Phytotoxicity and Plant Defence Induction by Cinnamomum cassia Essential Oil Application on Malus domestica Tree: A Molecular Approach

Werrie, Pierre-Yves; Juillard, Anthony; Heintz, Christelle; Brisset, Marie-Noëlle; Fauconnier, Marie-Laure

doi:10.3390/agronomy12020512

Article (Scientific journals)

Phytotoxicity and Plant Defence Induction by Cinnamomum cassia Essential Oil Application on Malus domestica Tree: A Molecular Approach

Werrie, Pierre-Yves; Juillard, Anthony; Heintz, Christelle et al.

2022 • In Agronomy, 12 (2), p. 512

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https://hdl.handle.net/2268/268693

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10.3390/agronomy12020512

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Keywords :

Cinnamomum cassia; biopesticides; essential oil; molecular mechanism; oxidative burst; defence induction; Agronomy and Crop Science; phytotoxicity

Abstract :

[en] Essential oils (EOs) are actively investigated as an alternative to numerous synthetic biocide products. Due to their large spectra of biological activities, the impact of EOs on non-target organisms should be characterized for biopesticide development purposes. In this study the potential phytotoxicity of Cinnamomum cassia EO (CEO) on apple trees (Malus domestica) was investigated in terms of oxidative burst (glutathione redox state) and damage (malondialdehyde). At 2%, CEO concentration the reduced glutathione leaf content drops from 269.6 ± 45.8 to 143 ± 28.4 nmol g−1 FW, after 30 min, illustrating a rapid and strong oxidative burst. Regarding oxidative damage, malondialdehyde increased significantly 24 h post application to 10.7 ± 3.05 nmol g−1 FW. Plant defence induction was previously suspected after trans-cinnamaldehyde (CEO main compound) application. Therefore, the elicitor potential was investigated by qRT-PCR, on the expression level of 29 genes related to major defence pathways (PR protein, secondary metabolism, oxidative stress, parietal modification). Multivariate analysis and increased expression levels suggest induction of systemic resistance. Hence, the present research illustrates the dose–dependent phytotoxicity of CEO in terms of lipid peroxidation. Transcriptional data illustrates the elicitor properties of CEO. These findings can help to design pest management strategies considering both their risks (phytotoxicity) and benefits (defence activation combined with direct biocide properties).

Disciplines :

Agriculture & agronomy
Anatomy (cytology, histology, embryology...) & physiology
Phytobiology (plant sciences, forestry, mycology...)
Chemistry

Author, co-author :

Werrie, Pierre-Yves ; Université de Liège - ULiège > TERRA Research Centre

Juillard, Anthony; University Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France

Heintz, Christelle; University Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France

Brisset, Marie-Noëlle; University Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France

Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT

Language :

English

Title :

Phytotoxicity and Plant Defence Induction by Cinnamomum cassia Essential Oil Application on Malus domestica Tree: A Molecular Approach

Publication date :

18 February 2022

Journal title :

Agronomy

eISSN :

2073-4395

Publisher :

MDPI AG, Basel, Switzerland

Volume :

Issue :

Pages :

512

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.mdpi.com/2073-4395/12/2/512/pdf

Funders :

Department of Research and Technological Development of the Walloon regions of Belgium

Available on ORBi :

since 07 March 2022

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