P(3HB); P(3HB-co-3HV); Poly(3-hydroxybutyrate); Poly(3-hydroxybutyrate-co-3-hydroxyvalerate; emulsion templating; porous scaffold; salt leaching
Abstract :
[en] Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (>170% swelling in water) but fragile, probably due to the increase of the polymer's polydispersity index and its high porosity (>50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27-49% porosity) and hydrophilicity (>30% water swelling) and without impairing their mechanical properties (3.18-3.35 MPa tensile strength and 0.07-0.11 MPa Young's Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.
Research Center/Unit :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Materials science & engineering
Author, co-author :
Esmail, Asiyah
Pereira, João R.
Sevrin, Chantal
Grandfils, Christian ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Menda, Ugur Deneb
Fortunato, Elvira
Oliva, Abel
Freitas, Filomena
Language :
English
Title :
Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate).
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Anjum, A.; Zuber, M.; Zia, K.M.; Noreen, A.; Anjum, M.N.; Tabasum, S. Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements. Int. J. Biol. Macromol. 2016, 89, 161–174. [CrossRef]
Rathbone, S.; Furrer, P.; Lübben, J.; Zinn, M.; Cartmell, S. Biocompatibility of polyhydroxyalkanoate as a potential material for ligament and tendon scaffold material. J. Biomed. Mater. Res. Part A 2010, 93, 1391–1403. [CrossRef] [PubMed]
Knight, E.; Przyborski, S. Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro. J. Anat. 2015, 227, 746–756. [CrossRef]
Nikolova, M.P.; Chavali, M.S. Recent advances in biomaterials for 3D scaffolds: A review. Bioact. Mater. 2019, 4, 271–292. [CrossRef] [PubMed]
Dolcimascolo, A.; Calabrese, G.; Conoci, S.; Parenti, R. Innovative Biomaterials for Tissue Engineering. In Biomaterial-Supported Tissue Reconstruction or Regeneration; IntechOpen: London, UK, 2019; pp. 1–18. Available online: Https://www.intechopen.com/chapters/65513 (accessed on 1 August 2021).
Cameron, N.R. High internal phase emulsion templating as a route to well-defined porous polymers. Polymer 2005, 46, 1439–1449. [CrossRef]
Zubairi, S.I.; Bismarck, A.; Mantalaris, A. The effect of surface heterogeneity on wettability of porous three dimensional (3-D) scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV). J. Teknol. 2015, 75, 305–312. [CrossRef]
Cruz, M.V.; Sarraguça, M.C.; Freitas, F.; Lopes, J.A.; Reis, M.A.M. Online monitoring of P(3HB) produced from used cooking oil with near-infrared spectroscopy. J. Biotechnol. 2015, 194, 1–9. [CrossRef] [PubMed]
Wang, Y.; Chen, R.; Cai, J.Y.; Liu, Z.; Zheng, Y.; Wang, H.; Li, Q.; He, N. Biosynthesis and Thermal Properties of PHBV Produced from Levulinic Acid by Ralstonia eutropha. PLoS ONE 2013, 8, e60318. [CrossRef]
de Meneses, L.; Pereira, J.R.; Sevrin, C.; Grandfils, C.; Paiva, A.; Reis, M.A.M.; Freitas, F. Pseudomonas chlororaphis as a multiproduct platform: Conversion of glycerol into high-value biopolymers and phenazines. New Biotechnol. 2020, 55, 84–90. [CrossRef] [PubMed]
Rebocho, A.T.; Pereira, J.R.; Freitas, F.; Neves, L.A.; Alves, V.D.; Sevrin, C.; Grandfils, C.; Reis, M.A.M. Production of mediumchain length polyhydroxyalkanoates by Pseudomonas citronellolis grown in apple pulp waste. Appl. Food Biotechnol. 2019, 6, 71–82. [CrossRef]
Morais, C.; Freitas, F.; Cruz, M.V.; Paiva, A.; Dionísio, M.; Reis, M.A.M. Conversion of fat-containing waste from the margarine manufacturing process into bacterial polyhydroxyalkanoates. Int. J. Biol. Macromol. 2014, 71, 68–73. [CrossRef]
