[en] BACKGROUND: The number of eosinophils is increased in the mucosae of the digestive and the respiratory tracts in Crohn disease, even clinically quiescent. The mechanisms underlying this panmucosal eosinophilia are unknown. METHODS: The response of blood eosinophils to various chemotactic agents was assessed in 15 patients with clinically quiescent Crohn disease. The results were compared with 15 healthy controls. After purification, eosinophils were placed in Boyden microchambers and the chemotactic effect of PAF (10(-7) M), RANTES (50 ng/ml), IL-5 (0-20 ng/ml), IL-8 (0-50 ng/ml), Eotaxin (0-50 ng/ml) was evaluated. The number of eosinophils in induced sputum of these Crohn disease patients and controls was also assessed and the correlation between chemotaxis and eosinophil count in induced sputum was studied. RESULTS: PAF and RANTES induced a chemotactic effect both in Crohn disease patients and controls. The chemotactic index was significantly higher in Crohn than controls for PAF (2.09+/-0.24 versus 1.37+/-0.14; P < 0.05) but not RANTES. With IL-5, IL-8 and Eotaxin, there was no detectable chemotactic effect in controls while in Crohn, we observed a significant dose-dependent chemotactic effect. Furthermore, with Eotaxin 50 ng/ml, the chemotactic index was significantly higher in Crohn disease patients than controls (2.42+/-0.18 versus 1.56+/-0.28; P < 0.05). A significant increase in sputum eosinophil count and a significant decrease in sputum macrophage count in Crohn disease were observed. However, there was no correlation between eosinophil chemotaxis and sputum eosinophil count in individual patients. CONCLUSION: There is an increased response of blood eosinophils to various chemotactic agents, mainly PAF and Eotaxin, in clinically quiescent Crohn disease. This may participate in the mucosal infiltration by eosinophils in this disease.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
Denis, M. A.
Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Malaise, Michel ; Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
Belaiche, Jacques ; Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Louis, Edouard ; Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Language :
English
Title :
Increased Response of Blood Eosinophils to Various Chemotactic Agents in Quiescent Crohn Disease
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Bibliography
Fiocchi C. (1998) Inflammatory bowel disease: Etiology and pathogenesis. Gastroenterology 115:182-205.
Bischoff S.C., Wedemeyer J., Herrmann A., Meier P.N., Trautwein C., Cetin Y. (1996) Quantitative assessment of intestinal eosinophils and mast cells in inflammatory bowel disease. Histopathology 28:1-13.
Dubucquoi S., Janin A., Klein O., Desreumaux P., Quandalle P., Cortot A. (1995) Activated eosinophils and interleukin 5 expression in early recurrence of Crohn's disease. Gut 37:242-246.
D'Haens G., Geboes K., Peeters M., Baert F., Penninckx F., Rutgeerts P. (1998) Early lesions of recurrent Crohn's disease caused by infusion of intestinal contents in excluded ileum. Gastroenterology 114:262-267.
D'Haens G., Colpaert S., Peeters M., Baert F., Penninckx F., Rutgeerts P. (1998) The presence and severity of neural inflammation predict severe postoperative recurrence of Crohn's disease. Gastroenterology 114.
Louis E., Louis R., Shute J., Lau L., Franchimont D., Lamproye A. (1999) Bronchial eosinophilic infiltration in Crohn's disease in the absence of pulmonary disease. Clin Exp Allergy 29:660-666.
Wardlaw A.J., Walsh G., Symon A. (1994) Mechanisms of eosinophil and basophil migration. Allergy 49:797-807.
Kald B., Olaison G., Sjodahl R., Tagesson C. (1990) Novel aspects of Crohn's disease: Increased content of platelet activating factor in ileal and colonic mucosa. Digestion 46:199-204.
Fromont Hankard G., Brousse N., Cézard J.P., Emilie D., Peuchmaur M. (1997) In situ interleukin 5 gene expression in pediatric Crohn's disease. J Pediatr Gastroenterol Nutr 24:568-572.
Mitsuyama K., Toyonaga A., Sasaki E., Watanabe K., Tateishi H., Nishiyama T. (1994) IL-8 as an important chemoattractant for neutrophils in ulcerative colitis and Crohn's disease. Clin Exp Immunol 96:432-436.
Berrebi D., Banerjee A., Paris R., Potet F., Aigrin Y., Emilie D. (1997) In situ RANTES and interferon-γ gene expression in pediatric small bowel Crohn's disease. J Pediatr Gastroenterol Nutr 25:371-376.
Garcia-Zepeda E., Rothenberg M.E., Ownbey R.T., Celestin J., Leder P., Luster A.D. (1996) Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat Med 2:449-456.
