Evaluation of the Revogene GBS DS assay performance for the intrapartum screening of group B streptococcus in comparison with intrapartum culture

MEEX, Cécile; DEVEY, Anaïs; Defêche, Justine; BONTEMS, Sébastien; DESCY, Julie; HAYETTE, Marie-Pierre; SACHELI, Rosalie; MELIN, Pierrette

doi:10.1016/j.diagmicrobio.2021.115616

Article (Scientific journals)

Evaluation of the Revogene GBS DS assay performance for the intrapartum screening of group B streptococcus in comparison with intrapartum culture

MEEX, Cécile; DEVEY, Anaïs; Defêche, Justine et al.

2022 • In Diagnostic Microbiology and Infectious Disease

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/267298

DOI
10.1016/j.diagmicrobio.2021.115616

PubMed
34974352

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Keywords :

Group B streptococcus; Intrapartum screening; Revogene

Abstract :

[en] Prevention of perinatal Group B Streptococcus (GBS) transmission is crucial in our effort to prevent Earlyonset GBS disease. Here, we established the performance of the Revogene GBS DS assay for the detection of group B streptococcus on intrapartum vaginal samples in a laboratory environment using a prospective noninterventional study design. Intrapartum vaginal swabs were enriched using a selective culture method which served as study reference method. Overall, 119 patients were enrolled with an antenatal and intrapartum Group B Streptococcus colonization prevalence of 12.9% and 11.8%, respectively. Compared to intrapartum culture, the Revogene GBS DS assay had a sensitivity of 92.9% and a specificity of 99.1%, while the antenatal culture displayed a sensitivity 78.6% of and specificity of 96.2%. The Revogene GBS DS assay displayed an acceptable performance according to the European Group B Streptococcus consensus recommendations. Complementary studies in clinical practice are needed to confirm these findings.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

MEEX, Cécile ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire bactériologie

DEVEY, Anaïs ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service d'hématologie biologique et immuno-hématologie

Defêche, Justine ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Pool assistant - biologie clinique

BONTEMS, Sébastien ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire LRS

DESCY, Julie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire mycobactérie

HAYETTE, Marie-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de microbiologie clinique

SACHELI, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire CNR

MELIN, Pierrette ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de microbiologie clinique

Language :

English

Title :

Evaluation of the Revogene GBS DS assay performance for the intrapartum screening of group B streptococcus in comparison with intrapartum culture

Publication date :

2022

Journal title :

Diagnostic Microbiology and Infectious Disease

ISSN :

0732-8893

Publisher :

Elsevier, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 January 2022

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