Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms

Schellenberger, R.; Crouzet, J.; Nickzad, A.; Shu, L.-J.; Kutschera, A.; Gerster, T.; Borie, N.; Dawid, C.; Cloutier, M.; Villaume, S.; Dhondt-Cordelier, S.; Hubert, Jane; Cordelier, S.; Mazeyrat-Gourbeyre, F.; Schmid, C.; Ongena, Marc; Renault, J.-H.; Haudrechy, A.; Hofmann, T.; Baillieul, F.; Clément, C.; Zipfel, C.; Gauthier, C.; Déziel, E.; Ranf, S.; Dorey, S.

doi:10.1073/PNAS.2101366118

Article (Scientific journals)

Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms

Schellenberger, R.; Crouzet, J.; Nickzad, A. et al.

2021 • In Proceedings of the National Academy of Sciences of the United States of America, 118 (39)

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https://hdl.handle.net/2268/267263

DOI
10.1073/PNAS.2101366118

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34561304

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Keywords :

HAA; Plant immunity; Pseudomonas; Rhamnolipids

Abstract :

[en] Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from L-rhamnose and (R)-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial surface motility, biofilm development, and thus successful colonization of hosts. Here,we show that the lipidic secretome from the opportunistic pathogen Pseudomonas aeruginosa, mainly comprising RLs and HAAs, stimulates Arabidopsis immunity. We demonstrate that HAAs are sensed by the bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29), which also mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) perception, in the plant Arabidopsis thaliana. HAA sensing induces canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, although abolishing sensing by LORE, do not impair their ability to trigger plant defense. Moreover, our results show that the immune response triggered by RLs is affected by the sphingolipid composition of the plasmamembrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms. © 2021 National Academy of Sciences. All rights reserved.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Schellenberger, R.; Universite de Reims Champagne-Ardenne, Unite de Recherche Resistance Induite et Bioprotection des Plantes, Unité d'accueil 4707, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Unite sous contrat 1488, Structure Federative de Recherche Condorcet, CNRS, Federation de Recherche 3417, Reims, 51687, France

Crouzet, J.; Universite de Reims Champagne-Ardenne, Unite de Recherche Resistance Induite et Bioprotection des Plantes, Unité d'accueil 4707, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Unite sous contrat 1488, Structure Federative de Recherche Condorcet, CNRS, Federation de Recherche 3417, Reims, 51687, France

Nickzad, A.; Centre Armand-Frappier Sante Biotechnologie, Institut National de la Recherche Scientifique, Laval, QC H7V 1B7, Canada

Shu, L.-J.; Phytopathology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising-Weihenstephan, 85354, Germany

Kutschera, A.; Phytopathology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising-Weihenstephan, 85354, Germany

Gerster, T.; Phytopathology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising-Weihenstephan, 85354, Germany

Borie, N.; Universite de Reims Champagne-Ardenne, CNRS, Institut de Chimie Moleculaire, Unite Mixte de Recherche 7312, Structure Federative de Recherche Condorcet, CNRS, Federation de Recherche 3417, Reims, 51687, France

Dawid, C.; Food Chemistry and Molecular Sensory Science, School of Life Sciences Weihenstephan, Technical University of Munich, Freising-Weihenstephan, 85354, Germany

Cloutier, M.; Centre Armand-Frappier Sante Biotechnologie, Institut National de la Recherche Scientifique, Laval, QC H7V 1B7, Canada

Villaume, S.; Universite de Reims Champagne-Ardenne, Unite de Recherche Resistance Induite et Bioprotection des Plantes, Unité d'accueil 4707, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Unite sous contrat 1488, Structure Federative de Recherche Condorcet, CNRS, Federation de Recherche 3417, Reims, 51687, France

More authors (16 more)

Language :

English

Title :

Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms

Publication date :

2021

Journal title :

Proceedings of the National Academy of Sciences of the United States of America

ISSN :

0027-8424

eISSN :

1091-6490

Publisher :

National Academy of Sciences

Volume :

118

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

MESRI - Ministère de l’Enseignement supérieur, de la Recherche et de l’Innovation
NSERC - Natural Sciences and Engineering Research Council
EC - European Commission
DFG - Deutsche Forschungsgemeinschaft
FRQNT - Fonds de Recherche du Québec - Nature et Technologies
CNRS - Centre National de la Recherche Scientifique

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