[en] We evaluated the analytical and clinical performances of a new radial partition immunoassay for measuring the mass concentration of creatine kinase (CK)-MB in serum. All pipetting, washes, incubations and data reduction were performed in 8 min by the Stratus (Dade) fluorometric analyzer. Within-assay and between-assay CVs were respectively 5.5% and 8.4% at 21 micrograms/L, and 4.2% and 3.4% at 48 micrograms/L. Assaying serial dilutions of serum samples with high CK-MB concentrations demonstrated excellent linearity. Results of the Stratus technique correlated well (n = 115, r = 0.98) with those of the Tandem-E CKMB II assay. There was no interference from hemolysis, bilirubin, rheumatoid factor, or added CK-MM (up to 3500 U/L); consequently, CK-MB can be determined in undiluted serum, even in the presence of high total CK activity. The mean CK-MB concentration in 105 blood donors was 1.9 (SD 1.3) micrograms/L. For seven myocardial infarction patients who received prompt fibrinolytic therapy, the mean CK-MB concentration was 4.5 (SD 1.8) micrograms/L at admission, and maximum concentrations, 119 (SD 94) micrograms/L, were recorded 16 h later. CK-MB returned to concentrations less than 10 micrograms/L within 72 h.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Chapelle, Jean-Paul ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
El Allaf, M.
Language :
English
Title :
Automated quantification of creatinine kinase MB isoenzyme in serum by radial partition immunoassay, with use of the Stratus analyzer
Publication date :
January 1990
Journal title :
Clinical Chemistry
ISSN :
0009-9147
eISSN :
1530-8561
Publisher :
American Association for Clinical Chemistry, Washington, United States - District of Columbia
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Lee TH, Goldman L. Serum enzyme assays in the diagnosis of acute myocardial infarction: recommendations based on a quantitative analysis [Review]. Ann Intern Med 1986;105:221-33.
Lott JA, Stang JM. Serum enzymes and isoenzymes in the diagnosis of myocardial ischemia and necrosis [Review]. Clin Chem 1980;26:1241-50.
Rugg JA, Rigi CT, Leung K, et al. Radial partition immunoassay applied to automated quantification of human choriogonadotropin with use of two monoclonal antibodies. Clin Chem 1986;32:1844-8.
Stahlschmidt GE, Smith CH, Miller RE, et al. Stratus fluorometric enzyme immunoassay system evaluated for determination of total thyroxin [Tech Brief]. Clin Chem 1988;34:433-4.
Vernet M, Revenant M-C, Bied A, et al. Rapid determination of ferritin in serum by the "Stratus" fluoroenzymoimmunometric assay [Tech Brief]. Clin Chem 1989;35:672-3.
Rugg JA, Flaa CW, Dawson SR, et al. Automated quantification of thyrotropin by radial partition immunoassay. Clin Chem 1988;34:118-22.
Sapin R, Gasser F, Schlienger JL. "Stratus" fluorometric enzyme immunoassay system evaluated for determination of free thyroxin [Tech Brief]. Clin Chem 1988;34:2159-60.
Giegel JL, Brotherton MM, Cronin P, et al. Radial partition immunoassay. Clin Chem 1982;28:1894-8.
Hall M, Neisler M, Johnson JT. Immunoenzymometric assay of creatine kinase with monoclonal antibodies to the MB isoenzyme compared with electrophoresis. Clin Chem 1988;34:2539-42.
Reed AH, Henry RJ, Mason WB. Influence of statistical method used on the resulting estimate of normal range. Clin Chem 1971;17:275-84.
Golf SW, Temme H, Kempf KD, et al. Systemic short-term fibrinolysis with high-dose streptokinase in acute myocardial infarction: time course of biochemical parameters. J Clin Chem Clin Biochem 1984;22:723-9.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.