[en] In the present study, we have established and validated a radioenzyme assay which permits us to quantify progesterone metabolism in the chicken brain. Progesterone metabolism was then studied in five brain areas obtained by microdissection from the telencephalon (part of the lobus paraolfactorius immediately rostral to the preoptic area), the preoptic area, and the hypothalamus. Three metabolites of progesterone were produced in large amounts in these brain regions and were quantified in this study: 5 beta-pregnane-3,20-dione (5 beta-DHP) as well as its metabolite 3 alpha-hydroxy-5 beta-pregnane-20-one (5 beta,3 alpha-ol) and 5 alpha-pregnane-3,20-dione (5 alpha-DHP). The unmetabolized progesterone was also recovered and quantified. The 5 beta-reduction of progesterone (production of 5 beta-DHP and 5 beta,3 alpha-ol) was very active but its 5 alpha-reduction (production of 5 alpha-DHP) was almost absent in the lobus paraolfactorius. An opposite pattern of metabolism was found in the preoptic area and the hypothalamus (higher 5 alpha- but lower 5 beta-reductase activity). The changes in progesterone metabolism in these brain areas were then studied in groups of hens submitted to induced egg laying stop and molting. A significant decrease in progesterone 5 alpha-reduction was found in the median hypothalamus of hens during the period of molt. Simultaneously, the experimental procedures induced significant decreases in the production of 5 beta-DHP by the lobus paraolfactorius, anterior, and medial hypothalamus but induced a significant increase in the production of this metabolite in the preoptic area. These changes are likely to be involved in the control of reproductive functions including sexual behavior and secretion of luteinizing hormone-releasing hormone, and a number of possible causal mechanisms are presented. These should now be tested experimentally especially in view of the very limited information which is now available on the biological effects of the metabolites of progesterone.
Balthazart, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Verheyen, G.
Schumacher, M.
Decuypere, E.
Language :
English
Title :
Changes in Progesterone Metabolism in the Chicken Hypothalamus During Induced Egg Laying Stop and Molting
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Bibliography
Bahr, Johnson (1984) Regulation of the follicular hierarchy and ovulation. J. Exp. Zool 232:495-500.
Balthazart (1983) Hormonal correlates of behaviour. Avian Biology , D.S. Farner, J.R. King, K.C. Parker, Academic Press, New York; 7:221-265.
Balthazart, Schumacher (1985) Role of testosterone metabolism in the activation of sexual behaviour. Comparative Neurobiology , R. Gilles, J. Balthazart, Springer-Verlag, Berlin; 121-140.
Barfield, Rubin, Glaser, Davis (1983) Sites of action of ovarian hormones in the regulation of oestrous responsiveness in rats. Hormones and Behaviour in Higher Vertebrates , J. Balthazart, E. Prove, R. Gilles, Springer-Verlag, Berlin; 2-17.
Berry, Brake (1985) Comparison of parameters associated with molt induced by fasting zinc and low dietary sodium in caged layers. Poultry Science 64:2027-2036.
Bradford (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem 72:248-254.
Brake, Thaxton, Benton (1979) Physiological changes in caged layers during a forced molt. 3. Plasma thyroxine, plasma triiodo thyronine, adrenal cholesterol, and total adrenal steroids. Poult. Sci 58:1345-1350.
Czaja, Goldfoot, Karavolas (1974) Comparative facilitation and inhibition of lordosis in the guinea pig with progesterone, 5α-pregnane-3,20-dione, or 3α-hydroxy-5α-pregnan-20-one. Horm. Behav 5:261-274.
Decuypere, Verheyen (1986) Physiological basis of moulting and tissue regeneration. World's Poultry Science Journal 42:56-68.
Decuypere, Verheyen, Kuhn, Balthazart (1984) Shortening of the Egg Laying Stop Period with LH-RH Injection in Molting Hens. XVIIth World's Poultry Congress, Finland; .
Delville, Balthazart (1987) Hormonal control of female sexual behavior in the Japanese quail. Horm. Behav 21:288-309.
