Reference : Pituicyte stellation is prevented by RhoA-or Cdc42-dependent actin polymerization.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/26813
Pituicyte stellation is prevented by RhoA-or Cdc42-dependent actin polymerization.
English
Rosso, Lia [Université de Lausanne, Lausanne - Switzerland > > >Centre Intégratif de Génomique > > >]
Pierson, Patricia M [>Université de Nice-Sophia Antipolis, > > >Faculté des Sciences > Laboratoire de Physiologie Cellulaire et Moléculaire > >]
Golfier, Claire [>Université de Nice-Sophia Antipolis, > > >Faculté des Sciences > Laboratoire de Physiologie Cellulaire et Moléculaire > > > > > >]
Peteri-Brunback, Brigitta [>Université de Nice-Sophia Antipolis, > > >Faculté des Sciences > Laboratoire de Physiologie Cellulaire et Moléculaire > > > > > >]
Deroanne, Christophe mailto [Université de Liège - Université de Nice Sophia Antipolis > Département des sciences biomédicales et précliniques - Centre Antoine Lacassagne > Laboratoire de biologie des tissus conjonctifs - CNRS UMR 6543 > > > >]
Van Obberghen-Schilling, Ellen [>Université de Nice-Sophia Antipolis, > > >Faculté des Sciences > Laboratoire de Physiologie Cellulaire et Moléculaire > > > > > >]
Mienville, Jean-Marc [>Université de Nice-Sophia Antipolis, > > >Faculté des Sciences > Laboratoire de Physiologie Cellulaire et Moléculaire > > > > > >]
2007
Cellular and Molecular Neurobiology
Kluwer Academic/Plenum Publishers
27
6
791-804
Yes (verified by ORBi)
International
0272-4340
New York
NY
[en] Actins/metabolism ; Adenosine/pharmacology ; Animals ; Astrocytes/cytology/drug effects/metabolism ; Cells, Cultured ; Cytoskeleton/drug effects/metabolism ; Dimerization ; Neuronal Plasticity/physiology ; Pituitary Gland/cytology ; RNA Interference ; Rats ; Rats, Wistar ; Transfection ; cdc42 GTP-Binding Protein/genetics/metabolism/physiology ; rhoA GTP-Binding Protein/genetics/metabolism/physiology
[en] Our aim was to shed light on different steps leading from metabotropic receptor activation to changes in cell shape, such as those that characterize the morphological plasticity of neurohypophysial astrocytes (pituicytes). Using explant cultures of adult rat pituicytes, we have previously established that adenosine A1 receptor activation induces stellation via inhibition of RhoA monomeric GTPase and subsequent disruption of actin stress fibers. Here, we rule out RhoA phosphorylation as a mechanism for that inhibition. Rather, our results are more consistent with involvement of a GTPase-activating protein (GAP). siRNA and pull-down experiments suggest that a step downstream of RhoA might involve Cdc42, another GTPase of the Rho family. However, RhoA activation, e.g., in the presence of serum, induces stress fibers, whereas direct Cdc42 activation appears to confine actin within a submembrane - i.e., cortical - network, which also prevents stellation. Therefore, we propose that RhoA may activate Cdc42 in parallel with an effector, such as p160Rho-kinase, that induces and maintains actin stress fibers in a dominant fashion. Rac1 is not involved in the stellation process per se but appears to induce a dendritogenic effect. Ultimately, it may be stated that pituicyte stellation is inducible upon mere actin depolymerization, and preventable upon actin organization, be it in the form of stress fibers or in a cortical configuration.
Researchers
http://hdl.handle.net/2268/26813
10.1007/s10571-007-9176-7
The original publication is available at www.springerlink.com

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