Article (Scientific journals)
Identification of the potential active site of the septal peptidoglycan polymerase FtsW.
Li, Ying; Boes, Adrien; Cui, Yuanyuan et al.
2022In PLoS Genetics, 18 (1), p. 1009993
Peer Reviewed verified by ORBi
 

Files


Full Text
PLOSGenet.pdf
Publisher postprint (4.12 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] SEDS (Shape, Elongation, Division and Sporulation) proteins are widely conserved peptidoglycan (PG) glycosyltransferases that form complexes with class B penicillin-binding proteins (bPBPs, with transpeptidase activity) to synthesize PG during bacterial cell growth and division. Because of their crucial roles in bacterial morphogenesis, SEDS proteins are one of the most promising targets for the development of new antibiotics. However, how SEDS proteins recognize their substrate lipid II, the building block of the PG layer, and polymerize it into glycan strands is still not clear. In this study, we isolated and characterized dominant-negative alleles of FtsW, a SEDS protein critical for septal PG synthesis during bacterial cytokinesis. Interestingly, most of the dominant-negative FtsW mutations reside in extracellular loops that are highly conserved in the SEDS family. Moreover, these mutations are scattered around a central cavity in a modeled FtsW structure, which has been proposed to be the active site of SEDS proteins. Consistent with this, we found that these mutations blocked septal PG synthesis but did not affect FtsW localization to the division site, interaction with its partners nor its substrate lipid II. Taken together, these results suggest that the residues corresponding to the dominant-negative mutations likely constitute the active site of FtsW, which may aid in the design of FtsW inhibitors.
Disciplines :
Microbiology
Author, co-author :
Li, Ying 
Boes, Adrien 
Cui, Yuanyuan
Zhao, Shan
Liao, Qingzhen
Gong, Han
Breukink, Eefjan
Lutkenhaus, Joe
Terrak, Mohammed   ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Du, Shishen 
 These authors have contributed equally to this work.
Language :
English
Title :
Identification of the potential active site of the septal peptidoglycan polymerase FtsW.
Publication date :
2022
Journal title :
PLoS Genetics
ISSN :
1553-7390
eISSN :
1553-7404
Publisher :
Public Library of Science, United States - California
Volume :
18
Issue :
1
Pages :
e1009993
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 January 2022

Statistics


Number of views
83 (28 by ULiège)
Number of downloads
87 (13 by ULiège)

Scopus citations®
 
7
Scopus citations®
without self-citations
7
OpenCitations
 
5

Bibliography


Similar publications



Contact ORBi