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Typing of Bovine Attaching and Effacing Escherichia Coli by Multiplex in Vitro Amplification of Virulence-Associated Genes
China, B.; Pirson, V.; Mainil, Jacques
1996In Applied and Environmental Microbiology, 62 (9), p. 3462-3465
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Abstract :
[en] Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
China, B.
Pirson, V.
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Typing of Bovine Attaching and Effacing Escherichia Coli by Multiplex in Vitro Amplification of Virulence-Associated Genes
Publication date :
September 1996
Journal title :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
62
Issue :
9
Pages :
3462-3465
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 November 2009

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