Article (Scientific journals)
Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures
Rusu, Dorina; Loret, S.; Peulen, Olivier et al.
2005In BMC Cell Biology, 6, p. 42-53
Peer Reviewed verified by ORBi
 

Files


Full Text
2005-Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures.pdf
Publisher postprint (1.53 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs, interactions of enteropathogenes bacteria strains with intestinal epithelium and other physiologic or pathologic phenomenon involving the digestive tract. Results: Cultures of bovine colonocytes and jejunocytes were obtained from organoid-enriched preparations, using a combination of enzymatic and mechanical disruption of the intestine epithelium, followed by an isopicnic centrifugation discarding most single cells. Confluent cell monolayers arising from plated organoids exhibited epithelium typical features, such as the pavement-like structure, the presence of apical microvilli and tight junctions. Accordingly, cells expressed several markers of enterocyte brush border (i.e. maltase, alkaline phosphatase and fatty acid binding protein) as well as an epithelial cytoskeleton component (cytokeratin 18). However, enterocyte primocultures were also positive for the vimentin immunostaining (mesenchyme marker). Vimentin expression studies showed that this gene is constitutively expressed in bovine enterocytes. Comparison of the vimentin expression profile with the pattern of brush border enzymes activities, suggested that the decrease of cell differentiation level observed during the enterocyte isolation procedure and early passages of the primoculture could result from a post-transcriptional de-repression of vimentin synthesis. The low differentiation level of bovine enterocytes in vitro could partly be counteracted adding butyrate (1-2 mM) or using a glucose-deprived culture medium. Conclusion: The present study describes several complementary approaches to characterize bovine primary cultures of intestinal cells. Cultured cells kept their morphologic and functional characteristics during several generations.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Rusu, Dorina 
Loret, S.
Peulen, Olivier  ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine)
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Dandrifosse, Guy ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures
Publication date :
01 December 2005
Journal title :
BMC Cell Biology
eISSN :
1471-2121
Publisher :
Biomed Central Ltd, London, United Kingdom
Volume :
6
Pages :
42-53
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 October 2009

Statistics


Number of views
72 (5 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
53
Scopus citations®
without self-citations
51
OpenCitations
 
50

Bibliography


Similar publications



Contact ORBi