[en] BACKGROUND: Microsporum-canis-infected cats, especially the asymptomatic infected ones, are mainly responsible for the zoonotic disease. The important variability of the clinical signs in cats is poorly understood. Recently, a 31.5-kD keratinolytic subtilase was found to be a putative virulence factor. OBJECTIVE: To investigate the possible relationship between the clinical status of dermatophytic cats and the production of the keratinase. METHODS: Seven M. canis strains isolated either from clinically affected, asymptomatic infected or mechanical carrier cats were tested for the in vitro production of the enzyme. The immunohistochemical detection of the enzyme was also assessed in skin biopsies of 4 symptomatic and 7 asymptomatic naturally infected cats. RESULTS: All the strains produced in vitro a 31.5-kD keratinolytic subtilase. The enzyme was present in all but 1 of the infected cats. CONCLUSION: The production of the keratinase is not a factor directly responsible for the clinical picture seen in M.-canis-infected cats.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Nikkels, Arjen ; Centre Hospitalier Universitaire de Liège - CHU > Dermatologie
Pierard, Gérald ; Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
The in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected cats.
Lunder M, Lunder M: Is Microsporum canis infection about to become a serious dermatological problem? Dermatology 1992;184:87-89.
Muller GH, Kirk RW, Scott DW: Fungal diseases; in Dyson J (ed): Small Animal Dermatology. Philadelphia, Saunders 1989, pp 295-346.
Mignon BR, Losson BJ: Prevalence and characterization of Microsporum canis carriage in cats. J Med Vet Mycol 1997;35:249-256.
Simpanya MF, Baxter M: Multiple proteinases from two Microsporum species. J Med Vet Mycol 1996;34:31-36.
Simpanya MF, Baxter M: Partial characterization of proteolytic enzymes of Microsporum cookei. Mycoses 1996;39:279-282.
Mignon B, Swinnen M, Bouchara JP, Hofinger M, Nikkels A, Piérard G, Gerday C, Losson B: Purification and characterization of a 31.5-kD keratinolytic subtilase from Microsporum cams and evidence of its secretion in naturally infected cats. Med Mycol, submitted.
Raubitschek F: Mechanical versus chemical keratolysis by dermatophytes. Sabouraudia 1961;1:87-90.
Nikkels AF, Delvenne P, Debrus S, Sadzot- Delvaux C, Piette J, Rentier B, Piérard GE: Distribution of varicella-zoster virus gpI and gpII and corresponding genome sequences in the skin. J Med Virol 1995;46:91-96.
Chermette R, Ferreiro L, Huang W, Larcher G, Polack B, Mialot M: Microsporum canis in pet carnivores: Focus on pseudomycetomas and enzymatic activity. Proc 13th ISHAM Congr, Parma, 1997, p 44.
Sparkes AH, Stokes CR, Gruffyd-Jones TJ: Experimental Microporum canis infection in cats: Correlation between immunological and clinical observations. J Med Vet Mycol 1995; 33:177-184.
DeBoer DJ, Moriello KA: Humoral and cellular immune responses to Microsporum canis in naturally occurring feline dermatophytosis. J Med Vet Mycol 1993;31:121-132.
Grappel SF, Blank F: Role of keratinases in dermatophytosis. I. Immune responses of guinea pigs infected with Trichophyton menlagrophytes and guinea pigs immunized with keratinases. Dermatologica 1972;45:245-255.
Lee KH, Lee JB, Song DH: Detection of circulating antibodies to purified keratinolytic proteinase in sera from guinea pigs infected with Microsporum canis by enzyme-linked immunosorbent assay. Arch Dermatol Res 1988;280:45-49.
Takiuchi IY, Tagaki SH, Negi M: Partial characterization of the extracellular keratinase from Microsporum canis. Sabouraudia 1984; 22:219-224.