[en] Affinity-maturation and Ab class switches occur in lymphoid germinal centers (GCs), in which differentiation and maintenance depend on lymphotoxin (LT) signaling and include differentiation of follicular dendritic cells (FDCs). The events leading to FDC and GC maturation are poorly defined. Using several approaches of functional genomics, we enumerated transcripts affected in mice by suppressing LT beta receptor (LT beta R) signaling and/or overrepresented in FDC-enriched GC isolates. Protein expression analysis of 3 of 12 genes both enriched in FDCs and down-regulated by LT beta R signaling suppression validated them as FDC markers. Functional analysis of one of these three, clusterin, suggests a role as an FDC-derived trophic factor for GC B cells. Hence, the set of genes presented in this study includes markers emanating from LT beta R signaling and transcripts relevant to GC and FDC function.
Disciplines :
Immunology & infectious disease
Author, co-author :
Huber, Christoph; University Hospital of Zurich > Insitute of Neuropathology
Thielen, Caroline ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie Humaine
Seeger, Harald; University Hospital of Zurich > Insitute of Neuropathology
Schwarz, Petra; University Hospital of Zurich > Insitute of Neuropathology
Montrasio, Fabio; University Hospital of Zurich > Insitute of Neuropathology
Wilson, Mark
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Fu, Yang-Xin
Miele, Gino; University Hospital of Zurich > Insitute of Neuropathology
Aguzzi, Adriano; University Hospital of Zurich > Insitute of Neuropathology
Language :
English
Title :
Lymphotoxin-beta receptor-dependent genes in lymph node and follicular dendritic cell transcriptomes
Publication date :
01 May 2005
Journal title :
Journal of Immunology
ISSN :
0022-1767
eISSN :
1550-6606
Publisher :
Amer Assoc Immunologists, Bethesda, United States - Maryland
Volume :
174
Issue :
9
Pages :
5526-5536
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
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