[en] The lack of small-diameter vascular grafts (inner diameter <5 mm) to substitute autologous grafts in arterial bypass surgeries has a massive impact on the prognosis and progression of cardiovascular diseases, the leading cause of death globally. Decellularized arteries from different sources have been proposed as an alternative, but their poor mechanical performance and high collagen exposure, which promotes platelet and bacteria adhesion, limit their successful application. In this study, these limitations were surpassed for decellularized umbilical cord arteries through the coating of their lumen with graphene oxide (GO). Placental and umbilical cord arteries were decellularized and perfused with a suspension of GO (C/O ratio 2:1) with ∼1.5 μm lateral size. A homogeneous GO coating that completely covered the collagen fibers was obtained for both arteries, with improvement of mechanical properties being achieved for umbilical cord decellularized arteries. GO coating increased the maximum force in 27%, the burst pressure in 29%, the strain in 25%, and the compliance in 10%, compared to umbilical cord decellularized arteries. The achieved theoretical burst pressure (1960 mmHg) and compliance (13.9%/100 mmHg) are similar to the human saphenous vein and mammary artery, respectively, which are used nowadays as the gold standard in coronary and peripheral artery bypass surgeries. Furthermore, and very importantly, coatings with GO did not compromise the endothelial cell adhesion but decreased platelet and bacteria adhesion to decellularized arteries, which will impact on the prevention of thrombosis and infection, until full re-endothetialization is achieved. Overall, our results reveal that GO coating has an effective role in the improvement of decellularized umbilical cord artery performance, which is a huge step toward their application as a small-diameter vascular graft.
Disciplines :
Biotechnology
Author, co-author :
Pereira, A. T.
Schneider, K. H.
Henriques, P. C.
Grasl, C.
Ferreira Melo, Sofia ; Université de Liège - ULiège > GIGA Cardiovascular Sciences - Cardiology
Fernandes, I. P.
Kiss, H.
Martins, M. C. L.
Bergmeister, H.
Gonçalves, I. C.
Language :
English
Title :
Graphene Oxide Coating Improves the Mechanical and Biological Properties of Decellularized Umbilical Cord Arteries
Publication date :
21 July 2021
Journal title :
ACS Applied Materials and Interfaces
ISSN :
1944-8244
eISSN :
1944-8252
Publisher :
American Chemical Society, United States - District of Columbia
World Health Organization (WHO); Cardiovascular Diseases (CVDs); https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds); accessed 02/2021.
Caliskan, E.; de Souza, D. R.; Boning, A.; Liakopoulos, O. J.; Choi, Y. H.; Pepper, J.; Gibson, C. M.; Perrault, L. P.; Wolf, R. K.; Kim, K. B.; Emmert, M. Y. Saphenous Vein Grafts in Contemporary Coronary Artery Bypass Graft Surgery. Nat. Rev. Cardiol. 2020, 17, 155-169, 10.1038/s41569-019-0249-3
Hiob, M. A.; She, S.; Muiznieks, L. D.; Weiss, A. S. Biomaterials and Modifications in the Development of Small-Diameter Vascular Grafts. ACS Biomater. Sci. Eng. 2017, 3, 712-723, 10.1021/acsbiomaterials.6b00220
