Article (Scientific journals)
Proteolytic Breakdown Of Gliadin By Enterococcus Faecalis Isolated From Tunisian Fermented Dough
M'Hir, S.; Aldric, Jean-Marc; El Mejdoub, Thami et al.
2008In World Journal of Microbiology and Biotechnology, 24 (12), p. 2775-2781
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Keywords :
Enterococcus; gliadin; proteolysis; screening; wheat liquid dough
Abstract :
[en] The aim of this work was to select strains with proteolytic activity on wheat gliadin, among lactic acid bacteria, previously isolated from Tunisian fermented wheat dough. Hydrolysis of gliadin, as sole nitrogen source, in an agar medium was visualized by a clear zone surrounding colonies. The increase in absorbance due to gliadin breakdown was measured spectrophotometrically using Ophthaldialdehyde (OPA) on Gliadin Glucose Broth medium. Fermented liquid dough inoculated with individual selected Enterococcus faecalis, showed a decrease of the gliadin concentration from 45 g/kg to 18 g/kg determined by sandwich ELISA test (R-7001). Only the enterococci strains show an hydrolysis of gliadin proteins. Strains showing proteolytic activity are gaining more and more importance in cereal based fermented foods and may help to reduce gliadin involved in coeliac disease.
Disciplines :
Biotechnology
Microbiology
Author, co-author :
M'Hir, S.
Aldric, Jean-Marc ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
El Mejdoub, Thami ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Destain, Jacqueline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Mejri, M.
Hamdi, M.
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Language :
English
Title :
Proteolytic Breakdown Of Gliadin By Enterococcus Faecalis Isolated From Tunisian Fermented Dough
Publication date :
2008
Journal title :
World Journal of Microbiology and Biotechnology
ISSN :
0959-3993
eISSN :
1573-0972
Publisher :
Springer, New York, United States - New York
Volume :
24
Issue :
12
Pages :
2775-2781
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 November 2009

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