Best W.R., Bektel J.M., Singleton J.W., Kern F. (1976) Development of a Crohn's disease activity index: National cooperative Crohn's disease study. Gastroenterology 70:439-444.
Hansel T.T., De Vries I.J.M., Iff T., Rihs S., Wanolzilak M., Betz S. (1991) An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J Immunol Methods 145:105-110.
Boyden S. (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J Exp Med 115:453-466.
Louis R., Shute J., Biagi S., Stanciu L., Marrell F., Temor H. (1997) Cell infiltration, ICAM-1 expression, and eosinophil chemotactic activity in asthmatic sputum. Am J Respir Crit Care Med 155:466-472.
Warringa R.A.J., Mengelers H.J.J., Kuijper P.H.M., Raaijmakers J.A.M., Bruijnzeel P.L.B., Koenderman L. (1992) In vivo priming of platelet-activating factor-induced eosinophil chemotaxis in allergic asthmatic individuals. Blood 79:1836-1841.
Lampinen M., Rak S., Venge P. (1999) The role of interleukin-5, interleukin-8 and RANTES in the chemotactic attraction of eosinophils to the allergic lung. Clin Exp Allergy 29:314-322.
Schweizer R.C., Welmers B.A.C., Raaijmakers J.A.M., Zanen P., Lammers J.-W.J., Koenderman L. (1994) RANTES- and interleukin-8-induced responses in normal human eosinophils: Effects of priming with interleukin-5. Blood 83:3697-3704.
Petering H., Götze O., Kimmig D., Smolarski R., Kapp A., Elsner J. (1999) The biologic role of interleukin-8: Functional analysis and expression of CXCR1 and CXCR2 on human eosinophils. Blood 93:694-702.
Chuntharapai A., Kim J. (1995) Regulation of the expression of IL-8 receptor A/B by IL-8: Possible functions of each receptor. J Immunol 155:2587-2594.
Heath H., Qin S., Rao P., Wu L., LaRosa G., Kassam N. (1997) Chemokine receptor usage by human eosinophils. J Clin Invest 99:178-184.
Warringa R.A.J., Koenderman L., Kok P.T.M., Kreukniet J., Bruijnzeel P.L.B. (1991) Modulation and induction of eosinophil chemotaxis by granulocyte-macrophage colony-stimulating factor and interleukin-3. Blood 77:2694-2700.
Louis E., Belaiche J., Van Kemseke C., Franchimont D., De Groote D., Geenen V. (1997) A high serum concentration of interleukin-6 is predictive of relapse in quiescent Crohn's disease. Eur J Gastroenterol Hepatol 9:939-944.
Desreumaux P., Brandt E., Gambiez L., Emilie D., Geboes K., Klein O. (1997) Distinct cytokine patterns in early and chronic ileal lesions of Crohn's disease. Gastroenterology 113:118-126.
Lorentz A., Schwengberg S., Mierke C., Manns M.P., Bischoff S.C. (1999) Human intestinal mast cells produce IL-5 in vitro upon IgE receptor cross-linking and in vivo in the course of intestinal inflammatory disease. Eur J Immunol 29:1496-1503.
Gelbmann C.M., Mestermann S., Gross V., Kollinger M., Scholmerich J., Falk W. (1999) Strictures in Crohn's disease are characterized by an accumulation of mast cells colocalised with laminin but not with fibronectin or vitronectin. Gut 45:210-217.
Raithel M., Schneider H.T., Hahn E.G. (1999) Effect of substance P on histamine secretion from gut mucosa in inflammatory bowel disease. Scand J Gastroenterol 34:496-503.
Bettiol J., Radermecker M., Sele J., Henquet M., Cataldo D., Louis R. (1999) Airway mast-cell activation in asthmatics is associated with selective sputum eosinophilia. Allergy 54:1188-1193.
Brandt E., Muller-Alouf H., Desreumaux P., Woerly G., Colombel J.F., Capron M. (1998) Circulating growth-regulator oncogene alpha contributes to neutrophil priming and interleukin-8-directed mucosal recruitment into chronic lesions of patients with Crohn's disease. Eur Cytokine Netw 9:647-653.
Hällgren R., Colombel J.F., Dahl R., Kjell F., Kruse A., Jacobsen N.O. (1989) Neutrophil and eosinophil involvement of the small bowel in patients with celiac disease and Crohn's disease: Studies on the secretion rate and immunohistochemical localization of granulocyte granule constituents. Am J Med 86:56-63.
Liu H., Lazarus S.C., Caughey G.H., Fahy J.V. (1999) Neutrophil elastase and elastase-rich cystic fibrosis sputum degranulate human eosinophils in vitro. Am J Physiol (1 PART 1).
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