Kumar, P.T.S.; Lakshmanan, V.K.; Biswas, R.; Nair, S.V.; Jayakumar, R. Synthesis and biological evaluation of chitin hydrogel/nano ZnO composite bandage as antibacterial wound dressing. J. Biomed. Nanotechnol. 2012, 8, 891–900. [CrossRef]
Pereira, J.R.; Araújo, D.; Marques, A.C.; Neves, L.A.; Grandfils, C.; Sevrin, C.; Alves, V.D.; Fortunato, E.; Reis, M.A.M.; Freitas, F. Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol. Int. J. Biol. Macromol. 2018, 122, 1144–1151. [CrossRef]
El-Hadi, A.; Schnabel, R.; Straube, E.; Müller, G.; Henning, S. Correlation between degree of crystallinity, morphology, glass temperature, mechanical properties and biodegradation of Poly(3-hydroxyalkanoate) PHAs and their blends. Polym. Test. 2002, 21, 665–674. [CrossRef]
Bergstrand, A.; Uppström, S.; Larsson, A. Permeability of porous poly(3-hydroxybutyrate) barriers of single and bilayer type for implant applications. Int. J. Polym. Sci. 2014, 2014. [CrossRef]
Bergstrand, A.; Andersson, H.; Cramby, J.; Sott, K.; Larsson, A. Preparation of Porous Poly(3-Hydroxybutyrate) Films by Water-Droplet Templating. J. Biomater. Nanobiotechnol. 2012, 3, 431–439. [CrossRef]
Degeratu, C.N.; Zaharia, C.; Tudora, M.R.; Tucureanu, C.; Hubca, G. Influence of Porosity Upon Cells Adhesion on Polyhydroxyalkanoates Films. Chem. Bull. Politeh. Univ. Timis. 2010, 55, 189–192.
Zhang, D.; Cui, F.; Luo, Z.; Lin, Y.; Zhao, K.; Chen, G. Wettability improvement of bacterial polyhydroxyalkanoates via ion implantation. Surf. Coat. Technol. 2000. [CrossRef]
Ruiz, I.; Hermida, É.B.; Baldessari, A. Fabrication and characterization of porous PHBV scaffolds for tissue engineering. J. Phys. Conf. Ser. 2011, 332. [CrossRef]
Zhao, K.; Deng, Y.; Chen, J.C.; Chen, G.Q. Polyhydroxyalkanoate (PHA) scaffolds with good mechanical properties and biocompatibility. Biomaterials 2003, 24, 1041–1045. [CrossRef]
Zhu, X.; Zhong, T.; Huang, R.; Wan, A. Preparation of hydrophilic poly(lactic acid) tissue engineering scaffold via (PLA)-(PLAb- PEG)-(PEG) solution casting and thermal-induced surface structural transformation. J. Biomater. Sci. Polym. Ed. 2015, 26, 1286–1296. [CrossRef] [PubMed]
Modaress, M.P.; Mirzadeh, H.; Zandi, M. Fabrication of a porous wall and higher interconnectivity scaffold comprising gelatin/chitosan via combination of salt-leaching and lyophilization methods. Iran. Polym. J. English Ed. 2012, 21, 191–200. [CrossRef]
Reignier, J.; Huneault, M.A. Preparation of interconnected poly("{lunate}-caprolactone) porous scaffolds by a combination of polymer and salt particulate leaching. Polymer 2006, 47, 4703–4717. [CrossRef]
Oh, S.H.; Park, I.K.; Kim, J.M.; Lee, J.H. In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method. Biomaterials 2007, 28, 1664–1671. [CrossRef] [PubMed]
Torun Köse, G.; Kenar, H.; Hasirci, N.; Hasirci, V. Macroporous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) matrices for bone tissue engineering. Biomaterials 2003, 24, 1949–1958. [CrossRef]
Choudhury, M.; Mohanty, S.; Nayak, S. Effect of different solvents in solvent casting of porous PLA scaffolds—In biomedical and tissue engineering applications. J. Biomater. Tissue Eng. 2015, 5, 1–9. [CrossRef]
Pamua, E.; Ba, M.; Czajkowska, B.; Dobrzyñski, P.; Bero, M.; Kasperczyk, J. Elaboration and Characterization of Biodegradable Scaffolds from Poly with Low-Toxic Zirconium Acetylacetonate Abstract. Ann. Transplant. 2004, 9, 64–67.
