Article (Scientific journals)
Loss of apoptosis repressor with caspase recruitment domain (ARC) worsens high fat diet-induced hyperglycemia in mice.
Templin, Andrew T.; Schmidt, Christine; Hogan, Meghan F. et al.
2021In The Journal of endocrinology, 251 (2), p. 125-135
Peer reviewed
 

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Keywords :
Animals; Apoptosis Regulatory Proteins/physiology; Blood Glucose; Diet, High-Fat/adverse effects; Hyperglycemia/etiology; Insulin Secretion; Insulin-Secreting Cells/physiology; Mice; Muscle Proteins/physiology; ARC; cell death; diabetes; islet; β cell
Abstract :
[en] Apoptosis repressor with caspase recruitment domain (ARC) is an endogenous inhibitor of cell death signaling that is expressed in insulin-producing β cells. ARC has been shown to reduce β-cell death in response to diabetogenic stimuli in vitro, but its role in maintaining glucose homeostasis in vivo has not been fully established. Here we examined whether loss of ARC in FVB background mice exacerbates high fat diet (HFD)-induced hyperglycemia in vivo over 24 weeks. Prior to commencing 24-week HFD, ARC-/- mice had lower body weight than wild type (WT) mice. This body weight difference was maintained until the end of the study and was associated with decreased epididymal and inguinal adipose tissue mass in ARC-/- mice. Non-fasting plasma glucose was not different between ARC-/- and WT mice prior to HFD feeding, and ARC-/- mice displayed a greater increase in plasma glucose over the first 4 weeks of HFD. Plasma glucose remained elevated in ARC-/- mice after 16 weeks of HFD feeding, at which time it had returned to baseline in WT mice. Following 24 weeks of HFD, non-fasting plasma glucose in ARC-/- mice returned to baseline and was not different from WT mice. At this final time point, no differences were observed between genotypes in plasma glucose or insulin under fasted conditions or following intravenous glucose administration. However, HFD-fed ARC-/- mice exhibited significantly decreased β-cell area compared to WT mice. Thus, ARC deficiency delays, but does not prevent, metabolic adaptation to HFD feeding in mice, worsening transient HFD-induced hyperglycemia.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Templin, Andrew T.;  University of Washington - UW > Department of Medicine > Division of Metabolism, Endocrinology and Nutrition
Schmidt, Christine
Hogan, Meghan F.;  University of Washington - UW > Department of Medicine > Division of Metabolism, Endocrinology and Nutrition
Esser, Nathalie  ;  University of Washington - UW > Department of Medicine > Division of Metabolism, Endocrinology and Nutrition
Kitsis, Richard N.
Hull, Rebecca L.;  University of Washington - UW > Department of Medicine > Division of Metabolism, Endocrinology and Nutrition
Zraika, Sakeneh;  University of Washington - UW > Department of Medicine > Division of Metabolism, Endocrinology and Nutrition
Kahn, Steven E.;  University of Washington - UW > Department of Medicine > Division of Metabolism, Endocrinology and Nutrition
Language :
English
Title :
Loss of apoptosis repressor with caspase recruitment domain (ARC) worsens high fat diet-induced hyperglycemia in mice.
Publication date :
2021
Journal title :
The Journal of endocrinology
ISSN :
0022-0795
eISSN :
1479-6805
Volume :
251
Issue :
2
Pages :
125-135
Peer reviewed :
Peer reviewed
Available on ORBi :
since 19 January 2022

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