Deviche, Delville, Balthazart (1987) Central and peripheral metabolism of 5α-dihydrotestosterone in the male Japanese quail: Biochemical characterization and relationship with reproductive behavior. Brain Res 421:105-116.
Etches, Williams, Rzasa (1984) Effect of corticosterone and dietary changes in the hen on ovarian function, plasma LH and ste roids and the response to exogenous LHRH. J. Reprod. Fert 70:121-130.
Feder (1981) Essential of steroid structure, nomenclature, reactions, biosynthesis and measurements. Neuroendocrinology of Reproduction. Physiology and Behavior , N.T. Adler, Plenum, New York; 19-63.
Gasc, Baulieu (1988) Regulation by estradiol of the progesterone receptor in the hypothalamus and pituitary: An immunohistochemical study in the chicken. Endocrinology 122:1357-1365.
Gibson, Cheng (1979) Neural mediation of estrogen-dependent courtship behavior in female ring doves. J. Comp. Physiol. Psychol 93:855-867.
Gildersleeve, Satterlee, Johnson, Scott (1982) Theeffect of forced molt treatments on selected steroids in hens. Poult. Sci 61:2362-2369.
Himeno, Tanabe (1957) Mechanism of molting in hen. Poultry Science 36:835-842.
Johnson, Van Tienhoven (1980) Hypothalamo-hypophyseal sensitivity to central injections of progesterone and LH-RH in the laying and molting hen. Adv. Physiol. Sci 33:99-107.
Karavolas, Bertics, Hodges, Rudie (1984) Progesterone processing by neuroendocrine structures. Metabolism of Hormonal Steroids in the Neuroendocrine Structures , L. Martini, Raven Press, New York; 13:149-170.
Karavolas, Hodges, O'Brien (1976) Uptake of 3H-progesterone and 3H-5α-dihydroprogesterone by rat tissue in vivo and analysis of accumulated radioactivity: Accumulation of 5α-dihydroprogesterone by pituitary and hypothalamic tissues. Endocrinology 98:164-175.
Karavolas, Hodges, O'Brien, McKenzie (1979) In vivo uptake of (3H) progesterone and (su3H)-5α-dihydroprogesterone by rat brain and pituitary and effects of estradiol and time: Tissue concentration of progesterone itself or specific metabolites. Endocrinology 104:1418-1425.
Kawashima, Kamiyoshi, Tanaka (1978) A cytoplasmic progesterone receptor in hen pituitary and hypothalamic tissue. Endocrinology 102:1207-1273.
Kawashima, Kamiyoshi, Takana (1979) Cytoplasmatic progesterone receptor concentrations in the hen hypothalamus and pituitary: Difference between laying and non laying hens and changes during the ovulatory cycle. Biol. Reprod 20:581-585.
Luck, Scanes (1979) The relationship between reproductive activity and blood calcium in the calcium-deficient hen. Brit. Poult. Sci 20:559-564.
Massa, Martini (1972) Interference with the 5α-reductase system Hormones and antagonists. Gynecologic and Obstetric Investigation 2:253-270.
Nakamura, Tanabe (1974) In vitro metabolism of steroid hormones by chicken brain. Acta Endocrinol 75:410-416.
Noble (1972) The effects of estrogen and progesterone, on copulation in female quail (Coturnix coturnix japonica) housed in continuous dark. Horm. Behav 3:199-204.
Pethes, Szelenyi, Peczely (1982) Changes in the plasma concentrations of thyroid hormones and sexual steroids during forced molt of male and female domestic chickens. Acta Vet. Acad. Sci. Hung 30:193-201.
Ralph, Fraps (1960) Induction of ovulation in the hen by injection of progesterone into the brain. Endocrinology 66:269-272.
Roselli, Snipes (1984) Progesterone 5α-reductase in mouse brain. Brain Res 305:197-202.
Rubin, Barfield (1983) Induction of estrous behavior in ovariectomized rats by sequential replacement of estrogen and progester one to the ventromedial hypothalamus. Neuroendocrinology 37:218-224.
