestinal Barrier and Immunity in Broilers by Regulating Intestinal Inflammation, Antioxidant Balance, and Intestinal Microbiota.
[en] This study was conducted to investigate the impact of glycerol monolaurate (GML) on performance, immunity, intestinal barrier, and cecal microbiota in broiler chicks. A total of 360 one-day-old broilers (Arbor Acres) with an average weight of 45.7 g were randomly allocated to five dietary groups as follows: basal diet and basal diets complemented with 300, 600, 900, or 1200 mg/kg GML. Samples were collected at 7 and 14 days of age. Results revealed that feed intake increased (P < 0.05) after 900 and 1200 mg/kg GML were administered during the entire 14-day experiment period. Dietary GML decreased (P < 0.05) crypt depth and increased the villus height-to-crypt depth ratio of the jejunum. In the serum and jejunum, supplementation with more than 600 mg/kg GML reduced (P < 0.05) interleukin-1β, tumor necrosis factor-α, and malondialdehyde levels and increased (P < 0.05) the levels of immunoglobulin G, jejunal mucin 2, total antioxidant capacity, and total superoxide dismutase. GML down-regulate (P < 0.05) jejunal interleukin-1β and interferon-γ expression and increased (P < 0.05) the mRNA level of zonula occludens 1 and occludin. A reduced (P < 0.05) expression of toll-like receptor 4 and nuclear factor kappa-B was shown in GML-treated groups. In addition, GML modulated the composition of the cecal microbiota of the broilers, improved (P < 0.05) microbial diversity, and increased (P < 0.05) the abundance of butyrate-producing bacteria. Spearman's correlation analysis revealed that the genera Barnesiella, Coprobacter, Lachnospiraceae, Faecalibacterium, Bacteroides, Odoriacter, and Parabacteroides were related to inflammation and intestinal integrity. In conclusion, GML ameliorated intestinal morphology and barrier function in broiler chicks probably by regulating intestinal immune and antioxidant balance, as well as intestinal microbiota.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Kong, Linglian; Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
Wang, Zhenhua; Center for Mitochondria and Healthy Ageing, College of Life Sciences, Yantai University, Yantai, China
Xiao, Chuanpi ; Université de Liège - ULiège > TERRA Research Centre
Zhu, Qidong; Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
Song, Zhigang; Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
Language :
English
Title :
Glycerol Monolaurate Ameliorated Intestinal Barrier and Immunity in Broilers by Regulating Intestinal Inflammation, Antioxidant Balance, and Intestinal Microbiota.
Natural Science Foundation of Shandong Province National Key Research and Development Program of China
Funding text :
This work was supported by the Natural Science Foundation of Shandong Province (ZR2020MC170), the National Key R&D Program of China (2018YFD0501401-3), and the Shandong Province Agricultural Industry Technology (SDAIT-11-08).
Sánchez-Cordón PJ Montoya M Reis AL Dixon LK. African Swine Fever: A Re-Emerging Viral Disease Threatening the Global Pig Industry. Vet J (2018) 233:41–8. doi: 10.1016/j.tvjl.2017.12.025
Kogut MH. Issues and Consequences of Using Nutrition to Modulate the Avian Immune Response. J Appl Poultry Res (2017) 26(4):605–12. doi: 10.3382/japr/pfx028