Pashneh-Tala, S.; MacNeil, S.; Claeyssens, F. The Tissue-Engineered Vascular Graft-Past, Present, and Future. Tissue Eng., Part B 2016, 22, 68-100, 10.1089/ten.teb.2015.0100
Rana, D.; Zreiqat, H.; Benkirane-Jessel, N.; Ramakrishna, S.; Ramalingam, M. Development of Decellularized Scaffolds for Stem Cell-Driven Tissue Engineering. J. Tissue Eng. Regener. Med. 2017, 11, 942-965, 10.1002/term.2061
Schneider, K. H.; Aigner, P.; Holnthoner, W.; Monforte, X.; Nurnberger, S.; Runzler, D.; Redl, H.; Teuschl, A. H. Decellularized Human Placenta Chorion Matrix as a Favorable Source of Small-Diameter Vascular Grafts. Acta Biomater. 2016, 29, 125-134, 10.1016/j.actbio.2015.09.038
Lin, C. H.; Hsia, K.; Ma, H.; Lee, H.; Lu, J. H. In Vivo Performance of Decellularized Vascular Grafts: A Review Article. Int. J. Mol. Sci. 2018, 19, 2101 10.3390/ijms19072101
Heine, J.; Schmiedl, A.; Cebotari, S.; Karck, M.; Mertsching, H.; Haverich, A.; Kallenbach, K. Tissue Engineering Human Small-Caliber Autologous Vessels Using a Xenogenous Decellularized Connective Tissue Matrix Approach: Preclinical Comparative Biomechanical Studies. Artif. Organs 2011, 35, 930-940, 10.1111/j.1525-1594.2010.01199.x
Tosun, Z.; McFetridge, P. S. Improved Recellularization of Ex Vivo Vascular Scaffolds Using Directed Transport Gradients to Modulate ECM Remodeling. Biotechnol. Bioeng. 2013, 110, 2035-2045, 10.1002/bit.24934
Tillman, B. W.; Yazdani, S. K.; Neff, L. P.; Corriere, M. A.; Christ, G. J.; Soker, S.; Atala, A.; Geary, R. L.; Yoo, J. J. Bioengineered Vascular Access Maintains Structural Integrity in Response to Arteriovenous Flow and Repeated Needle Puncture. J. Vasc. Surg. 2012, 56, 783-793, 10.1016/j.jvs.2012.02.030
Olausson, M.; Patil, P. B.; Kuna, V. K.; Chougule, P.; Hernandez, N.; Methe, K.; Kullberg-Lindh, C.; Borg, H.; Ejnell, H.; Sumitran-Holgersson, S. Transplantation of an Allogeneic Vein Bioengineered with Autologous Stem Cells: A Proof-of-Concept study. Lancet 2012, 380, 230-237, 10.1016/S0140-6736(12)60633-3
Qi, H.; Cheng, C.; Wang, X.; Yu, X. Preparation and Investigation of Novel SrCl2/DCMC-Modified (via DOPA) Decellularized Arteries with Excellent Physicochemical Properties and Cytocompatibility for Vascular Scaffolds. RSC Adv. 2018, 8, 30098-30105, 10.1039/C8RA06427J
Porzionato, A.; Stocco, E.; Barbon, S.; Grandi, F.; Macchi, V.; De Caro, R. Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives. Int. J. Mol. Sci. 2018, 19, 4117 10.3390/ijms19124117
Gu, Y.; Wang, F.; Wang, R.; Li, J.; Wang, C.; Li, L.; Xu, Z.; Zhang, J. Preparation and Evaluation of Decellularized Porcine Carotid Arteries Cross-Linked by Genipin: The Preliminary Results. Cell Tissue Banking 2018, 19, 311-321, 10.1007/s10561-017-9675-9
Schneider, K. H.; Rohringer, S.; Kapeller, B.; Grasl, C.; Kiss, H.; Heber, S.; Walter, I.; Teuschl, A. H.; Podesser, B. K.; Bergmeister, H. Riboflavin-Mediated Photooxidation to Improve the Characteristics of Decellularized Human Arterial Small Diameter Vascular Grafts. Acta Biomater. 2020, 116, 246-258, 10.1016/j.actbio.2020.08.037