Price, G.J.; West, P.J.; Smith, P.F. Control of polymer structure using power ultrasound. Ultrason.-Sonochem. 1994, 1, 51–57. [CrossRef]
Amaro, L.; Correia, D.M.; Martins, P.M.; Botelho, G.; Carabineiro, S.A.C.; Ribeiro, C.; Lanceros-Mendez, S. Morphology Dependence Degradation of Electro- and Magnetoactive Poly(3-hydroxybutyrate-co-hydroxyvalerate) for Tissue Engineering Applications. Polymer 2020, 12, 953. [CrossRef] [PubMed]
Jasso-Gastinel, C.F.; Soltero-Martínez, J.F.A.; Mendizábal, E. Introduction: Modifiable Characteristics and Applications. In Modification of Polymer Properties; William Andrew Publishing: New York, NY, USA, 2016; pp. 1–21. ISBN 9780323443982.
Conti, D.; Pezzin, H.; Anto, L.; Coelho, A. Mechanical and Morphological Properties of Poly(3-hydroxybutyrate)/Poly(3- hydroxybutyrate-co-3-hydroxyvalerate) Blends. Macromol. Symp. 2006, 1, 491–500. [CrossRef]
Lemechko, P.; Le Fellic, M.; Bruzaud, S. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) using agro-industrial effluents with tunable proportion of 3-hydroxyvalerate monomer units. Int. J. Biol. Macromol. 2019, 128, 429–434. [CrossRef]
Das, R.; Saha, N.R.; Pal, A.; Chattopadhyay, D.; Paul, A.K. Comparative evaluation of physico-chemical characteristics of biopolyesters P (3HB) and P (3HB-co-3HV) produced by endophytic Bacillus cereus RCL 02. Front. Biol. 2018, 13. [CrossRef]
Rebocho, A.T.; Pereira, J.R.; Neves, L.A.; Alves, V.D.; Sevrin, C.; Grandfils, C.; Freitas, F.; Reis, M.A.M. Preparation and characterization of films based on a natural p(3hb)/mcl-pha blend obtained through the co-culture of cupriavidus necator and pseudomonas citronellolis in apple pulp waste. Bioengineering 2020, 7, 34. [CrossRef] [PubMed]
Owen, R.; Sherborne, C.; Paterson, T.; Green, N.H.; Reilly, G.C.; Claeyssens, F. Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering. J. Mech. Behav. Biomed. Mater. 2016, 54, 159–172. [CrossRef] [PubMed]
Lim, X.; Potter, M.; Cui, Z.; Dye, J.F. Manufacture and characterisation of EmDerm—Novel hierarchically structured bio-active scaffolds for tissue regeneration. J. Mater. Sci. Mater. Med. 2018, 29. [CrossRef]
Naranda, J.; Sušec, M.; Maver, U.; Gradišnik, L.; Gorenjak, M.; Vukasovi´c, A.; Ivkovi´c, A.; Rupnik, M.S.; Vogrin, M.; Krajnc, P. Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration. Sci. Rep. 2016, 6, 28695. [CrossRef]
Wen, J.; Yao, J.; Chen, X.; Shao, Z. Silk Fibroin Acts as a Self-Emulsifier to Prepare Hierarchically Porous Silk Fibroin Scaffolds through Emulsion-Ice Dual Templates. ACS Omega 2018, 3, 3396–3405. [CrossRef] [PubMed]
Limpanuphap, S. Preparation of Porous Biomaterial Structures by a Solvent Casting/Particulate Leaching Technique. Asia-Pac. J. Sci. Technol. 2007, 12, 323–330.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
