Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia
Morales Yánez, Francisco Javier; Trashin, Stanislav; Sariego, Idaliaet al.
[en] Background: The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we devel‐ oped a nanobody‐based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretory‐ secretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age.
Methods: Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody‐based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre‐coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase.
Results: Of 87 samples tested, 33 (38%) scored positive for TES antigen recognition by the electrochemical magne‐ tosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19% (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil‐transmitted helminth infections.
Conclusions: Our nanobody‐based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.
Disciplines :
Biotechnology
Author, co-author :
Morales Yánez, Francisco Javier ; Vrije Universiteit Brussel - VUB > Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium
Trashin, Stanislav; Universiteit Antwerpen - UA > Department of Chemistry > AXES Research Group
Sariego, Idalia; Institute of Tropical Medicine Pedro Kouri > Department of Parasitology
Roucher, Clementine; Institute of Tropical Medicine Antwerp > Department of Biomedical Sciences > Unit of Medical Helminthology
Paredis, Linda; Institute of Tropical Medicine Antwerp > Department of Biomedical Sciences > Unit of Medical Helminthology
Chico, Martha; Fundación Ecuatoriana Para Investigación en Salud, Quito, Ecuador
De Wael, Karolien; Universiteit Antwerpen - UA > Department of Chemistry > AXES Research Group
Cooper, Philip; Universidad Internacional del Ecuador > Facultad de Ciencias Médicas, de la Salud y la Vida.
Polman, Katja; Universiteit Antwerpen - UA > Department of Biomedical Sciences > Unit of Medical Helminthology
Language :
English
Title :
Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia
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