López-Ruiz, E.; Venkateswaran, S.; Peran, M.; Jimenez, G.; Pernagallo, S.; Diaz-Mochon, J. J.; Tura-Ceide, O.; Arrebola, F.; Melchor, J.; Soto, J.; Rus, G.; Real, P. J.; Diaz-Ricart, M.; Conde-Gonzalez, A.; Bradley, M.; Marchal, J. A. Poly(ethylmethacrylate-co-diethylaminoethyl Acrylate) Coating Improves Endothelial Re-Population, Bio-Mechanical and Anti-Thrombogenic Properties of Decellularized Carotid Arteries for Blood Vessel Replacement. Sci. Rep. 2017, 7, 407 10.1038/s41598-017-00294-6
Iijima, M.; Aubin, H.; Steinbrink, M.; Schiffer, F.; Assmann, A.; Weisel, R. D.; Matsui, Y.; Li, R. K.; Lichtenberg, A.; Akhyari, P. Bioactive Coating of Decellularized Vascular Grafts with a Temperature-Sensitive VEGF-Conjugated Hydrogel Accelerates Autologous Endothelialization In Vivo. J. Tissue Eng. Regener. Med. 2018, 12, e513-e522, 10.1002/term.2321
Smith, R. J., Jr.; Koobatian, M. T.; Shahini, A.; Swartz, D. D.; Andreadis, S. T. Capture of Endothelial Cells Under Flow Using Immobilized Vascular Endothelial Growth Factor. Biomaterials 2015, 51, 303-312, 10.1016/j.biomaterials.2015.02.025
Lee, J. S.; Lee, K.; Moon, S. H.; Chung, H. M.; Lee, J. H.; Um, S. H.; Kim, D. I.; Cho, S. W. Mussel-Inspired Cell-Adhesion Peptide Modification for Enhanced Endothelialization of Decellularized Blood Vessels. Macromol. Biosci. 2014, 14, 1181-1189, 10.1002/mabi.201400052
Cai, Z.; Gu, Y.; Cheng, J.; Li, J.; Xu, Z.; Xing, Y.; Wang, C.; Wang, Z. Decellularization, Cross-Linking and Heparin Immobilization of Porcine Carotid Arteries for Tissue Engineering Vascular Grafts. Cell Tissue Banking 2019, 20, 569-578, 10.1007/s10561-019-09792-5
Zhou, X.; Zhao, B.; Wang, J.; Zhou, Y.; Chai, J.; Liu, H.; Zheng, Y.; Zhang, Y.; Xu, G.; Pan, H.; Zhang, H.; Zhou, J.; Liu, Y.; Zhen, M.; Zhao, Y. Engineered Vascular Graft Using Nanoscale Decellularized Arteries Modified with Controlled-Release Heparin and Vascular Endothelial Growth Factor. J. Biomater. Tissue Eng. 2016, 6, 870-882, 10.1166/jbt.2016.1516
Zhou, M.; Liu, Z.; Wei, Z.; Liu, C.; Qiao, T.; Ran, F.; Bai, Y.; Jiang, X.; Ding, Y. Development and Validation of Small-Diameter Vascular Tissue from a Decellularized Scaffold Coated with Heparin and Vascular Endothelial Growth Factor. Artif. Organs 2009, 33, 230-239, 10.1111/j.1525-1594.2009.00713.x
Schneider, K.; Enayati, M.; Grasl, C.; Walter, I.; Budinsky, L.; Zebic, G.; Kaun, C.; Wagner, A.; Kratochwill, K.; Redl, H.; Teuschl, A.; Podesser, B. K.; Bergmeister, H. Acellular Vascular Matrix Grafts from Human Placenta Chorion: Impact of ECM Preservation on Graft Characteristics, Protein Composition and In Vivo Performance. Biomaterials 2018, 177, 14-26, 10.1016/j.biomaterials.2018.05.045
Ilanlou, S.; Khakbiz, M.; Amoabediny, G.; Mohammadi, J.; Rabbani, H. Carboxymethyl Kappa Carrageenan-Modified Decellularized Small-Diameter Vascular Grafts Improving Thromboresistance Properties. J. Biomed. Mater. Res., Part A 2019, 107, 1690-1701, 10.1002/jbm.a.36684
Henriques, P. C.; Borges, I.; Pinto, A. M.; Magalhães, F. D.; Gonçalves, I. C. Fabrication and Antimicrobial Performance of Surfaces Integrating Graphene-Based Materials. Carbon 2018, 132, 709-732, 10.1016/j.carbon.2018.02.027
Gomes, R. N.; Borges, I.; Pereira, A. T.; Maia, A. F.; Pestana, M.; Magalhães, F. D.; Pinto, A. M.; Gonçalves, I. C. Antimicrobial Graphene Nanoplatelets Coatings for Silicone Catheters. Carbon 2018, 139, 635-647, 10.1016/j.carbon.2018.06.044
