[en] Tissue accumulation of p16INK4a-positive senescent cells is associated with age-related disorders, such as osteoarthritis (OA). These cell-cycle arrested cells affect tissue function through a specific secretory phenotype. The links between OA onset and senescence remain poorly described. Using experimental OA protocol and transgenic Cdkn2a+/luc and Cdkn2aluc/luc mice, we found that the senescence-driving p16INK4a is a marker of the disease, expressed by the synovial tissue, but is also an actor: its somatic deletion partially protects against cartilage degeneration. We test whether by becoming senescent, the mesenchymal stromal/stem cells (MSCs), found in the synovial tissue and sub-chondral bone marrow, can contribute to OA development. We established an in vitro p16INK4a-positive senescence model on human MSCs. Upon senescence induction, their intrinsic stem cell properties are altered. When co-cultured with OA chondrocytes, senescent MSC show also a seno-suppressive properties impairment favoring tissue degeneration. To evaluate in vivo the effects of p16INK4a-senescent MSC on healthy cartilage, we rely on the SAMP8 mouse model of accelerated senescence that develops spontaneous OA. MSCs isolated from these mice expressed p16INK4a. Intra-articular injection in 2-month-old C57BL/6JRj male mice of SAMP8-derived MSCs was sufficient to induce articular cartilage breakdown. Our findings reveal that senescent p16INK4a-positive MSCs contribute to joint alteration.
Tachikart, Y.; IRMB, Univ Montpellier, INSERM, Montpellier, France
Constantinides, M.; IRMB, Univ Montpellier, INSERM, Montpellier, France
Mumme, M.; IRMB, Univ Montpellier, INSERM, Montpellier, France, Clinic for Orthopedics and Traumatology, University Hospital of Basel, Basel, Switzerland
Ferreira-Lopez, R.; Hôpital Lapeyronie, Clinical immunology and Osteoarticular Diseases Therapeutic Unit, Montpellier, France
Noack, S.; Trauma Department, Hannover Medical School, Hannover, Germany
Krettek, C.; Trauma Department, Hannover Medical School, Hannover, Germany
Noël, D.; IRMB, Univ Montpellier, INSERM, Montpellier, France, Hôpital Lapeyronie, Clinical immunology and Osteoarticular Diseases Therapeutic Unit, Montpellier, France
Wang, J.; INM, Univ Montpellier, INSERM, Montpellier, France
Jorgensen, C.; IRMB, Univ Montpellier, INSERM, Montpellier, France, Hôpital Lapeyronie, Clinical immunology and Osteoarticular Diseases Therapeutic Unit, Montpellier, France
Brondello, J.-M.; IRMB, Univ Montpellier, INSERM, Montpellier, France
Language :
English
Title :
Mesenchymal stem cell senescence alleviates their intrinsic and seno-suppressive paracrine properties contributing to osteoarthritis development
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