Article (Scientific journals)
Airway Mast-Cell Activation in Asthmatics Is Associated with Selective Sputum Eosinophilia
Bettiol, Jane; Radermecker, Maurice; Sele, Jocelyne et al.
1999In Allergy, 54 (11), p. 1188-93
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Keywords :
asthma; eosinophils; neutrophils; sputum
Abstract :
[en] BACKGROUND: Tryptase is a serine endoprotease selectively released from mast cells. Although mast cells are known to be activated after experimental allergic provocation, their role in naturally occurring asthma is still debated. METHODS: We have investigated the levels of tryptase in the whole induced sputum collected from 51 asthmatics (31 atopic and 20 intrinsic) seen in our outpatient clinic and 22 normal nonatopic healthy volunteers. Tryptase was measured by a new immunoassay based on B12 monoclonal antibody recognition of total tryptase (UniCAP System, Pharmacia) with a sensitivity of 1 ng/ml. RESULTS: While being below the threshold of detection in all normal volunteers, tryptase was detectable in the sputum from 9/51 asthmatics (18%) including five atopic and four intrinsic asthma cases. In these patients, among whom three were asymptomatic asthmatics, the values ranged between 1 and 6.1 ng/ml. The asthmatics with detectable sputum tryptase had greater sputum eosinophil counts (P<0.05) but lower neutrophil counts (P<0.05) than those in whom tryptase was undetectable. When compared to control subjects, asthmatics without tryptase had still greater eosinophil counts (P<0.0001) but also raised neutrophil counts (P<0.05). No significant difference could be found between asthmatics with tryptase and those without tryptase with respect to the age, the baseline lung function, the methacholine bronchial responsiveness, and the frequency of treatment with inhaled steroids. CONCLUSIONS: With the UniCAP System, tryptase was detectable in the sputum from 18% of asthmatics irrespective of atopy and current symptoms. Asthmatics with tryptase appeared to have a selective increase in sputum eosinophil counts while those without tryptase displayed a mixed sputum granulocyte infiltration with raised eosinophil and neutrophil counts.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Bettiol, Jane
Radermecker, Maurice ;  Université de Liège - ULiège > Pneumologie-Allergologie
Sele, Jocelyne ;  Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
Henquet, Monique
Cataldo, Didier  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement - Département des sciences biomédicales et précliniques
Louis, Renaud ;  Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Language :
English
Title :
Airway Mast-Cell Activation in Asthmatics Is Associated with Selective Sputum Eosinophilia
Publication date :
November 1999
Journal title :
Allergy
ISSN :
0105-4538
eISSN :
1398-9995
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
54
Issue :
11
Pages :
1188-93
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 November 2009

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