Amer SA A-Nasser A Al-Khalaifah HS AlSadek DMM Abdel Fattah DM Roushdy EM et al. Effect of Dietary Medium-Chain α-Monoglycerides on the Growth Performance, Intestinal Histomorphology, Amino Acid Digestibility, and Broiler Chickens’ Blood Biochemical Parameters. Anim (Basel) (2020) 11(1):57. doi: 10.3390/ani11010057
Wang C Shao C Fang Y Wang J Dong N Shan A. Binding Loop of Sunflower Trypsin Inhibitor 1 Serves as a Design Motif for Proteolysis-Resistant Antimicrobial Peptides. Acta Biomater (2021) 124:254–69. doi: 10.1016/j.actbio.2021.01.036
Zhang MS Sandouk A Houtman JCD. Glycerol Monolaurate (GML) Inhibits Human T Cell Signaling and Function by Disrupting Lipid Dynamics. Sci Rep (2016) 6:30225. doi: 10.1038/srep30225
Zhao M Jiang Z Cai H Li Y Mo Q Deng L et al. Modulation of the Gut Microbiota During High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet. mBio (2020) 11(2):e00190–20. doi: 10.1128/mBio.00190-20
Peterson ML Schlievert PM. Glycerol Monolaurate Inhibits the Effects of Gram-Positive Select Agents on Eukaryotic Cells. Biochemistry (2006) 45(7):2387–97. doi: 10.1021/bi051992u
Li Q Estes JD Schlievert PM Duan L Brosnahan AJ Southern PJ et al. Glycerol Monolaurate Prevents Mucosal SIV Transmission. Nature (2009) 458(7241):1034–8. doi: 10.1038/nature07831
Fortuoso BF Galli GM Griss LG Armanini EH Silva AD Fracasso M et al. Effects of Glycerol Monolaurate on Growth and Physiology of Chicks Consuming Diet Containing Fumonisin. Microb Pathog (2020) 147:104261. doi: 10.1016/j.micpath.2020.104261
Welch JL Xiang J Okeoma CM Schlievert PM Stapleton JT. Glycerol Monolaurate, an Analogue to a Factor Secreted by, Is Virucidal Against Enveloped Viruses, Including HIV-1. mBio (2020) 11(3):e00686–20. doi: 10.1128/mBio.00686-20
Fortuoso BF Dos Reis JH Gebert RR Barreta M Griss LG Casagrande RA et al. Glycerol Monolaurate in the Diet of Broiler Chickens Replacing Conventional Antimicrobials: Impact on Health, Performance and Meat Quality. Microb Pathog (2019) 129:161–7. doi: 10.1016/j.micpath.2019.02.005
Valentini J Da Silva AS Fortuoso BF Reis JH Gebert RR Griss LG et al. Chemical Composition, Lipid Peroxidation, and Fatty Acid Profile in Meat of Broilers Fed With Glycerol Monolaurate Additive. Food Chem (2020) 330:127187. doi: 10.1016/j.foodchem.2020.127187
Silva VDO Pereira LJ Pasetto S da Silva MP Meyers JC Murata RM. Effects of Monolaurin on Oral Microbe-Host Transcriptome and Metabolome. Front Microbiol (2018) 9:2638. doi: 10.3389/fmicb.2018.02638
Pan D Yu Z. Intestinal Microbiome of Poultry and its Interaction With Host and Diet. Gut Microbes (2014) 5(1):108–19. doi: 10.4161/gmic.26945
Mo Q Fu A Deng L Zhao M Li Y Zhang H et al. High-Dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota Without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights Into Its Antimicrobial Potential. Nutrients (2019) 11(9):1981. doi: 10.3390/nu11091981
Liu T Tang J Feng F. Glycerol Monolaurate Improves Performance, Intestinal Development, and Muscle Amino Acids in Yellow-Feathered Broilers via Manipulating Gut Microbiota. Appl Microbiol Biotechnol (2020) 104(23):10279–91. doi: 10.1007/s00253-020-10919-y
Ren C Wang Y Lin X Song H Zhou Q Xu W et al. A Combination of Formic Acid and Monolaurin Attenuates Enterotoxigenic Induced Intestinal Inflammation in Piglets by Inhibiting the NF-κb/MAPK Pathways With Modulation of Gut Microbiota. J Agric Food Chem (2020) 68(14):4155–65. doi: 10.1021/acs.jafc.0c01414
