[en] Newborn colostrum-restricted calves were orally inoculated with an Escherichia coli strain, identified originally as non-pathogenic, and into which the plasmid pVir was conjugally transferred. This resulted in diarrhea, intestinal lesions and extra-intestinal invasion, suggesting that factors affecting these pathogenic properties are located on pVir. In order to analyze the respective roles of the toxins CNF2 and CDTIII in the pathogenesis, colostrum-restricted calves were inoculated with isogenic mutants in the cnf2 and the cdt-III genes. The loss of cnf2 is associated with a reduction in the pathogenicity, since diarrhea does not occur in calves challenged, in spite of successful colonization of the intestine. Nevertheless, the mutant strain remains able to invade the bloodstream and to localize in the internal organs. Conversely, the calves inoculated with mutant in the cdt-III gene evolved in the same way as wild-type strain-inoculated calves with regard to clinical signs and macroscopic or microscopic lesions. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
Disciplines :
Immunology & infectious disease Microbiology
Author, co-author :
Van Bost, S.
Roels, S.
Oswald, E.
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Putative roles of the CNF2 and CDTIII toxins in experimental infections with necrotoxigenic Escherichia coli type 2 (NTEC2) strains in calves
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Blanco M., Blanco J., Blanco J.E., Ramos J. Enterotoxigenic, verotoxigenic, and necrotoxigenic Escherichia coli isolated from cattle in Spain. Am. J. Vet. Res. 54:1993;1446-1451.
Caprioli A., Falbo V., Roda L.G., Ruggeri F.M., Zona C. Partial purification and characterization of an Escherichia coli toxic factor that induces morphological cell alterations. Infect. Immun. 39:1983;1300-1306.
De Rycke J., Gonzales E.A., Blanco J., Oswald E., Blanco M., Boivin R. Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. J. Clin. Microbiol. 28:1990;694-699.
De Rycke J., Guillot J.F., Boivin R. Cytotoxins in non-enterotoxigenic strains of Escherichia coli isolated from faeces of diarrheic calves. Vet. Microbiol. 15:1987;137-150.
Falbo V., Famiglietti M., Caprioli A. Gene block encoding production of cytotoxic necrotizing factor 1 and hemolysin in Escherichia coli isolates from extraintestinal infections. Infect. Immun. 60:1992;2182-2187.
Oswald E., De Rycke J., Guillot J.F., Boivin R. Cytotoxic effect of multinucleation in HeLa cell cultures associated with the presence of Vir plasmid in Escherichia coli strains. FEMS Microbiol. Lett. 58:1989;95-100.
Smith H.W. A search for transmissible pathogenic characters in invasive strains of Escherichia coli: the discovery of a plasmid-controlled lethal character closely associated, or identified with colicin V. J. Gen. Microbiol. 83:1974;95-111.
Mainil J., Gerardin J., Jacquemin E. Identification of the F17 fimbrial subunit- and adhesin-encoding (f17A and f17G) gene variants in necrotoxigenic Escherichia coli from cattle, pigs and humans. Vet. Microbiol. 73:2000;327-335.
Gerardin J., Lalioui L., Jacquemin E., Le Bouguénec C., Mainil J. The afa related gene cluster in necrotoxigenic and other Escherichia coli from animals belongs to the afa-8 variant. Vet. Microbiol. 76:2000;175-184.
Pérès S.Y., Marchés O., Daigle F., Nougareyde J.-P., Herault F., Tasca C., De Rycke J., Oswald E. A new cytolethal distending toxin (CDT) from Escherichia coli producing CNF2 blocks HeLa cell division in G2/M phase. Mol. Microbiol. 24:1997;1095-1107.
Oswald E., Sugai M., Labigne A., Wu H.C., Fiorentini C., Boquet P., O'Brien A.D. Cytotoxic necrotizing factor type-2 produced by virulent Escherichia coli modifies the small GTP binding proteins Rho involved in assembly of actin stress fibers. Proc. Natl. Acad. Sci. USA. 91:1994;3814-3818.
Comayras C., Tascas C., Pérès S.Y., Ducommun B., Oswald E., De Rycke J. Escherichia coli cytolethal distending toxin blocks the HeLa cell cycle at G2/M transition by preventing cdc2 protein kinase dephosphorylation and activation. Infect. Immun. 65:1997;5088-5095.
Elliott S.J., Srinivas S., John Albert M., Alam K., Robins-Browne R.M., Gunzburg S.T., Mee B.J., Chang B.J. Characterization of the roles of hemolysin and other toxins in enteropathy caused by alpha-hemolytic Escherichia coli linked to human diarrhea. Infect. Immun. 66:1998;2040-2051.
Fournout S., Dozois C.M., Odin M., Desautels C., Pérès S.Y., Hérault F., Daigle F., Segafredo C., Laffitte J., Oswald E., Fairbrother J.M., Oswald I.P. Lack of a role of cytotoxic necrotizing factor 1 toxin from Escherichia coli in bacterial pathogenicity and host cytokine response in infected germfree piglets. Infect. Immun. 68:2000;839-847.
Van Bost S., Roels S., Mainil J. Necrotoxigenic Escherichia coli type-2 invade and cause diarrhoea during experimental infection in colostrum- restricted newborn calves. Vet. Microbiol. 81:2001;315-329.
Oswald E., De Rycke J., Lintermans P., Van Muylen K., Mainil J., Daube G., Pohl P. Virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of Escherichia coli. J. Clin. Microbiol. 29:1991;2522-2527.
second ed Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning: a laboratory manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Chandler M., Clerget M., Galas D.J. The transposition frequency of IS 1-flanked transposons is a function of their size. J. Mol. Biol. 154:1982;229-243.
Oswald E., Pohl P., Jacquemins E., Lintermans P., Van Muylens K., O'Brien A.D., Mainil J. Specific DNA probes to detect Escherichia coli strains producing cytotoxic necrotizing factor type-1 or type-2. J. Med. Microbiol. 40:1994;428-434.
Van Bost S., Bâbe M-H., Jacquemin E., Mainil J. Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970. Vet. Microbiol. 82:2001;311-320.
Feldman D.B., Gupta B.N. Histopathologic changes in laboratory animals resulting from various methods of euthanasia. Lab. Anim. Sci. 26:1976;218-222.
Lara-Tajero M., Galan J.E. A bacterial toxin that controls cells cycle progression as a deoxyribonuclease I-like protein. Science. 290:2000;354-357.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