Pereira, A. T.; Henriques, P. C.; Costa, P. C.; Martins, M. C. L.; Magalhães, F. D.; Gonçalves, I. C. Graphene Oxide-Reinforced Poly(2-hydroxyethyl Methacrylate) Hydrogels with Extreme Stiffness and High-Strength. Compos. Sci. Technol. 2019, 184, 107819 10.1016/j.compscitech.2019.107819
Pereira, A. T.; Henriques, P. C.; Schneider, K. H.; Pires, A. L.; Pereira, A. M.; Martins, M. C. L.; Magalhaes, F. D.; Bergmeister, H.; Goncalves, I. C. Graphene-Based Materials: The Key for the Successful Application of pHEMA as a Blood-Contacting Device Dagger. Biomater. Sci. 2021, 9, 3362-3377, 10.1039/D0BM01699C
Papageorgiou, D. G.; Kinloch, I. A.; Young, R. J. Mechanical Properties of Graphene and Graphene-Based Nanocomposites. Prog. Mater. Sci. 2017, 90, 75-127, 10.1016/j.pmatsci.2017.07.004
Raza, M. A.; Mujddid, M.; Hussain, M.; Ali, H. Q.; Rehman, Z. U.; Inam, A. Mechanical Properties of Graphene Oxide Coated-Glass Fiber Reinforced Unsaturated Polyester Composites. Mater. Res. Express 2019, 6, 115303 10.1088/2053-1591/ab43a1
Mahmood, H.; Vanzetti, L.; Bersani, M.; Pegoretti, A. Mechanical Properties and Strain Monitoring of Glass-Epoxy Composites with Graphene-Coated Fibers. Composites, Part A 2018, 107, 112-123, 10.1016/j.compositesa.2017.12.023
Li, F.; Hua, Y.; Qu, C.-B.; Xiao, H.-M.; Fu, S.-Y. Greatly Enhanced Cryogenic Mechanical Properties of Short Carbon Fiber/Polyethersulfone Composites by Graphene Oxide Coating. Composites, Part A 2016, 89, 47-55, 10.1016/j.compositesa.2016.02.016
Bhanuprakash, L.; Parasuram, S.; Varghese, S. Experimental Investigation on Graphene Oxides Coated Carbon Fibre/Epoxy Hybrid Composites: Mechanical and Electrical Properties. Compos. Sci. Technol. 2019, 179, 134-144, 10.1016/j.compscitech.2019.04.034
Toker, M.; Rostami, S.; Kesici, M.; Gul, O.; Kocaturk, O.; Odabas, S.; Garipcan, B. Decellularization and Characterization of Leek: A Potential Cellulose-Based Biomaterial. Cellulose 2020, 27, 7331-7348, 10.1007/s10570-020-03278-4
Wang, Q.; Chen, J.; Niu, Q.; Fu, X.; Sun, X.; Tong, X. The Application of Graphene Oxidized Combining with Decellularized Scaffold to Repair of Sciatic Nerve Injury in Rats. Saudi Pharm. J. 2017, 25, 469-476, 10.1016/j.jsps.2017.04.008
Wilczek, P.; Major, R.; Lipinska, L.; Lackner, J.; Mzyk, A. Thrombogenicity and Biocompatibility Studies of Reduced Graphene Oxide Modified Acellular Pulmonary Valve Tissue. Mater. Sci. Eng. C 2015, 53, 310-321, 10.1016/j.msec.2015.04.044
Huo, D.; Liu, G.; Li, Y. Z.; Wang, Y. X.; Guan, G.; Yang, M. C.; Wei, K. Y.; Yang, J. Y.; Zeng, L. Q.; Li, G.; Zeng, W.; Zhu, C. H. Construction of Antithrombotic Tissue-Engineered Blood Vessel via Reduced Graphene Oxide Based Dual-Enzyme Biomimetic Cascade. ACS Nano 2017, 11, 10964-10973, 10.1021/acsnano.7b04836
Bimpong, S.; Abaidoo, C. S.; Atuahene, O. O.-D.; Tetteh, J. Morphometric Characterization of Umbilical Cord Vessels and Neonatal Outcome. Int. J. Anat. Res. 2019, 7, 6050-6058, 10.16965/ijar.2018.404
Gordon, Z.; Elad, D.; Almog, R.; Hazan, Y.; Jaffa, A. J.; Eytan, O. Anthropometry of Fetal Vasculature in the Chorionic Plate. J. Anat. 2007, 211, 698-706, 10.1111/j.1469-7580.2007.00819.x