Pham VH Kan L Huang J Geng Y Zhen W Guo Y et al. Dietary Encapsulated Essential Oils and Organic Acids Mixture Improves Gut Health in Broiler Chickens Challenged With Necrotic Enteritis. J Anim Sci Biotechnol (2020) 11:18. doi: 10.1186/s40104-019-0421-y
Surai PF Kochish II Kidd MT. Redox Homeostasis in Poultry: Regulatory Roles of NF-κb. Antioxid (Basel) (2021) 10(2):186. doi: 10.3390/antiox10020186
Lugrin J Rosenblatt-Velin N Parapanov R Liaudet L. The Role of Oxidative Stress During Inflammatory Processes. Biol Chem (2014) 395(2):203–30. doi: 10.1515/hsz-2013-0241
Jackman JA Hakobyan A Zakaryan H Elrod CC. Inhibition of African Swine Fever Virus in Liquid and Feed by Medium-Chain Fatty Acids and Glycerol Monolaurate. J Anim Sci Biotechnol (2020) 11(1):114. doi: 10.1186/s40104-020-00517-3
Baltic B Starčević M Djordjevic J Mrdović B Markovic R. Importance of Medium Chain Fatty Acids in Animal Nutrition. IOP Conf Series: Earth Environ Sci (2017) 85:12048. doi: 10.1088/1755-1315/85/1/012048
Jackman JA Boyd RD Elrod CC. Medium-Chain Fatty Acids and Monoglycerides as Feed Additives for Pig Production: Towards Gut Health Improvement and Feed Pathogen Mitigation. J Anim Sci Biotechnol (2020) 11:44. doi: 10.1186/s40104-020-00446-1
Abdollahi MR Zaefarian F Ravindran V. Feed Intake Response of Broilers: Impact of Feed Processing. Anim Feed Sci Technol (2018) 237:154–65. doi: 10.1016/j.anifeedsci.2018.01.013
Pié S Lallès JP Blazy F Laffitte J Sève B Oswald IP. Weaning Is Associated With an Upregulation of Expression of Inflammatory Cytokines in the Intestine of Piglets. J Nutr (2004) 134(3):641–7. doi: 10.1093/jn/134.3.641
Liu T Li C Zhong H Feng F. Dietary Medium-Chain α-Monoglycerides Increase BW, Feed Intake, and Carcass Yield in Broilers With Muscle Composition Alteration. Poult Sci (2021) 100(1):186–95. doi: 10.1016/j.psj.2020.09.056
Hong SM Hwang JH Kim IH. Effect of Medium-Chain Triglyceride (MCT) on Growth Performance, Nutrient Digestibility, Blood Characteristics in Weanling Pigs. Asian-Australas J Anim Sci (2012) 25(7):1003–8. doi: 10.5713/ajas.2011.11402
Masmeijer C Rogge T van Leenen K De Cremer L Deprez P Cox E et al. Effects of Glycerol-Esters of Saturated Short- and Medium Chain Fatty Acids on Immune, Health and Growth Variables in Veal Calves. Prev Vet Med (2020) 178:104983. doi: 10.1016/j.prevetmed.2020.104983
Nishimura Y Moriyama M Kawabe K Satoh H Takano K Azuma Y-T et al. Lauric Acid Alleviates Neuroinflammatory Responses by Activated Microglia: Involvement of the GPR40-Dependent Pathway. Neurochem Res (2018) 43(9):1723–35. doi: 10.1007/s11064-018-2587-7
Sivinski SE Mamedova LK Rusk RA Elrod CC Swartz TH McGill JM et al. Development of an Macrophage Screening System on the Immunomodulating Effects of Feed Components. J Anim Sci Biotechnol (2020) 11:89. doi: 10.1186/s40104-020-00497-4
Sun S-C. The non-Canonical NF-κb Pathway in Immunity and Inflammation. Nat Rev Immunol (2017) 17(9):545–58. doi: 10.1038/nri.2017.52
Lee JY Zhao L Youn HS Weatherill AR Tapping R Feng L et al. Saturated Fatty Acid Activates But Polyunsaturated Fatty Acid Inhibits Toll-Like Receptor 2 Dimerized With Toll-Like Receptor 6 or 1. J Biol Chem (2004) 279(17):16971–9. doi: 10.1074/jbc.M312990200
