[en] Three sampling procedures were compared to determine the optimal technique for isolating mycoplasmas in cattle with respiratory diseases. The prevalence of mycoplasmas isolated from these animals is also reported. In the first group, bronchoalveolar lavage (BAL) and nasal swab cultures were compared with the corresponding lung cultures from cattle necropsied for fatal respiratory diseases (n = 20). In a second group, nasal swabs were compared with corresponding BAL cultures in living animals with recurrent respiratory pathologies (n = 49). There was complete agreement between the paired BAL and lung cultures. In contrast, nasal cultures were not representative of the mycoplasmas present in the lower respiratory airways. The relative sensitivity and specificity of the nasal swab technique compared to BAL in living animals confirmed that the nasal swab cultures were not predictive of lower respiratory airway pathogens, such as Mycoplasma bovis. BAL is considered to be the best method for isolating M. bovis in cattle with respiratory diseases as it combines reliability and feasibility under field sampling conditions. In the present study, Mycoplasma dispar (43%) and M. bovis (29%) were mainly isolated in mixed infections. This confirms the need to search for mycoplasmas in routine examinations and to take them into account in therapeutic strategies for respiratory diseases in cattle.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Thomas, Anne
Dizier, Isabelle ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Trolin, A.
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Linden, Annick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage
Language :
English
Title :
Comparison of sampling procedures for isolating pulmonary mycoplasmas in cattle
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Adegboye D.S., Halbur P.G., Nutsch R.G., Kadlec R.G., Rosenbusch R.F. (1996) Mycoplasma bovis-associated pneumonia and arthritis complicated with pyogranulomatous tenosynovitis in calves. Journal of American Veterinary Medicine Association 109:647-649.
Allen J.W., Viel L., Bateman K.G., Rosendal S., Shewen P.E., Physick-Sheard P. (1991) The microbial flora of the respiratory tract in feedlot calves: Associations between nasopharyngeal and BAL cultures. Journal of Veterinary Research 55:341-346.
Almeida R., Rosenbusch R. (1994) Impaired tracheoclearance of bacteria in calves infected with Mycoplasma dispar. Journal of Veterinary Medicine B 41:473-482.
Armstrong D.S., Grimwood K., Carlin J.B., Carzino R., Olinsky A., Phelan P.D. (1996) BAL and oropharyngeal cultures to identify lower respiratory pathogens in infants with cystic fibrosis. Pediatric Pulmonology 21:267-275.
Ball H.J., Finlay D., Reilly G.A.C. (1994) Sandwich ELISA detection of Mycoplasma bovis in pneumonic calf lungs and nasal swabs. The Veterinary Record 135:531-532.
Gourlay R.N., Howard C.J. (1978) Isolation and pathogenicity of mycoplasmas from the respiratory tract of calves. Current Topics in Veterinary Medicine 3:295-304.
Gourlay R.N., Howard C.J., Thomas L.H., Wyld S.G. (1979) Pathogenicity of some Mycoplasma and Acholeplasma species in the lungs of gnotobiotic calves. Research in Veterinary Science 27:233-237.
Kusiluka L.J., Ojeniyi B., Friis N.F. (2000) Increasing prevalence of Mycoplasma bovine in Danish cattle. Acta Veterinaria Scandinavica 41:139-146.
Nicholas R., Baker S. (1998) Recovery of mycoplasmas from animals. Mycoplasma Protocols. Methods in Molecular Biology , R. Miles and R. Nicholas (eds), (Humana Press, Totowa, NJ); 104:37-44.
Poumarat F., Perrin B., Longchambon D. (1991) Identification of ruminant mycoplasmas by dot immunoblotting on membrane filtration (MF dot). Veterinary Microbiology 29:329-338.
Poumarat F., Le Grand D., Bergonier D. (1996) Propriétés générales des mycoplasmes et hypervariabilité antigénique. Le Point Vétérinaire 28:761-769.
Reilly G.A., Ball H.J., Cassidy J.P., Bryson T.D. (1993) First reported isolation of Mycoplasma bovis from pneumonic calves in Northern Ireland. The Veterinary Record 133:550-551.
Schreiber P., Thomas A., Linden A., Mainil J., Collard A. (1999) Bactériologie comparée des liquides de lavage pulmonaire obtenus par voie transtrachéale ou par voie nasotrachéale chez le veau. Annales de Médecine Vétérinaire 144:169-174.
Springer W.T., Fulton R.W., Hagstad H.V., Nicholson S.S., Garton J.D. (1982) Prevalence of Mycoplasma and Chlamydia in the nasal flora of dairy calves. Veterinary Microbiology 7:351-357.
ter Laak E.A., Noordergraaf J.H., Dieltjes R.P.J.W. (1992) Prevalence of mycoplasmas in the respiratory tracts of pneumonic calves. Journal of Veterinary Medicine B 39:553-562.
ter Laak E.A., Noordergraaf J.H., Boomsluiter E. (1992) The nasal flora of healthy calves and cows. Journal of Veterinary Medicine B 39:610-616.
Thomas L.H., Howard C.J. (1974) Effect of Mycoplasma dispar, Mycoplasma bovirhinis, Acholeplasma laidlawii and T-mycoplasmas on explant cultures of bovine trachea. Journal of Comparative Pathology 84:193-202.
Thomas A., Dizier I., Trolin A., Lacroix N., Schreiber P., Mainil J., Linden A. (1999) Microbiological examinations of BAL fluid in calves with relapsing bouts of acute respiratory symptoms suggest a central role for Mycoplasma bovis and/or Mycoplasma dispar., Proceedings of the 17th International Symposium. Comparative Respiratory Society, Harrison Hot Springs; 103.
Thomas A., Dizier I., Linden A., Mainil J., Bell C., Ball H. (2000) Application of an antigen-capture ELISA for the detection of Mycoplasma dispar in BAL fluids from Belgian cattle., Proceedings of the 13th International Congress of the International Organization of Mycoplasmology, Fukuoka; 259.
Thomas A., Nicolas C., Dizier I., Mainil J., Linden A. (2001) Antibiotic resistance of Belgian Mycoplasma bovis strains., Proceedings of a Conference on the Advances in Animal and Human Mycoplasmology, Weybridge, UK; 32-36.
Wang D., Clement P., Lauwers S. (1994) Comparison of bacterial culture results in BAL and nasal lavage fluid in children with pulmonary infection. International Journal of Pediatric Otorhinolaryngology 28:149-155.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.