Kovtun, A.; Jones, D.; Dell'Elce, S.; Treossi, E.; Liscio, A.; Palermo, V. Accurate Chemical Analysis of Oxygenated Graphene-Based Materials Using X-Ray Photoelectron Spectroscopy. Carbon 2019, 143, 268-275, 10.1016/j.carbon.2018.11.012
Bergmeister, H.; Schreiber, C.; Grasl, C.; Walter, I.; Plasenzotti, R.; Stoiber, M.; Bernhard, D.; Schima, H. Healing Characteristics of Electrospun Polyurethane Grafts with Various Porosities. Acta Biomater. 2013, 9, 6032-6040, 10.1016/j.actbio.2012.12.009
Stobinski, L.; Lesiak, B.; Malolepszy, A.; Mazurkiewicz, M.; Mierzwa, B.; Zemek, J.; Jiricek, P.; Bieloshapka, I. Graphene Oxide and Reduced Graphene Oxide Studied by the XRD, TEM and Electron Spectroscopy Methods. J. Electron Spectrosc. Relat. Phenom. 2014, 195, 145-154, 10.1016/j.elspec.2014.07.003
Lotya, M.; Rakovich, A.; Donegan, J. F.; Coleman, J. N. Measuring the Lateral Size of Liquid-Exfoliated Nanosheets with Dynamic Light Scattering. Nanotechnology 2013, 24, 265703 10.1088/0957-4484/24/26/265703
Catto, V.; Farè, S.; Freddi, G.; Tanzi, M. C. Vascular Tissue Engineering: Recent Advances in Small Diameter Blood Vessel Regeneration. ISRN Vasc. Med. 2014, 2014, 923030 10.1155/2014/923030
Lemson, M. S.; Tordoir, J. H.; Daemen, M. J.; Kitslaar, P. J. Intimal Hyperplasia in Vascular Grafts. Eur. J. Vasc. Endovasc. Surg. 2000, 19, 336-350, 10.1053/ejvs.1999.1040
Konig, G.; McAllister, T. N.; Dusserre, N.; Garrido, S. A.; Iyican, C.; Marini, A.; Fiorillo, A.; Avila, H.; Wystrychowski, W.; Zagalski, K.; Maruszewski, M.; Jones, A. L.; Cierpka, L.; de la Fuente, L. M.; L'Heureux, N. Mechanical Properties of Completely Autologous Human Tissue Engineered Blood Vessels Compared to Human Saphenous Vein and Mammary Artery. Biomaterials 2009, 30, 1542-1550, 10.1016/j.biomaterials.2008.11.011
Lamm, P.; Juchem, G.; Milz, S.; Schuffenhauer, M.; Reichart, B. Autologous Endothelialized Vein Allograft: A Solution in the Search for Small-Caliber Grafts in Coronary Artery Bypass Graft Operations. Circulation 2001, 104, I108-I114, 10.1161/hc37t1.094527
Norahan, M. H.; Amroon, M.; Ghahremanzadeh, R.; Mahmoodi, M.; Baheiraei, N. Electroactive Graphene Oxide-Incorporated Collagen Assisting Vascularization for Cardiac Tissue Engineering. J. Biomed. Mater. Res., Part A 2019, 107, 204-219, 10.1002/jbm.a.36555
Jing, X.; Mi, H.-Y.; Salick, M. R.; Cordie, T. M.; Peng, X.-F.; Turng, L.-S. Electrospinning Thermoplastic Polyurethane/Graphene Oxide Scaffolds for Small Diameter Vascular Graft Applications. Mater. Sci. Eng. C 2015, 49, 40-50, 10.1016/j.msec.2014.12.060
Pan, C. J.; Pang, L. Q.; Gao, F.; Wang, Y. N.; Liu, T.; Ye, W.; Hou, Y. H. Anticoagulation and Endothelial Cell Behaviors of Heparin-Loaded Graphene Oxide Coating on Titanium Surface. Mater. Sci. Eng. C 2016, 63, 333-340, 10.1016/j.msec.2016.03.001
Podila, R.; Moore, T.; Alexis, F.; Rao, A. M. Graphene Coatings for Enhanced Hemo-Compatibility of Nitinol Stents. RSC Adv. 2013, 3, 1660-1665, 10.1039/C2RA23073A
Mukherjee, S.; Sriram, P.; Barui, A. K.; Nethi, S. K.; Veeriah, V.; Chatterjee, S.; Suresh, K. I.; Patra, C. R. Graphene Oxides Show Angiogenic Properties. Adv. Healthcare Mater. 2015, 4, 1722-1732, 10.1002/adhm.201500155
Kenry; Lee, W. C.; Loh, K. P.; Lim, C. T. When Stem Cells Meet Graphene: Opportunities and Challenges in Regenerative Medicine. Biomaterials 2018, 155, 236-250, 10.1016/j.biomaterials.2017.10.004