Schenborn, Karavolas (1983) Hypothalamic progestin receptors: Evidence for two classes of high affinity binding sites. Endocrinology 112:121-138.
Schumacher, Balthazart (1986) Testosterone-induced brain aromatase is sexually dimorphic. Brain Res 370:285-293.
Schumacher, Contenti, Balthazart (1983) Testosterone metabolism in discrete areas of the hypothalamus and adjacent brain regions of male and female Japanese quail (Coturnix coturnix japonica). Brain Res 278:337-340.
Schumacher, Contenti, Balthazart (1984) Partial characterization of testosteronemetabolizing enzymes in the quail brain. Brain Res 305:51-59.
Sharp, Massa (1980) Conversion of progesterone 5a- and 5(3-reduced metabolites in the brain of the hen and its potential role in the induction of the preovulatory release of luteinizing hormone. J. Endocrinol 86:459-464.
Steimer, Hutchison (1981) Metabolic control of the behavioral action of androgens in the dove brain: Testosterone inactivation by 5(3-reduction. Brain Res 209:189-204.
Sterling, Gasc, Sharp, Renoir, Tuohimaa, Baulieu (1987) The distribution of nuclear progesterone receptor in the hypothalamus and forebrain of the domestic hen. Cell Tissue Res 248:201-205.
Sterling, Gasc, Sharp, Tuohimaa, Baulieu (1984) Absence of nuclear progesterone receptor in LH releasing hormone neurones in laying hens. Journal of Endocrinology 102:R5-R7.
Stupaicka, Massa, Zanisi, Martini (1977) Role of anterior pituitary and hypothalamic metabolism of progesterone in the control of gonadotropin secretion. Prog. Reprod. Biol 2:88-95.
Tanabe, Himeno, Nozaki (1957) Thyroid and ovarian function in relation to molting in the hen. Endocrinology 61:661-666.
Tanabe, Ogawa, Nakamura (1981) The effect of short-term starvation on pituitary and plasma LH, plasma estradiol and progesterone, and on pituitary response to LH-RH in the laying hen (Gallus domesticus). Gen. Comp. Endocrinol 43:392-398.
Van Tienhoven (1980) Neuroendocrinology of avian reproduction with special emphasis on the reproductive cycle of the fowl (Gallus domesticus). World's Poultry Science Journal 37:156-176.
Verheyen, Decuypere, Chiasson, Vervloesem, Kuhn, Michels (1987) Effect of exogenous oestradiol in relation to the cessation of egg laying induced by different moulting methods. J. Reprod. Fert 81:13-21.
Verheyen, Decuypere, Kuhn, Fontaine, De Groote (1983) Arrêt de la ponte par induction chez la poule. Effet de différentes méthodes sur certains paramètres de production et sur les concentrations en hormones thyroidiennes, en prolactine, en Ca, P, Na et en proteines dans le sérum sanguin. Rev. Agric 36:1535-1559.
Verheyen, Decuypere, Kuhn, Herremans (1986) Dissociation of the effect of thyroxine and triiodothyronine in relation to the halt egg laying and molt in hens. Arch. Exp. Vet. Med 40:250-259.
Verheyen, Decuypere, Kuhn, Balthazart (1986) Hormonal Sensitivity at the Hypothalamo-Pituitary-Ovarian Axis during a Forced Moult in Hens. Annual Conference of the Society for the Study of Fertility, Abstract 53A; .
Wood-Gush, Gilbert (1973) Some hormones involved in the nesting behaviour of hens. Anim. Behav 21:98-103.
Zanisi, Martini (1975) Effects of progesterone metabolites on gonadotrophin secretion. J. Steroid Biochem 6:1021-1023.
Zanisi, Messi, Martini (1984) Physiological role of 5α-reduced metabolites of progesterone. Metabolism of Hormonal Steroids in the Neuroendocrine Structures , L. Martini, Raven Press, New York; 13:171-183.
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