Jiang J Qi L Lv Z Jin S Wei X Shi F. Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage Through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens. Antioxid (Basel) (2019) 8(12):575. doi: 10.3390/antiox8120575
Shang Y Regassa A Kim JH Kim WK. The Effect of Dietary Fructooligosaccharide Supplementation on Growth Performance, Intestinal Morphology, and Immune Responses in Broiler Chickens Challenged With Salmonella Enteritidis Lipopolysaccharides. Poult Sci (2015) 94(12):2887–97. doi: 10.3382/ps/pev275
Cromarty R Archary D. Inflammation, HIV, and Immune Quiescence: Leveraging on Immunomodulatory Products to Reduce HIV Susceptibility. AIDS Res Treat (2020) 2020:8672850. doi: 10.1155/2020/8672850
Latha S Chaudhary S Ray SR. Hydroalcoholic Extract of Stevia Rebaudiana Bert. Leaves and Stevioside Ameliorates Lipopolysaccharide Induced Acute Liver Injury in Rats. BioMed Pharmacother (2017) 95:1040–50. doi: 10.1016/j.biopha.2017.08.082
Lucas K Maes M. Role of the Toll Like Receptor (TLR) Radical Cycle in Chronic Inflammation: Possible Treatments Targeting the TLR4 Pathway. Mol Neurobiol (2013) 48(1):190–204. doi: 10.1007/s12035-013-8425-7
Wang W Li Z Han Q Guo Y Zhang B D’Inca R. Dietary Live Yeast and Mannan-Oligosaccharide Supplementation Attenuate Intestinal Inflammation and Barrier Dysfunction Induced by Escherichia Coli in Broilers. Br J Nutr (2016) 116(11):1878–88. doi: 10.1017/S0007114516004116
Ott SJ Musfeldt M Wenderoth DF Hampe J Brant O Fölsch UR et al. Reduction in Diversity of the Colonic Mucosa Associated Bacterial Microflora in Patients With Active Inflammatory Bowel Disease. Gut (2004) 53(5):685–93. doi: 10.1136/gut.2003.025403
Olejniczak-Staruch I Ciążyńska M Sobolewska-Sztychny D Narbutt J Skibińska M Lesiak A. Alterations of the Skin and Gut Microbiome in Psoriasis and Psoriatic Arthritis. Int J Mol Sci (2021) 22(8):3998. doi: 10.3390/ijms22083998
Islam MR Lepp D Godfrey DV Orban S Ross K Delaquis P et al. Effects of Wild Blueberry (Vaccinium Angustifolium) Pomace Feeding on Gut Microbiota and Blood Metabolites in Free-Range Pastured Broiler Chickens. Poult Sci (2019) 98(9):3739–55. doi: 10.3382/ps/pez062
Yu M Mu C Zhang C Yang Y Su Y Zhu W. Marked Response in Microbial Community and Metabolism in the Ileum and Cecum of Suckling Piglets After Early Antibiotics Exposure. Front Microbiol (2018) 9:1166. doi: 10.3389/fmicb.2018.01166
Shi S Qi Z Jiang W Quan S Sheng T Tu J et al. Effects of Probiotics on Cecal Microbiome Profile Altered by Duck Escherichia Coli 17 Infection in Cherry Valley Ducks. Microb Pathog (2020) 138:103849. doi: 10.1016/j.micpath.2019.103849
Arpaia N Campbell C Fan X Dikiy S van der Veeken J deRoos P et al. Metabolites Produced by Commensal Bacteria Promote Peripheral Regulatory T-Cell Generation. Nature (2013) 504(7480):451–5. doi: 10.1038/nature12726
Zhou Y Wang Y Quan M Zhao H Jia J. Gut Microbiota Changes and Their Correlation With Cognitive and Neuropsychiatric Symptoms in Alzheimer’s Disease. J Alzheimers Dis (2021) 81(2):583–95. doi: 10.3233/JAD-201497
Jiang Z Zhao M Zhang H Li Y Liu M Feng F. Antimicrobial Emulsifier-Glycerol Monolaurate Induces Metabolic Syndrome, Gut Microbiota Dysbiosis, and Systemic Low-Grade Inflammation in Low-Fat Diet Fed Mice. Mol Nutr Food Res (2018) 62(3):1700547. doi: 10.1002/mnfr.201700547