Henriques, P. C.; Pereira, A. T.; Pires, A. L.; Pereira, A. M.; Magalhaes, F. D.; Goncalves, I. C. Graphene Surfaces Interaction with Bacteria, Mammalian Cells and Blood Constituents: The Impact of Graphene Platelets Oxidation and Thickness. ACS Appl. Mater. Interfaces 2020, 12, 21020-21035, 10.1021/acsami.9b21841
Thampi, S.; Nandkumar, A. M.; Muthuvijayan, V.; Parameswaran, R. Differential Adhesive and Bioactive Properties of the Polymeric Surface Coated with Graphene Oxide Thin Film. ACS Appl. Mater. Interfaces 2017, 9, 4498-4508, 10.1021/acsami.6b14863
Modi, A.; Verma, S. K.; Bellare, J. Graphene Oxide-Doping Improves the Biocompatibility and Separation Performance of Polyethersulfone Hollow Fiber Membranes for Bioartificial Kidney Application. J. Colloid Interface Sci. 2018, 514, 750-759, 10.1016/j.jcis.2017.12.044
Ma, L.; Huang, L.; Zhang, Y.; Zhao, L.; Xin, Q.; Ye, H.; Li, H. Hemocompatible Poly(lactic Acid) Membranes Prepared by Immobilizing Carboxylated Graphene Oxide via Mussel-Inspired Method for Hemodialysis. RSC Adv. 2018, 8, 153-161, 10.1039/C7RA11091J
Gharamti, A.; Kanafani, Z. A. Vascular Graft Infections: An Update. Infect. Dis. Clin. North Am. 2018, 32, 789-809, 10.1016/j.idc.2018.06.003
Holderbaum, D.; Hall, G. S.; Ehrhart, L. A. Collagen Binding to Staphylococcus aureus. Infect. Immun. 1986, 54, 359-364, 10.1128/iai.54.2.359-364.1986
Kuusela, P. Fibronectin Binds to Staphylococcus aureus. Nature 1978, 276, 718-720, 10.1038/276718a0
Park, P. W.; Roberts, D. D.; Grosso, L. E.; Parks, W. C.; Rosenbloom, J.; Abrams, W. R.; Mecham, R. P. Binding of Elastin to Staphylococcus aureus. J. Biol. Chem. 1991, 266, 23399-23406, 10.1016/S0021-9258(18)54510-5
Lopes, J. D.; dos Reis, M.; Brentani, R. R. Presence of Laminin Receptors in Staphylococcus aureus. Science 1985, 229, 275-277, 10.1126/science.3160113
Chhatwal, G. S.; Preissner, K. T.; Muller-Berghaus, G.; Blobel, H. Specific Binding of the Human S Protein (Vitronectin) to Streptococci, Staphylococcus aureus, and Escherichia coli. Infect. Immun. 1987, 55, 1878-1883, 10.1128/iai.55.8.1878-1883.1987
Hawiger, J.; Timmons, S.; Strong, D. D.; Cottrell, B. A.; Riley, M.; Doolittle, R. F. Identification of a Region of Human Fibrinogen Interacting with Staphylococcal Clumping Factor. Biochemistry 1982, 21, 1407-1413, 10.1021/bi00535a047
Yadav, N.; Dubey, A.; Shukla, S.; Saini, C. P.; Gupta, G.; Priyadarshini, R.; Lochab, B. Graphene Oxide-Coated Surface: Inhibition of Bacterial Biofilm Formation due to Specific Surface-Interface Interactions. ACS Omega 2017, 2, 3070-3082, 10.1021/acsomega.7b00371
Borges, I.; Henriques, P. C.; Gomes, R. N.; Pinto, A. M.; Pestana, M.; Magalhaes, F. D.; Goncalves, I. C. Exposure of Smaller and Oxidized Graphene on Polyurethane Surface Improves its Antimicrobial Performance. Nanomaterials 2020, 10, 349 10.3390/nano10020349
Melo, S. F.; Neves, S. C.; Pereira, A. T.; Borges, I.; Granja, P. L.; Magalhães, F. D.; Gonçalves, I. C. Incorporation of Graphene Oxide into Poly(ϵ-caprolactone) 3D Printed Fibrous Scaffolds Improves their Antimicrobial Properties. Mater. Sci. Eng. C 2020, 109, 110537 10.1016/j.msec.2019.110537